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New England BioGroup has been providing professional sales representation to global brands for 30 years. If you have innovative, high-quality life science instruments and would like us to represent you, please provide your email below and we will be in contact.<\/p><\/div><\/div>

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New England BioGroup has been providing professional sales representation to global brands for 30 years. If you have innovative, high-quality life science instruments and would like us to represent you, please provide your email below and we will be in contact.

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Details No products found ]]> 3489 0 0 0 Duis aute irure dolor in velit esse cillum dolore eu fugiat nulla pariatur. occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.<\/p>","align":"left","__globals__":{"text_color":"globals\/colors?id=b8d1d84"},"_margin":{"unit":"px","top":"-10","right":"0","bottom":"0","left":"0","isLinked":false},"__dynamic__":{"editor":"[elementor-tag id=\"67e499a\" name=\"woocommerce-product-short-description-tag\" 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At New England Biogroup

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This is an example page. It's different from a blog post because it will stay in one place and will show up in your site navigation (in most themes). Most people start with an About page that introduces them to potential site visitors. It might say something like this:

Hi there! I'm a bike messenger by day, aspiring actor by night, and this is my website. I live in Los Angeles, have a great dog named Jack, and I like piña coladas. (And gettin' caught in the rain.)

...or something like this:

The XYZ Doohickey Company was founded in 1971, and has been providing quality doohickeys to the public ever since. Located in Gotham City, XYZ employs over 2,000 people and does all kinds of awesome things for the Gotham community.

As a new WordPress user, you should go to your dashboard to delete this page and create new pages for your content. Have fun!

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Who we are

Suggested text: Our website address is: https://nebg.biobm.com.

Comments

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Where your data is sent

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This is a sample page.

Overview

Our refund and returns policy lasts 30 days. If 30 days have passed since your purchase, we can’t offer you a full refund or exchange.

To be eligible for a return, your item must be unused and in the same condition that you received it. It must also be in the original packaging.

Several types of goods are exempt from being returned. Perishable goods such as food, flowers, newspapers or magazines cannot be returned. We also do not accept products that are intimate or sanitary goods, hazardous materials, or flammable liquids or gases.

Additional non-returnable items:

• Gift cards
• Downloadable software products
• Some health and personal care items

To complete your return, we require a receipt or proof of purchase.

Please do not send your purchase back to the manufacturer.

There are certain situations where only partial refunds are granted:

• Book with obvious signs of use
• CD, DVD, VHS tape, software, video game, cassette tape, or vinyl record that has been opened.
• Any item not in its original condition, is damaged or missing parts for reasons not due to our error.
• Any item that is returned more than 30 days after delivery

Refunds

Once your return is received and inspected, we will send you an email to notify you that we have received your returned item. We will also notify you of the approval or rejection of your refund.

If you are approved, then your refund will be processed, and a credit will automatically be applied to your credit card or original method of payment, within a certain amount of days.

Late or missing refunds

If you haven’t received a refund yet, first check your bank account again.

Then contact your credit card company, it may take some time before your refund is officially posted.

Next contact your bank. There is often some processing time before a refund is posted.

If you’ve done all of this and you still have not received your refund yet, please contact us at {email address}.

Sale items

Only regular priced items may be refunded. Sale items cannot be refunded.

Exchanges

We only replace items if they are defective or damaged. If you need to exchange it for the same item, send us an email at {email address} and send your item to: {physical address}.

Gifts

If the item was marked as a gift when purchased and shipped directly to you, you’ll receive a gift credit for the value of your return. Once the returned item is received, a gift certificate will be mailed to you.

If the item wasn’t marked as a gift when purchased, or the gift giver had the order shipped to themselves to give to you later, we will send a refund to the gift giver and they will find out about your return.

Shipping returns

To return your product, you should mail your product to: {physical address}.

You will be responsible for paying for your own shipping costs for returning your item. Shipping costs are non-refundable. If you receive a refund, the cost of return shipping will be deducted from your refund.

Depending on where you live, the time it may take for your exchanged product to reach you may vary.

If you are returning more expensive items, you may consider using a trackable shipping service or purchasing shipping insurance. We don’t guarantee that we will receive your returned item.

Need help?

Contact us at {email} for questions related to refunds and returns.

]]> 15 0 0 0 https://nebg.biobm.com/ Mon, 19 Feb 2024 11:30:56 +0000 https://nebg.biobm.com/?page_id=18 Cell Counters Gel Documentation Tissue Dissociators Luminometers Microplate Readers Digital Imaging Radio HPLC Detectors Microplate Washers Tissue Clearing System ELISA Automation Manufacturers We Represent

We're proud to offer a carefully curated selection of life science instruments from some of the top international manufacturers. We provide only the best instruments to labs who can't afford to compromise on the quality of their research.

Featured Products

NightOWL II LB 983 In Vivo Imaging System The NightOWL II uses an ultrasensitive back-illuminated (backlit) CCD camera with high quantum efficiency... Details NightSHADE evo In Vivo Plant Imaging System The NightSHADE evo uses an ultrasensitive back-illuminated (backlit) CCD camera with high quantum efficiency... Details QUANTOM Tx™ Microbial Cell Counter No more waiting days to count CFUs. In less than 30 seconds, the QUANTOM Tx™ scans up to 10 fields of... Details DeepLabel™ Antibody Staining Kit for Cleared Tissues DeepLabel™ Antibody Staining Kit enhances antibody penetration into large clarified tissues for vibrant... Details X-CLARITY™ Tissue Clearing Systems & Reagents The X-CLARITY™ is a collection of systems and ready-to-use reagents developed by Logos Biosystems to... Details LUNA-FX7™ Automated Cell Counter All new optics and upgraded software algorithm with high-speed and precision autofocus. Options for a... Details CELENA X|High Content Imaging System Fully automated plate and slide imaging Automated vessel handling and scanning Motorized XYZ stage, filter... Details Centro Microplate Luminometer | Microplate Reader The Centro supports all basic luminescence technologies including: Flash-type Luminescence Glow-type... Details No products found

Our Latest

Browse the newest life science instruments in our offering. New England BioGroup offers everything from digital microscopes, to cell counters, to automated ELISA systems.

Crocodile 5-in-one ELISA miniWorkstation Running your ELISA assays without automation requires an hour or more of your attention, making it difficult to plan oth... Detail Crocodile 4-in-one Assay miniWorkstation Many assays performed in microplates involve several washing, dispensing, incubation and shaking steps. Processing these... Detail Junior LB 9509 Portable Luminometer The Junior is a small portable tube luminometer which can be used for all common applications using glow type bi... Detail Lumat Luminometer for Tubes The new Lumat is a tube luminometer that uses state-of-the-art technology based on more than 40 years of experience engi... Detail NightOWL II LB 983 In Vivo Imaging System The NightOWL II uses an ultrasensitive back-illuminated (backlit) CCD camera with high quantum efficiency over... Detail NightSHADE evo In Vivo Plant Imaging System The NightSHADE evo uses an ultrasensitive back-illuminated (backlit) CCD camera with high quantum efficiency o... Detail QUANTOM Tx™ Microbial Cell Counter No more waiting days to count CFUs. In less than 30 seconds, the QUANTOM Tx™ scans up to 10 fields of view to produce... Detail DeepLabel™ Antibody Staining Kit for Cleared Tissues DeepLabel™ Antibody Staining Kit enhances antibody penetration into large clarified tissues for vibrant fluorescence 3... Detail X-CLARITY™ Tissue Clearing Systems & Reagents The X-CLARITY™ is a collection of systems and ready-to-use reagents developed by Logos Biosystems to make electrophore... Detail LUNA-FX7™ Automated Cell Counter All new optics and upgraded software algorithm with high-speed and precision autofocus. Options for a diverse range of... Detail No products found Request More Info Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed tempor diam erat, a luctus orci elementum nec. Aenean ipsum velit, placerat sed velit quis, condimentum laoreet quam. Quisque ornare in tortor nec semper. Etiam in erat sit amet erat pretium blandit sed eu purus. John DoeCEO Lorem ipsum dolor sit amet, consectetur adipiscing elit. placerat sed velit quis, condimentum laoreet quam. Quisque ornare in tortor nec semper. Etiam in erat sit amet erat pretium blandit sed eu purus. Morisa PoeCEO Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed tempor diam erat, a luctus orci elementum nec. Aenean ipsum velit, placerat sed velit quis, condimentum laoreet quam. Quisque ornare in tortor nec semper. Etiam in erat sit amet erat pretium blandit Prinas RoeaCEO

New England BioGroup

For Manufacturers

New England BioGroup has been providing professional sales representation to global brands for 30 years. If you have innovative, high-quality life science instruments and would like us to represent you, please provide your email below and we will be in contact.

