Centro Microplate Luminometer | Microplate Reader

ULTRASENSITIVE, RELIABLE AND ACCURATE LUMINESCENCE MEASUREMENT

The Centro is a high-performance, easy-to-use and versatile microplate luminometer for both, glow and flash luminescence assays. The optimized optical system of the instrument provides lowest background and negligible crosstalk (10^-6). It can be equipped with up to 3 injectors.

The Centro XS version of the system has been built on the basis of the successful Centro, and shares with it its reliability and robustness, but has been optimized to increase its sensitivity even further: the Centro XS is the most sensitive microplate luminometer available and reliably measures less than 1.8 amol ATP per well. This makes the Centro XS the ideal partner for applications such as determination of cell viability, e.g. in tumour chemosensitivity assay, cell proliferation, antibiotic susceptibility testing or pathogen detection.

The Centro supports all basic luminescence technologies including:

• Flash-type Luminescence
• Glow-type Luminescence
• Cellular Luminescence
• Bio-Luminescence
• Chemi-Luminescence

Sensitive and reliable

Thanks to a stringent selection of photomultipliers working in photon counting mode and more than 40 years of experience building luminometers, the Centro provides highly sensitive luminescence detection: less than 5 amol ATP/well (25 fM) can be detected reliably. In the Centro XS, optics have been optimized to increase its sensitivity even further: below 1.8 amol ATP/well (9 fM).

Powerful crosstalk control – reliable results

Do you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples?  Then you´re potentially affected by a phenomenon called crosstalk, that can produce wrong results and, hence, wrong conclusions. The crosstalk reduction design of the Centro does almost eliminate this problem: with a crosstalk of 10^-6, you never have to worry about the position of your high-intensity samples in your plate.

Integrated waste pump

Thanks to its built-in waste pump, you don’t need to worry about loading microplates, waste containers or removal of injector tips for washing and filling the reagent lines; the built-in waste pump takes care of the safe removal of excess liquids.

JET injectors: highest accuracy, speed and cell-friendliness

The Centro can be equipped with up to 3 JET injectors with variable volumes in pre-measurement or measurement position*. The ultra-fast injection speed of JET injectors eliminates any delay between injection and measurement and enables measurement of fast reactions, e.g. flash luminescence.

Berthold Technologies’ JET injectors use Teflon bellows for accurate and fast injections and guarantee most efficient mixing as well as extreme longevity.

• Accurate pipetting of small volumina: JET injectors ensure the injection of large and small volumes with high accuracy and offer a precision of more than 98% over the entire volume range
• Low maintenance costs: frictionless operation for extended lifetime
• Cell-friendly performance: cell-friendly materials and negligible shear forces enable worry-free injection of cell suspensions, e.g. in Aequorin-based calcium assays

* All injectors in pre-measurement position in the Centro XS.

Convenient Software options

Our microplate readers are powered by LightCompass®, a flexible software enabling easy data acquisition, analysis and reporting using Berthold Technologies’ instruments. It has been optimized for different microplate and tube formats. LightCompass® features all the functions you can expect from an advanced scientific software, such as flexible creation of measurement protocols, curve fitting, qualitative analysis and compliance with FDA 21 CFR Part 11.

LightCompass® is available in 3 different versions: Basic, Plus and Professional. Just pick the version that better suits your research needs.

TECHNICAL DETAILS

Detectors: Low-noise photomultiplier tube in single photon counting mode
Detection technologies: Luminescence
Measurement modes: Endpoint, Kinetics, Repeated, Area scan (up to 100 x 100 points per well)
Spectral range luminescence: 340-630 nm
Injectors: Up to 3 JET injectors with frictionless operation
Injector volume: 10-100 µL in 1 µL steps
Injector precision: Better than 2% across the entire volume range
Injector accuracy: Better than 2% across the entire volume range
Microplate formats: 96- and 384-well*  *Centro  XS only
Shaking: From ambient +5° to 45° C (not available in Centro XS)
Temperature control: From ambient +5° to 45° C (not available in Centro XS)
Luminescence sensitivity: Centro: <5 amol (25 fM) ATP/well (96-well plate)/Centro XS: <1.8 amol (9 fM) ATP/well (96-well plate)
Crosstalk: 10^-6
Dynamic range: >6 orders of magnitude
Interface: Windows software, connected to computer via USB
Operating system: Windows 10
Available software packages: LightCompass®: flexible software for data acquisition, analysis and reporting.
Regulations: CE, NRTL
Power Supply: 100 – 240 VAC ±10%, 50/60 Hz
Operating Voltage: 24 VDC / 6.67 A
Temperature tolerance: Storage: 0-40° C, Operation: 15-35° C
Humidity tolerance: 10-80% non-condensing, decreasing linearly to 50 %, relative humidity up to 40 °C, decreasing linearly to 50 %, relative humidity up to 40 °C
Dimensions (W x D x H): 334 x 437 x 287 mm
Weight: 21.8 kg