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About us

our mission to position scientists to advance their research and accelerate discovery.

Our History

New England BioGroup is dedicated to supplying high quality instruments, services, software, and consumables to life science researchers in biopharmaceutical companies, universities, and other research institutions located in the New England region of the United States. It is our mission to position scientists to advance their research and accelerate discovery.

To achieve this goal, we have partnered with a small, select group of manufacturers. This enables the NE BioGroup staff to provide fast and specialized service.  We take pride in our ability to match customers with the right products to meet their research needs while helping labs make the most out of their budgets.

Empowering innovation, shaping futures.

To achieve this goal, we have partnered with a small, select group of manufacturers. This enables the NE BioGroup staff to provide fast and specialized service.  We take pride in our ability to match customers with the right products to meet their research needs while helping labs make the most out of their budgets.

 

We’re also a small, family-owned business that takes pride in its values.  When you work with us, you can be sure that you’ll mean more to us than just a sale.

Contact Us

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed tempor diam erat, a luctus orci elementum nec. Aenean ipsum velit, placerat sed velit quis, condimentum laoreet quam. Quisque ornare in tortor nec semper. Etiam in erat sit amet erat pretium blandit sed eu purus. John DoeCEO Lorem ipsum dolor sit amet, consectetur adipiscing elit. placerat sed velit quis, condimentum laoreet quam. Quisque ornare in tortor nec semper. Etiam in erat sit amet erat pretium blandit sed eu purus. Morisa PoeCEO Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed tempor diam erat, a luctus orci elementum nec. Aenean ipsum velit, placerat sed velit quis, condimentum laoreet quam. Quisque ornare in tortor nec semper. Etiam in erat sit amet erat pretium blandit Prinas RoeaCEO

Brands We Love Most

At New England Biogroup

For Manufacturers

New England BioGroup has been providing professional sales representation to global brands for 30 years. If you have innovative, high-quality life science instruments and would like us to represent you, please provide your email below and we will be in contact.

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For Manufacturers

New England BioGroup has been providing professional sales representation to global brands for 30 years. If you have innovative, high-quality life science instruments and would like us to represent you, please provide your email below and we will be in contact.

]]> 3084 0 0 0 New England BioGroup has been providing professional sales representation to global brands for 30 years. If you have innovative, high-quality life science instruments and would like us to represent you, please provide your email below and we will be in contact.<\/p>","align_mobile":"center","__globals__":{"text_color":"globals\/colors?id=b8d1d84","typography_typography":""},"align":"center","_margin":{"unit":"px","top":"10","right":"0","bottom":"40","left":"0","isLinked":false},"text_color":"#232323","typography_typography":"custom","typography_font_family":"Poppins","typography_font_size":{"unit":"px","size":16,"sizes":[]},"typography_font_size_tablet":{"unit":"px","size":16,"sizes":[]},"typography_font_size_mobile":{"unit":"px","size":16,"sizes":[]},"typography_font_weight":"300","typography_text_transform":"none","typography_font_style":"normal","typography_text_decoration":"none","typography_line_height":{"unit":"px","size":22,"sizes":[]},"typography_line_height_tablet":{"unit":"px","size":"","sizes":[]},"typography_line_height_mobile":{"unit":"px","size":"","sizes":[]},"_margin_mobile":{"unit":"px","top":"0","right":"0","bottom":"30","left":"0","isLinked":false},"_element_width":"initial","_element_custom_width":{"unit":"%","size":90,"sizes":[]},"_element_custom_width_mobile":{"unit":"%","size":100,"sizes":[]}},"elements":[],"widgetType":"text-editor"},{"id":"3bd6ca2","elType":"widget","settings":{"form_name":"New Form","form_fields":[{"custom_id":"field_bcacc37","field_type":"email","required":"true","field_label":"Email","placeholder":"Your Email Address","width":"80","width_mobile":"80","_id":"bcacc37","file_sizes":"","file_types":"","allow_multiple_upload":"","max_files":"","acceptance_text":"","checked_by_default":"","field_min":"","field_max":"","min_date":"","max_date":"","use_native_date":"","use_native_time":"","previous_button":"","next_button":""}],"show_labels":"","button_text":"","button_width":"20","selected_button_icon":{"value":"fas fa-long-arrow-alt-right","library":"fa-solid"},"button_icon_align":"right","email_content":"[all-fields]","email_content_2":"[all-fields]","success_message":"The form was sent successfully.","error_message":"An error occured.","required_field_message":"This field is required.","invalid_message":"There's something wrong. 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Consideration #1: Your Lab's Needs

The first question to ask is "what does my lab need" - what are the most basic requirements that we have for a gel documentation system?  Are you just going to be running protein gels with ethidium bromide?  Will you be imaging gels using fluorescence, or chemiluminescence?  Will you require multi-channel imaging capabilities?  How large of gels will you need to be able to document?  Get the basic requirements set in stone before you think about making a purchase, and consider things that you may realistically need to do in the future.  

Consideration #2: Lighting

Lighting usually gets addressed when labs are considering their basic requirements, as there's generally no way around it.  If you're doing chemiluminescence westerns, you need a gel doc with chemiluminescence.  If you'll be staining protein gels with Coomassie blue, you'll need light transillumination.  You get the idea.  

Consideration #3: Price

As most labs have a budget to conform to, one of the first things that usually gets discussed is price.  A basic UV-vis gel documentation system with a good feature set can generally be purchased for under $10,000.  Add features like high-resolution cameras, top-end analysis software, multi-channel imaging, chemiluminescence or fluorescence, and you may need to budget more than that (depending on the particular feature set you want, of course).  

Consideration #4: Camera & Resolution

Most  gel doc systems have cameras with resolutions between 0.5 and 8 megapixels, with anything under 2MP generally considered "entry level".  As higher resolution and deeply cooled (cooled CCD cameras) systems can significantly increase the cost, it's probably best to discuss your needs and applications with your sales rep so you can determine a good resolution than assume that you need a high-resolution model.  Likewise, assuming that an entry level camera will be "good enough" can be disastrous, so unless you're certain of your needs, discuss your applications with your sales specialist.  

Consideration #5: Software

Do you want your gel documentation system to simply acquire images, or do you want analysis?  If you want analysis, how much analysis do you want your gel doc system to be able to perform?  Are you going to want the software to run on the device itself or on a computer?  Will each lab member need their own copy of the analysis software?  Important questions to ask include how many software licenses come with the gel-doc (and / or how expensive additional licenses are), how often the software is updated, and how long you get free updates for.  

Consideration #6: Automation

Some gel docs are very easy to use, others not so much.  The difference most often comes down to automation.  How many settings need to be adjusted each time an image is taken?  Fully automated systems will be more expensive, of course, but they'll increase your efficiency and throughput and also require less training.   Of course there are other considerations when purchasing a gel doc as well, such as access to the data, quality and format of the output, physical size, warranty, and a system's ability to be upgraded.  Hopefully with this in mind, you'll be able to find a gel documentation system that fits both your current and future needs as well as your budget. And if you ever need some help, just ask us!]]> 218 0 0 0 https://nebg.biobm.com/2012/03/07/syngenes-innovative-ultrabright-led-blue-light-transilluminator/ Wed, 07 Mar 2012 00:57:46 +0000 http://www.nebiogroup.com/?p=570 Offers Exceptional Imaging of Fluorescent DNA Gels without Compromising Safety Syngene, a world-leading manufacturer of image analysis solutions, today introduced the UltraBright-LED transilluminator, a new generation of blue light transilluminator designed for safely imaging fluorescently labeled gels (up to 20cm x 16cm) on the bench or inside Syngene G:BOX imaging systems. The UltraBright-LED uses two high intensity LED arrays which produce uniform, bright excitation at 470nm, and can illuminate dyes that excite at 420 to 480nm. Therefore, the system is suitable for visualizing small amounts of Ethidium Bromide and non-toxic DNA stains including Syngene’s new UltraSafe Blue™ dye, SYBR Safe and GelGreen, as well as protein stains such as SYPRO Ruby and Pro-Q Diamond. Since the UltraBright-LED uses blue light, researchers imaging non-toxic stains can use the system directly on the bench and as it is compact and light weight it takes up very little space and is easy to move. The UltraBright-LED features a special filter in its lid which enhances band contrast and provides optimum viewing conditions. The transilluminator has been designed to easily slide in and out of any Syngene G:BOX imaging system, making the UltraBright-LED ideal for visualizing faint bands, allowing precise band cutting and production of high quality images for analysis and publication. Laura Sullivan, Syngene’s Divisional Manager stated: “Blue light transilluminators are an alternative to UV when scientists work with non-toxic fluorescent dyes. The advantage of using blue light is that it is a “safe” light source which can be used on the bench, as it is not as harmful as UV and does not photo-nick DNA samples.” Laura continued, “We are delighted to introduce the UltraBright-LED blue light transilluminator as it complements our UltraSlim-LED transilluminator for use in Syngene’s In:Genius3 and U:Genius3 gel doc systems. The UltraBright-LED transilluminator when used in combination with a G:BOX system, is an excellent tool for researchers looking to generate outstanding gel images without compromising their DNA quality or their safety.” The innovative Syngene blue light transilluminators are sold exclusively in New England by New England BioGroup, LLC.]]> 570 0 0 0 https://nebg.biobm.com/2012/03/08/learn-more-about-nexcelom-bioscience-manufacturer-of-cellometer-automated-cell-counters/ Thu, 08 Mar 2012 02:27:25 +0000 http://www.nebiogroup.com/?p=576 In 2003, the founders of Nexcelom introduced the CP2, a convenient tool to assist with manual cell counting. According to Dr. Jean Qiu, founder and CTO at Nexcelom.   One day a customer at the NIH asked us to 'make counting under the microscope go away.' We invented Cellometer Auto T4 to measure cell concentration and viability. Since then, Nexcelom has expanded the product range to accommodate researchers analyzing small cells, primary cells, and researchers looking to perform cell-based assays. With multiple patents pending, Nexcelom is a clear leader and innovator in the field of image-based cytometry for cell analysis.

Proven Performance

Thousands of customers, including the top ten pharmaceutical companies, have come to rely on Cellometer instruments for automated cell concentration & viability analysis. The Cellometer has provided a much faster and more accurate method of counting the 100s of samples we need to set up for flow cytometry analysis." - Dr. Janice Pluth, Lawrence Berkeley Laboratory From the large breweries performing yeast fermentation monitoring to specialized pharmaceutical labs analyzing fragile hepatocytes for toxicology studies, researchers in a wide range of industries have integrated Cellometer instruments into their laboratory testing.

Dedicated Applications Support

Nexcelom is focused on providing novel tools to meet the specific needs of today's researchers. Our applications specialists are in the field with customers every day, analyzing new cell types, validating new cell-based assays, and exploring new therapeutic areas. Through joint application development, we learn from our customers, the experts in their fields, and assist with integration into their current work flows."- Dr. Bo Lin, Director of Applications Development Our experienced technical support and applications specialists are committed to ensuring products work successfully in the customer's hands and are always eager to provide support via the phone, internet, and on-site visits.

Continued Growth and Innovation

The new Vision CBA Analysis System fulfills a growing customer need for a simpler, more user-friendly method for analysis of apoptosis, cell cycle, and other cell-based assays, as an alternative to flow cytometry. This new system exemplifies how we extend our Cellometer technology platform to meet the evolving customer needs. In this case, we enable cell based assays with a very limited amount of cells, as we continue to make an impact with cytometry on the biology lab bench." - Dr. Peter Li, President and CEO Nexcelom will continue to develop novel products, including enhanced instrumentation and specialized reagents, to meet emerging customer needs.  New England BioGroup, LLC has been proudly selling the Cellometer automated cell counters since their launch in 2005.    If you would like to discuss which Cellometer best fits your needs, or if you would like to arrange a demonstration in the lab, contact us.

]]> 576 0 0 0 https://nebg.biobm.com/2012/03/14/gel-documentation-101-agarose-gel-electrophoresis/ Wed, 14 Mar 2012 01:07:37 +0000 http://www.nebiogroup.com/?p=584 Applications

• Estimation of the size of DNA molecules following restriction enzyme digestion, e.g. in restriction mapping of cloned DNA.
• Analysis of PCR products, e.g. in molecular genetic diagnosis or genetic fingerprinting
• Separation of restricted genomic DNA prior to Southern transfer, or of RNA prior to Northern transfer.

Agarose gels are easily cast and handled compared to other matrices and nucleic acids are not chemically altered during electrophoresis. Samples are also easily recovered. After the experiment is finished, the resulting gel can be stored in a plastic bag in a refrigerator. [caption id="attachment_593" align="alignright" width="300" caption="A Galileo Bioscience or Owl Scientific mini gel electrophoresis tank can be used to run DNA gels"][/caption] How to make 1% agarose gel: In a small beaker, mix 32mL of purified H2O, 4mL of 10x TBE buffer or 10x whichever buffer going to be used in electrophoresis, and 0.4g of agarose powder. Heat on hot plate, or microwave, until solution is almost boiling (about 98 degrees Celsius). Immediately pour solution into electrophoresis mold or Galileo Bioscience gel casting tray with notch (well) comb intact, let settle for approximately 30 minutes, or until it appears firm. Be careful, a gel which is perfectly molded is still extremely fragile. Remove notch (well) comb carefully, but quickly. Wrap in plastic and place in refrigerator if gel is not for immediate use. There are limits to electrophoretic techniques. Since passing current through a gel causes heating, gels may melt during electrophoresis. Electrophoresis is performed in buffer solutions to reduce pH changes due to the electric field, which is important because the charge of DNA and RNA depends on pH, but running for too long can exhaust the buffering capacity of the solution. Further, different preparations of genetic material may not migrate consistently with each other, for morphological or other reasons.  Gel electrophoresis can be performed using a Galileo Bioscience gel electrophoresis system, or similar horizontal gel electrophoresis systems.

Factors affecting migration of nucleic acids

The most important factor is the length of the DNA molecule, smaller molecules travel faster. But conformation of the DNA molecule is also a factor. To avoid this problem linear molecules are usually separated, usually DNA fragments from a restriction digest, linear DNA PCR products, or RNAs. Increasing the agarose concentration of a gel reduces the migration speed and enables separation of smaller DNA molecules. The higher the voltage, the faster the DNA moves. But voltage is limited by the fact that it heats the gel and ultimately causes it to melt. High voltages also decrease the resolution (above about 5 to 8 V/cm). Conformations of a DNA plasmid that has not been cut with a restriction enzyme will move with different speeds (slowest to fastest: nicked or open circular, linearised, or supercoiled plasmid).

Visualization: ethidium bromide (EtBr) and dyes

The most common dye used to make DNA or RNA bands visible for agarose gel electrophoresis is ethidium bromide, usually abbreviated as EtBr. It fluoresces under UV light when intercalated into DNA (or RNA). By running DNA through an EtBr-treated gel and visualizing it with UV light, any band containing more than ~20 ng DNA becomes distinctly visible. EtBr is a known mutagen, however, so safer alternatives are available. Even short exposure of nucleic acids to UV light causes significant damage to the sample. UV damage to the sample will reduce the efficiency of subsequent manipulation of the sample, such as ligation and cloning. If the DNA is to be used after separation on the agarose gel, it is best to avoid exposure to UV light by using a blue light excitation source such as the Ultraslim Blue Light Transilluminator or Ultra Bright Blue Light Transilluminator from Syngene and available in New England through New England BioGroup, LLC  . A blue excitable stain is required, such as one of the SYBR Green or GelGreen stains.  Gels with this stain can be visualized using a Syngene gel documentation system like the U:Genius, InGenius, GENi, and [caption id="attachment_594" align="alignleft" width="300" caption="A Syngene, BioRad, UVP, Carestream Healthcare or other gel doc system can be used for gel documentation and, if offered, image analysis."][/caption] G:BOX. Blue light is also better for visualization since it is safer than UV (eye-protection is not such a critical requirement) and passes through transparent plastic and glass. This means that the staining will be brighter even if the excitation light goes through glass or plastic gel platforms. SYBR Green I is another dsDNA stain, produced by Invitrogen. It is more expensive, but 25 times more sensitive, and possibly safer than EtBr, though there is no data addressing its mutagenicity or toxicity in humans.[3]  Gels with this stain can be visualized using a Syngene gel documentation system like the U:Genius, InGenius, GENi, and G:BOX. SYBR Safe is a variant of SYBR Green that has been shown to have low enough levels of mutagenicity and toxicity to be deemed nonhazardous waste under U.S. Federal regulations.[4] It has similar sensitivity levels to EtBr,[4] but, like SYBR Green, is significantly more expensive. In countries where safe disposal of hazardous waste is mandatory, the costs of EtBr disposal can easy outstrip the initial price difference, however. Since EtBr stained DNA is not visible in natural light, scientists mix DNA with negatively charged loading buffers before adding the mixture to the gel. Loading buffers are useful because they are visible in natural light (as opposed to UV light for EtBr stained DNA), and they co-sediment with DNA (meaning they move at the same speed as DNA of a certain length). Xylene cyanol and Bromophenol blue are common loading buffers; they run about the same speed as DNA fragments that are 5000 bp and 300 bp in length respectively, but the precise position varies with percentage of the gel. Other less frequently used progress markers are Cresol Red and Orange G which run at about 125 bp and 50 bp. Visualization can also be achieved by transferring DNA to a nitrocellulose membrane followed by exposure to a hybridization probe. This process is termed southern blotting.

Percent agarose and resolution limits

Agarose gel electrophoresis on a system like the Galileo Bioscience or Thermo Owl Scientific gel electrophoresis gel running system can be used for the separation of DNA fragments ranging from 50 base pair to several megabases (millions of bases) using specialized apparatus. The distance between DNA bands of a given length is determined by the percent agarose in the gel. The disadvantage of higher concentrations is the long run times (sometimes days). Instead high percentage agarose gels should be run with a pulsed field electrophoresis (PFE), or field inversion electrophoresis. Most agarose gels are made with between 0.7% (good separation or resolution of large 5–10kb DNA fragments) and 2% (good resolution for small 0.2–1kb fragments) agarose dissolved in electrophoresis buffer. Up to 3% can be used for separating very tiny fragments but a vertical polyacrylamide gel is more appropriate in this case. Low percentage gels are very weak and may break when you try to lift them. High percentage gels are often brittle and do not set evenly. 1% gels are common for many applications.

 Buffers

There are a number of buffers used for agarose electrophoresis. The most common being:Tris/Acetate/EDTA (TAE), Tris/Borate/EDTA (TBE) and lithium borate (LB). TAE has the lowest buffering capacity but provides the best resolution for larger DNA. This means a lower voltage and more time, but a better product. LB is relatively new and is ineffective in resolving fragments larger than 5 kbp; However, with its low conductivity, a much higher voltage could be used (up to 35 V/cm), which means a shorter analysis time for routine electrophoresis. As low as one base pair size difference could be resolved in 3 % agarose gel with an extremely low conductivity medium (1 mM Lithium borate).[5]

 Analysis

After electrophoresis the gel is illuminated with an ultraviolet lamp (usually by placing it on a Syngene UV transilluminator light box, while using protective gear to limit exposure to ultraviolet radiation) to view the DNA bands. The ethidium bromide fluoresces reddish-orange in the presence of DNA. The DNA band can also be cut out of the gel, and can then be dissolved to retrieve the purified DNA. The gel can then be photographed usually with a digital or polaroid camera. Although the stained nucleic acid fluoresces reddish-orange, images are usually shown in black and white (see figures). Gel electrophoresis research often takes advantage of software-based image analysis tools, such as Syngene GeneTools image analysis software, Nonlinear Dynamincs TotalLab or Phoretix software, NIH ImageJ, or Bio-Rad Image Lab or Quantity One image analysis software.

Typical method

Materials Typically 10-30 μl/sample of the DNA fragments to separate are obtained, as well as a mixture of DNA fragments (usually 10-20) of known size (after processing with DNA size markers either from a commercial source or prepared manually).

• Buffer solution, usually TBE buffer or TAE 1.0x, pH 8.0
• Agarose
• An ultraviolet-fluorescent dye, ethidium bromide, (5.25 mg/ml in H2O). The stock solution be careful handling this. Alternative dyes may be used, such as SYBR Green.
• Nitrile rubber gloves. Latex gloves do not protect well from ethidium bromide
• A color marker dye containing a low molecular weight dye such as "bromophenol blue" (to enable tracking the progress of the electrophoresis) and glycerol (to make the DNA solution denser so it will sink into the wells of the gel).
• A gel rack such as a Bio-Rad, Owl Scientific or Galileo Bioscience horizontal gel electrophoresis running system.
• A "comb"
• Power Supply
• UV lamp or UV lightbox or a gel doc system, such as a Syngene, Bio-Rad, UVP, Carestream Healthcare or other gel documentation system to visualize DNA in the gel.

Preparation

There are several methods for preparing gels. A common example is shown here. Other methods might differ in the buffering system used, the sample size to be loaded, the total volume of the gel (typically thickness is kept to a constant amount while length and breadth are varied as needed). Most agarose gels used in modern biochemistry and molecular biology are prepared and run horizontally.

1. Make a 1% agarose solution in 100ml TAE, for typical DNA fragments (see figures). A solution of up to 2-4% can be used if you analyze small DNA molecules, and for large molecules, a solution as low as 0.7% can be used.
2. Carefully bring the solution just to the boil to dissolve the agarose, preferably in amicrowave oven.
3. Let the solution cool down to about 60 °C at room temperature, or water bath. Stir or swirl the solution while cooling.

Wear gloves from here on, ethidium bromide is a mutagen!

1. Add 5 µl ethidium bromide (from a stock solution of 10 mg/ml) per 100 ml gel solution for a final concentration of 0.5 µg/ml. Be very careful when handling the concentrated stock. Some researchers prefer not to add ethidium bromide to the gel itself, instead soaking the gel in an ethidium bromide solution after running, in this case the ethidium bromide solution has final concentration of 0.5 µg/ml.
2. Stir the solution to disperse the ethidium bromide, then pour it into the gel rack.
3. Insert the comb at one side of the gel, about 5–10 mm from the end of the gel.
4. When the gel has cooled down and become solid, carefully remove the comb. The holes that remain in the gel are the wells or slots.
5. Put the gel, together with the rack, into a tank with TAE. Ethidium bromide at the same concentration can be added to the buffer. The gel must be completely covered with TAE, with the slots at the end electrode that will have the negative current.

Procedure

After the gel has been prepared, use a micropipette to inject about 2.5 µl of stained DNA (a DNA ladder is also highly recommended). Close the lid of the electrophoresis chamber and apply current (typically 100 V for 30 minutes with 15 ml of gel). The colored dye in the DNA ladder and DNA samples acts as a "front wave" that runs faster than the DNA itself. When the "front wave" approaches the end of the gel, the current is stopped. The DNA is stained with ethidium bromide, and is then visible under ultraviolet light.

1. The agarose gel with three slots/wells (S).
2. Injection of DNA ladder (molecular weight markers) into the first slot.
3. DNA ladder injected. Injection of samples into the second and third slot.
4. A current is applied. The DNA moves toward the positive anode due to the negative charges on its phosphate backbone.
5. Small DNA strands move fast, large DNA strands move slowly through the gel. The DNA is not normally visible during this process, so the marker dye is added to the DNA to avoid the DNA being run entirely off the gel. The marker dye has a low molecular weight, and migrates faster than the DNA, so as long as the marker has not run past the end of the gel, the DNA will still be in the gel.
6. Add the color marker dye to the DNA ladder.

References

Kryndushkin DS, Alexandrov IM, Ter-Avanesyan MD, Kushnirov VV (2003). "Yeast [PSI+] prion aggregates are formed by small Sup35 polymers fragmented by Hsp104". Journal of Biological Chemistry 278: 49636. doi:10.1074/jbc.M307996200.

Sambrook J, Russel DW (2001). Molecular Cloning: A Laboratory Manual 3rd Ed. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, NY.

SYBR Green I Nucleic Acid Gel Stain

4.0 4.1 SYBR Safe DNA Gel Stain

↑ Brody, J.R., Kern, S.E. (2004): History and principles of conductive media for standard DNA electrophoresis. Anal Biochem. 333(1):1-13. PMID 15351274 PDF

 

Copyright Information

This article is distributed under the Creative Commons Attribution/Share-Alike License. For information on the contributors, please see the original Wikipedia article.  It has been adapted and modified for commercial use by New England BioGroup, LLC under the Creative Commons Legal Code (Full License).

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]]> 3146 0 0 0 https://nebg.biobm.com/2012/02/29/which-cellometer-automated-cell-counter-is-right-for-me/ Wed, 29 Feb 2012 20:22:08 +0000 http://www.nebiogroup.com/?p=340 Cellometer Auto T4 The Cellometer AutoT4 is the original brightfield imaging based automated cell counter. Some of our customers describe the AutoT4 as a digital hemacytometer. The system uses brightfield excitation and is ideal for cancer cell lines. Over three hundred cell lines have been optimized in the Cellometer Auto T4 software. Cell types such as PBMCs, lymphocyes, and spleenocytes can be counted in the Auto T4. In fact, we are often told by customers who have evaluated these cell types in other imaging based automated cell counters that the Auto T4 delivers the best results. That said, we would encourage customers to look more closely at our Cellometer Auto 2000, which is optimized for those cell types and more. Total cell counts and viability counts using Trypan blue stain can be performed with the Cellometer Auto T4. The AutoT4 also offers single button cell size analysis and cell size histogram generation, morphology observation, data archiving, and automatic data export into Microsoft Excel.

Cellometer Auto 2000

The Cellometer Auto 2000 is an "all in one" automated cell counter.  The Auto 2000 contains brightfield and dual channel fluorescent imaging.  The Auto 2000 can perform every application need addressed on the Cellometer Auto T4 and, using fluorescence, the system can is optimized to also work with primary cell lines, cell lines that contain debris, blood cells such as PBMCs.  The system can also be used to perform a total white blood cell count (WBC count) using whole blood!  Users can now work with acridine orange (AO), a membrane permeable nucleic acid stain useful for fluorescent total cell counts and/or propidum iodide (PI), a membrane permeable nucleic acid binding strain useful for fluorescent viability counts.   Users can also apply a mixture of both stains (AO/PI) to perform a fluorescent live/dead viability count in the same sample.  Another unique feature is the system works with a larger imaging area, making it ideal for labs that routinely count lower concentrations of cells.  The built-in PC is very fast and cell counts can typically be performed in <30 seconds once image acquisition is complete.

Cellometer Vision CBA

The Cellometer Vision CBA offers the end user the highest level of sensitivity and flexibility in an imaging based automated cell counter.  The Vision offers brightfield and dual channel fluorescent excitation.  In addition, the system allows for the fluorescent optic modules to be interchangeable, opening up a stunning array of cell based assay capabilities to researcher.  The Vision can perform cell cycle analysis (using only 20 ul of sample!!!), cell proliferation analysis, and help determine transfection rates in GFP, RFP and YFP.   It can also be used for apoptosis analysis (using Anexin V) as well as counting primary hepatocytes and adipocytes.  The Cellometer Vision CBA software also allows the researcher to offers advanced data reporting and generates the same sophisticated data output as most flow-based instruments, with the added advantage of cell images. Automated data output includes fluorescent images, dot plots, and histograms. Users have the option to modify the automatic gating with instant visual display and data update. Researchers can characterize cell populations with 1-step data analysis and reporting.

Cellometer Auto M10 and Auto X4

The Cellometer Auto M10 is a brightfield only yeast and platelet counter.  The instrument contains a camera with 10X magnification.  The Cellometer Auto X4 is a brightfield and single channel fluorescent automated cell counter.

So which is right for me?

Good question!  Hopefully, this information will "point you in the right direction".  Ideally, it is best to discuss this with a sales specialist at New England BioGroup.  We're here for you! Give us a call or drop us a line to chat or arrange a product demonstration in your lab.]]> 3180 0 0 0 https://nebg.biobm.com/2012/02/28/new-gbox-f3-automated-gel-imaging-system/ Tue, 28 Feb 2012 11:47:06 +0000 http://www.nebiogroup.com/?p=373 Rapid, Accurate Analysis of DNA and Protein Gels [caption id="attachment_377" align="alignright" width="180" caption="G:BOX F3 High Resolution Gel Documentation System"][/caption] Syngene, a world-leading manufacturer of image analysis solutions is delighted to introduce the G:BOX F3, a new gel imaging system, designed for scientists that want a rapid, versatile method of automatically imaging and analyzing DNA, RNA and proteins in gels. The G:BOX F3 features a high-resolution 3.8 million pixel CCD camera with a motor driven lens and built in filter wheel contained in a compact darkroom. This automated system is controlled by Syngene’s intuitive GeneSys imaging software. The software saves time and effort by automatically setting up the optimum lighting and filters from its unique database of imaging conditions for every commercially available DNA, RNA and protein dye. Users can in one screen touch, choose their preferred imaging conditions and the G:BOX F3 will aut0matically capture a high-quality gel image. Inside the G:BOX F3 system’s safe, light tight cabinet there is overhead Epi white light. Epi UV light, a UV transilluminator and visible light converter options can also be fitted. For laboratories where safety and UV sample damage are an issue, Syngene offers an UltraBright LED blue light transilluminator, which slides out of the darkroom to aid viewing and band cutting. Additionally, thistransilluminator can be fitted with an optional visible light converter screen for viewing protein gels. The G:BOX F3 system also includes unlimited copies of GeneTools image analysis software to enable each researcher in the laboratory to use their own computer to rapidly generate quantitative analysis of their protein and DNA gels in Laura Sullivan, Syngene’s Divisional Manager stated: “The G:BOX F3 is a well priced system that is suited to busy labs where researchers are working with a range of different gels. It is ideal for experienced users and novices alike as they only have to know what type of gel they have run and what it is stained with, and the system will select and set up the right imaging conditions for them. Since the technology is so versatile, this makes the G:BOX F3 system the best, budget, automated gel imaging technology currently on the market.”    ]]> 3181 0 0 0 https://nebg.biobm.com/2012/06/09/cell-counting-101-the-hemacytometer/ Sat, 09 Jun 2012 09:44:18 +0000 http://www.nebiogroup.com/?p=634 hemocytometer is a device originally designed for the counting of blood cells and is now also used to count other types as well as other microscopic particles. The hemocytometer was invented by Louis-Charles Malassez and consists of a thick glass microscope slide with a rectangular indentation that creates a chamber. This chamber is engraved with a laser-etched grid of perpendicular lines. The device is carefully crafted so that the area bounded by the lines is known, and the depth of the chamber is also known. It is therefore possible to count the number of cells or particles in a specific volume of fluid, and thereby calculate the concentration of cells in the fluid overall.

Principles

The ruled area of the hemocytometer consists of several, large, 1 x 1 mm (1 mm²) squares. These are subdivided in 3 ways; 0.25 x 0.25 mm (0.0625 mm²), 0.25 x 0.20 mm (0.05 mm²) and 0.20 x 0.20 mm (0.04 mm²). The central, 0.20 x 0.20 mm marked, 1 x 1 mm square is further subdivided into 0.05 x 0.05 mm (0.0025 mm²) squares. The raised edges of the hemocytometer hold the coverslip 0.1 mm off the marked grid. This gives each square a defined volume.

Dimensions	Area	Volume at 0.1mm depth
1 x 1 mm	1 mm2	100 nl
0.25 mm x 0.25 mm	0.0625 mm2	6.25 nl
0.25 mm x 0.20 mm	0.05 mm2	5 nl
0.20 mm x 0.20 mm	0.04 mm2	4 nl
0.05 mm x 0.05 mm	0.0025 mm2	0.25 nl

  The cell-sized structures counted lie between the middle of the three lines on the top and right of the square and the inner of the three lines on the bottom and left of the square.  In an improved Neubauer hemocytometer (common medium), the total number of cells per ml can be discovered by simply multiplying the total number of cells found in the hemocytometer grid (area equal to the redsquare in picture on right) by 10⁴ (10000).

Usage

Ensure that the special coverslip provided with the counting chamber (thicker than standard coverslips and with a certified flattness) is properly positioned on the surface of the counting chamber. When the two glass surfaces are in proper contact Newton's rings can be observed. If so, the cell suspension is applied to the edge of the coverslip to be sucked into the void by capillary action which completely fills the chamber with the sample. Looking at the chamber through a microscope, the number of cells in the chamber can be determined by counting. Different kinds of cells can be counted separately as long as they are visually distinguishable. The number of cells in the chamber is used to calculate the concentration or density of the cells in the mixture the sample comes from. It is the number of cells in the chamber divided by the chamber's volume (the chamber's volume is known from the start), taking account of any dilutions and counting shortcuts:

      In the most common design, the volume of each large square (made up from 4x4 green squares in the picture) is 0.1 mm³. The cells in four large (red in figure above) squares are counted and cells over or touching the lines on top and on the left are counted, but cells over or touching the right or bottom lines are ignored. The concentration in cells per ml = cells in four red, large squares/4 × 10,000.^[1] Hemocytometers are often used to count blood corpuscles, organelles within cells, blood cells in cerebrospinal fluid after performing a lumbar puncture, or other cell types in suspension. Anchorage-dependent cells can also be counted if subjected to trypsinization prior to counting. Using a special hemocytometer with a depth of 0.02mm smaller particles such as sperm, yeast or bacteria can be counted. Using the ruling described above the volumes are only 1/5 compared to the standard 0.1mm deep chamber. As it is difficult to distinguish between living and dead organisms unless particular stains are used to distinguish viable from non-viable cells this results in a 'total count' of the bacteria. In case of bigger cells the number of dead (permeabilised) cells in the sample can be obtained by adding Trypan blue. Fluorescent dyes give better discrimination particularly when looking at bacteria. A common mistake is to add the sample to the counting chamber before adding the cover glass. This risks that the cells could sediment / stick to the glass or some volume to evaporate before the coverslip is placed on top resulting in an overestimation of the cell concentration. Sedimentation is less of a problem with bacteria but evaporation has to be kept to a minimum

Requirements

• The original suspension must be mixed thoroughly before taking a sample. This ensures the sample is representative, and not just an artifact of the particular region of the original mixture it was drawn from.
• An appropriate dilution of the mixture with regard to the number of cells to be counted should be used. If the sample is not diluted enough, the cells will be too crowded and difficult to count. If it is too dilute, the sample size will not be enough to make strong inferences about the concentration in the original mixture.
• By performing a redundant test on a second chamber, the results can be compared. If they differ greatly, the method of taking the sample may be unreliable (e.g., the original mixture is not mixed thoroughly).

References: ^ Strober W (2001). "Monitoring cell growth". In Coligan JE, Bierer BE, Margulies DH, Sherach EM, Strober W. Current Protocols in Immunology. 5. USA: John Wiley & Sons. p. A.2A.1.DOI:10.1002/0471142735.ima03as21.

Copyright Information

This article is distributed under the Creative Commons Attribution/Share-Alike License. For information on the contributors, please see the original Wikipedia article.  It has been adapted and modified for commercial use by New England BioGroup, LLC under the Creative Commons Legal Code (Full License).

 ]]> 3182 0 0 0 https://nebg.biobm.com/2012/06/09/counting-primary-hepatocytes-using-the-cellometer-vision/ Sat, 09 Jun 2012 10:33:38 +0000 http://www.nebiogroup.com/?p=712  are the [caption id="attachment_714" align="alignright" width="288" caption="The Cellometer Vision (or Triangle Research Labs Gigacyte Hepatometer) can be used to successfully count primary hepatocytes. After successful primary hepatocyte cell isolation, 20 ul of sample, mixed with AO/PI fluorescent stains, can be placed into the Cellometer cell counting chamber for accurate and reproducible counts."][/caption] predominant cell in the liver, comprising about 60% of liver cells and 80% of liver mass. One of the main hepatocyte functions is the production of bile. Hepatocytes are also responsible for the regulation of metabolism, detoxification, and the manufacture of important circulating proteins, making them a critical component of studies involving liver disease, drug metabolism, and toxicology.  Primary hepatocyte cell isolation remains a challenge for researchers. Primary hepatocytes are very difficult to maintain in culture, displaying a rapid decline in viability and liver-specific functions. Optimization of primary hepatocyte culture conditions, including extracellular matrices (ECM), culture media / supplements, and co-culture with other cell types required for the normal cell-cell interactions displayed in vivo (micro-patterned co-cultures, MPCC) have enabled researchers to maintain hepatocyte viability and function for several weeks in culture. Recent studies have also proven cryopreservation (for up to 4 years) as a viable method of maintaining access to viable, functional primary hepatocytes. Some laboratories produce their own primary hepatocytes.  Primary hepatocyte cell lines are are also commercially available from sources such as Zen-Bio (Research Triangle Park, NC), Gigacyte (Branford, CT), or Triangle Research Labs (Research Triangle Park, NC). The Cellometer® Vision Cell Analyzer (or Cellometer Gigacyte Hepatometer) incorporates image based cell counting and fluorescence detection in a compact and easy-to-use instrument.  With dual fluorescence detection capabilities, Cellometer Vision is an ideal solution for many complex cell population characterization assays, such as reliable counting and viability determination of primary hepatocytes. Reliable concentration and viability determination of primary hepatocytes is critical for accurate analysis of compound toxicity in vitro.  Due to hepatocytes’ variable morphology, fragile nature and tendency to clump, traditional manual counting methods are time-consuming and the subjectivity from operator-to-operator can cause inconsistent results. Nexcelom’s new method incorporates a ready-to-use fluorescent dual staining solution that stains live cells with acridine orange, and dead cells with propidium iodide. Researchers then load 20μL of labeled sample into a disposable counting chamber for analysis.  Because the counting chamber is disposable, no washing is required between samples and the risk of cross contamination is eliminated. Fluorescent images of the stained cells are captured and analyzed using proprietary algorithms.  Cellometer Vision’s robust operating software accurately analyzes cell images to generate live cell count, concentration and viability percentage.  Total analysis time is typically less than 60 seconds.  Cell images and all analysis data, including cell size distribution histograms, can be instantly saved for documentation.  Data can also be easily exported to Microsoft Excel spreadsheets for further analysis. Method Treat cell sample with Nexcelom’s Fluorescence Dual Staining Solution: 1. Take 20μl of freshly isolated hepatocyte sample or freeze-thaw cryopreserved cell sample in a small microtube. 2. Apply 20µl of ready-to-use dual staining solution (acridine orange/propidium iodide cocktail). 3. Gently mix.  Sample is ready to count. Running Assay: 1. Load 20µl of labeled sample into the Cellometer disposable counting chamber. 2. Insert chamber into Cellometer Vision. 3. Select assay from drop-down menu and enter Sample ID 4. Preview cell images and click ‘Count’ to begin analyzing sample. 5. Review images and counting results. 6. Save or Export images and/or report data. Results AO stained live hepatocytes are clearly visible in the fluorescent image obtained from Filter Set 101.  The software indicates counted cells with a green circle (enlarged to show detail) while ignoring cellular debris. The software can also recognize and discretely count clumpy cells. PI stained dead cells are visible in the image obtained from Filter Set 202.  The software then accurately calculates total cell count, concentration and viability (below).  By using this method, live and dead cells are clearly distinguished and automatically counted for improved accuracy.  By combining a ready to use staining solution and imaging based system results can be obtained much easier and faster compared to other methods. Download the application note.]]> 3183 0 0 0 https://nebg.biobm.com/2012/06/13/2d-gel-imager-and-analyzer-at-major-european-research-center-is-successfully-used-in-unique-drug-discovery-project/ Wed, 13 Jun 2012 13:19:47 +0000 http://www.nebiogroup.com/?p=721 protein expression signature pattern is produced and these are being used to compile a reference catalog of signature patterns. To study this protein expression, we run a large number of 1D and 2D protein gels so we need an easy to use, yet accurate imaging system.”t a UK university to help visualize protein signals which will detect how cells in the human body react to new drugs.The researcher added: “We reviewed systems from two other suppliers before we installed our G:BOX in 2008 but we were not able to achieve the level of 2D gel imaging and analysis we wanted with either system. In the time we have been using the G:BOX we have found we can easily view our blots and accurately quantify protein expression, without needing a manual and an hour to get up and running and this has helped us advance our project.”

Laura Sullivan, Syngene’s Divisional Manager, explained: “Thousands of drugs are tested each year but only a fraction are used to treat patients and this is why any new technology to reduce the time and expense of detecting those effective drugs is so important. We are delighted that our imaging system is playing a role in this project because it is proving that for high throughput analysis of 2D gels, a high resolution G:BOX system really does offer exceptional performance.” Syngene systems are sold direct in New England exclusively through New England BioGroup, LLC.

]]> 3184 0 0 0 https://nebg.biobm.com/2012/06/13/syngene-gbox-chemi-xt4-for-whole-plant-imaging-application-helps-scientists-accurately-track-and-analyze-fluorescent-proteins/ Wed, 13 Jun 2012 13:23:54 +0000 http://www.nebiogroup.com/?p=725 binding proteins are easily visualized in whole plants. Since the light tight cabinet which comes with the G:BOXXT4 system is spacious, plants including Arabidopsis will easily fit into the cabinet. To make plant imaging quick and simple, the G:BOXChemi XT4 system is controlled by Syngene’s intuitive GeneSys software, which automatically sets up the optimum imaging conditions for every fluorescently labeled protein. This means plant biologists can capture a high-quality whole plant image in seconds, with minimum training. To save time with quantifying proteins, the G:BOXChemi XT4 system also includes GeneTools image analysis software, enabling researchers to instantly determine how much of their marker proteins are being expressed. Laura Sullivan, Syngene’s Divisional Manager explained: “Many researchers want to trace their plant proteins and record the results easily but until now there hasn’t been an imaging system that could do this quantitatively. We’re delighted that our technical team has worked on this problem to determine not only the best, but also the safest imaging conditions for visualizing fluorescently labeled plant proteins. This new application means the Syngene G:BOXChemi XT4 is the best system for plant biologists wanting a simple method of pinpointing where and how much of their fluorescent proteins are being expressed in whole plants.” Syngene systems are available in New England and are sold direct and exclusively through New England BioGroup, LLC]]> 3185 0 0 0 https://nebg.biobm.com/2012/06/15/no-need-to-wear-blue-light-transilluminator-glasses-with-the-ultraslim-or-ultrabright-systems/ Fri, 15 Jun 2012 03:54:41 +0000 http://www.nebiogroup.com/?p=737 [/caption] A Blue light box offers advantages over UV transilluminators when viewing and cutting DNA gels and protein gels.  They are safer to use and do not not have the risk of a burn to eyes or skin from exposure to UV.  They also do not damage DNA, a risk inherent with the use of a UV transilluminators.  This makes the UltraSlim and UltraBright blue light transilluminators excellent choices for cutting bands from gels. Another nice feature of the UltraBright and UltraSlim blue light transilluminator is the folding variable position amber screen.  The screen can be adjusted for optimal viewing angles and the end user is able to view gel bands without the need to purchase, and wear, blue light transilluminator glasses.  The variable position screen gives researchers easy access and viewing for cutting bands from gels. New England BioGroup offers the UltraSlim and UltraBright UV transillumators at very reasonable prices.  We are available to discuss your options and help you decide which system best fits your needs.  ]]> 3186 0 0 0 https://nebg.biobm.com/2012/06/16/blue-light-transilluminator-stains/ Sat, 16 Jun 2012 14:46:51 +0000 http://www.nebiogroup.com/?p=765 UltraBright and UltraSlim blue light LED transilluminators available through New England BioGroup, and sold as an option with a Syngene gel documenation system, emit at the 470 nm wavelenght, which is safe for DNA.  Our LED transilluminators have an attached fold down, variable position amber screen.  Researchers can easily cut bands and do not need to wear cumbersome amber glasses while working with our system.

Below is a list of popular nucleic acid gel stains that can be viewed using our blue light box:

• EtBr (Ethidium Brominde)
• SYBR^® Safe
• SYBR Gold
• SYBR^® Green I & II
• SYPRO^® Ruby
• SYPRO^® Orange
• Coomassie Fluor™ Orange stains
• GelGreen,
• GelRed
• Lumitein™ Protein Gel Stain

If you are using a stain that is not on this list, please contact us to see if it will work for your application. ]]> 3187 0 0 0 https://nebg.biobm.com/2012/06/17/syngene-compact-gel-documentation-system/ Sun, 17 Jun 2012 13:32:19 +0000 http://www.nebiogroup.com/?p=782 Syngene is a world-leading manufacturer of gel documentation system.  The PXi, Syngene's newest addition to its product portfolio,  is a new high resolution, multi-application image analysis system. This powerful system is currently the best in its class for scientists that want a compact, one-click method for accurately imaging chemiluminescent and fluorescent blots, as well as 1-D gels (up to 10cm x 12 cm) stained with any type of fluorescent dye. The system sports 6.3 megapixel goodness and unparalleled sensitivity for a variety of mulitfunctional and mulitplex applications. The innovatively designed compact PXi, with its high resolution camera and large fixed aperture "fast" lens can quickly image even the faintest bands. It is easy to fit a range of lighting including IR (infrared) lighting and filter options inside the PXi system and by using Syngene’s intuitive GeneSys imaging software, the PXi can be rapidly set up to automatically select the  est conditions. Scientists can generate perfect images of chemiluminescent blots, as well as 1-D gels (up to 10cm x 12cm) stained with any fluorescence and IR commercial dyes time after time, with just one-click. GeneTools, Syngene’s accurate image analysis software, which comes with the PXi saves valuable time by automatically producing results in seconds, including; 1-D lane analysis,  molecular weight and quantity calculations. Laura Sullivan, Syngene’s Divisional Manager stated: “Syngene is well known for its imaging innovation and using the technology from our popular G:BOX, we have really pulled out all the stops by developing a unique high performance imaging system. The system is so compact, yet produces exceptional images of every type of chemi blot, as well as fluorescent blots  and gels.” New England BioGroup is excited to be able to offer this newest gel documentation system from Syngene.  Researchers in Massachusetts, Connecticut, New Hampshire, Rhode Island, Maine and Vermont will all have an opportunity to work with the latest , cutting edge gel documentation system technology.  Contact us to learn more about the PXi or other Syngene gel documentation systems.  We work closely with researchers to ensure we are helping them find a gel doc system that fits their needs and budget.]]> 3188 0 0 0 https://nebg.biobm.com/?p=3189 http://www.nebiogroup.com/?p=800 New England BioGroup is pleased to share this with you.  Enjoy this reprint from the excellent Bitesize Bio blog!

10 Commonly Broken Good Laboratory Practices

What comes to mind when you think of good laboratory practices? To many, good laboratory practices describes the best conduct while working at the bench. The laboratory is a complex environment and understanding how small, seemingly innocuous, actions can have such a huge impact on the outcome of an experiment will help you to ensure that every run of an experiment is a successful run. Think of memorable rookie research mistakes that you have made or seen others make, and you’ll have a good idea of what breaks the laws of good laboratory practice. You might also find that you break the laws of good laboratory practice when you:

1. Wear Personal Protective Equipment (PPE) sometimes, not all the time.

Chemicals are often hazardous, and you can contaminate a sample when deciding to work with bare hands. Even if you do everything humanly possible to reduce the risk, there will always be a chance of something dangerous spilling, splashing or exploding onto your skin or clothes. Wearing proper PPE ensures that in the rare event of an accident you will be protected. PPE is inexpensive, durable and can be easily replaced, so wear your PPE every time.

2. Decide not to run a control sample.

Controls serve two very important purposes. They show whether or not your chemistry worked appropriately and they serve as the basis by which you can make a definitive comparison between groups of samples. Consider running an experiment without a control. The data that is collected is 0 overall; in essence, it appears as if the experiment did not work. Did it really fail to run, or is the data really as it is? Run a control sample every time so you know.

3. Update a laboratory notebook with only abbreviated details.

Often, you will revisit a project to review data before publication, rerun the experiment for validation or compare one experiment to another. Always making complete entries helps you to make sense of what you did in the past: “See network drive for data file” is great as long as you note which file it is… “The control sample did not work” is ambiguous until you describe the type of control sample and experimental conditions. Give yourself a helping hand and be thorough with everything you write in your laboratory notebook.

4. Don’t write anything down.

Of course, it helps to be consistent with record keeping. At the end of a project, your supervisor will want to review procedures and data from beginning to end. You can only show and tell so much at a laboratory meeting, so when it comes to anything you’ve done in the lab, one good rule to live by is, “If it wasn’t written down, it probably didn’t happen.”

5. Don’t calibrate your equipment.

An uncalibrated machine can measure fantastical values. And if you calibrate it, but select the wrong measurement mode, you can run into a situation where you grossly under- or over-measure how much stuff is inside your tube. Before using any piece of equipment, take a moment to ensure that it is properly calibrated first.

6. Use tools or equipment that are “too big” for the job.

A 100ul volume can be measured with either a 100ul or 1000ul pipettor, but the exact measurement between those two pipettors will differ. Even with a 1% error, the difference in volume pipetted could be 1ul or 10ul, respectively (+/- 10X). Every instrument has its limitations. Keep variability within your experiment low by selecting instruments that are the right size for your measurements.

7. Work through your math and units only once.

Practice the habit of double- and triple-checking your work. Before mixing up that expensive batch of media, review units and calculations to see that your numbers make sense.

8. Use samples before quality checking them.

Chemical carryover of chloroform, phenol, ethanol or salts from DNA or RNA extractions can halt reactions in other experiments. Checking the integrity and quality of your samples through spectroscopy, gels or other means is a simple way to find out if you need to clean up before moving on. Strive to generate the highest quality samples that you can, above and beyond any noted lab- and assay-minimum requirements.

9. Put off required refresher training.

Yearly refresher training may seem redundant, yet it serves a very important purpose: to ensure that all staff are on the same page when it comes to safety, conduct and responsibility. This training keeps important topics fresh in your mind. Who knows, one day you may draw upon it to help a colleague to return to good laboratory practices.

10. Communicate with your lab-mates sparingly.

The road to a successfully completed project is filled with collaboration and communication. The more you communicate with those around you, the better chance you have of accommodating everyone’s needs. This is especially important when all equipment and bench space is shared. And if you work in a lab that routinely does fluorescence microscopy, it’s always nice to have a heads-up before unexpectedly finding yourself having to work in the dark. These 10 common examples show how good laboratory practices can be broken and why they are important to keep in mind while working at the bench. Many times recalibrating, remeasuring, or redoing an experiment is all that’s needed to return to best practices in the lab. What other conduct breaks good laboratory practice, and why is it noteworthy? Please share your experiences with us. Jason Erk  holds a BS in Biochemistry and is a faculty research assistant at an Oregon university. While current research efforts span the fields of behavior, neuroscience, and molecular biology, he manages copious amounts of data and freezers full of samples.]]> 3189 0 0 0 https://nebg.biobm.com/products/ Wed, 06 Mar 2024 13:19:44 +0000 https://nebg.biobm.com/?page_id=3656 /*! elementor - v3.20.0 - 26-03-2024 */ .elementor-heading-title{padding:0;margin:0;line-height:1}.elementor-widget-heading .elementor-heading-title[class*=elementor-size-]>a{color:inherit;font-size:inherit;line-height:inherit}.elementor-widget-heading .elementor-heading-title.elementor-size-small{font-size:15px}.elementor-widget-heading .elementor-heading-title.elementor-size-medium{font-size:19px}.elementor-widget-heading .elementor-heading-title.elementor-size-large{font-size:29px}.elementor-widget-heading .elementor-heading-title.elementor-size-xl{font-size:39px}.elementor-widget-heading .elementor-heading-title.elementor-size-xxl{font-size:59px}

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Crocodile 5-in-one ELISA miniWorkstation Running your ELISA assays without automation requires an hour or more of your attention, making it difficult... Details Crocodile 4-in-one Assay miniWorkstation Many assays performed in microplates involve several washing, dispensing, incubation and shaking steps.... Details Junior LB 9509 Portable Luminometer The Junior is a small portable tube luminometer which can be used for all common applications using glow... Details Lumat Luminometer for Tubes The new Lumat is a tube luminometer that uses state-of-the-art technology based on more than 40 years... Details NightOWL II LB 983 In Vivo Imaging System The NightOWL II uses an ultrasensitive back-illuminated (backlit) CCD camera with high quantum efficiency... Details NightSHADE evo In Vivo Plant Imaging System The NightSHADE evo uses an ultrasensitive back-illuminated (backlit) CCD camera with high quantum efficiency... Details QUANTOM Tx™ Microbial Cell Counter No more waiting days to count CFUs. In less than 30 seconds, the QUANTOM Tx™ scans up to 10 fields of... Details DeepLabel™ Antibody Staining Kit for Cleared Tissues DeepLabel™ Antibody Staining Kit enhances antibody penetration into large clarified tissues for vibrant... Details X-CLARITY™ Tissue Clearing Systems & Reagents The X-CLARITY™ is a collection of systems and ready-to-use reagents developed by Logos Biosystems to... Details LUNA-FX7™ Automated Cell Counter All new optics and upgraded software algorithm with high-speed and precision autofocus. Options for a... Details CELENA X|High Content Imaging System Fully automated plate and slide imaging Automated vessel handling and scanning Motorized XYZ stage, filter... Details Centro Microplate Luminometer | Microplate Reader The Centro supports all basic luminescence technologies including: Flash-type Luminescence Glow-type... Details No products found Previous 1 2 3 4 5 Next ]]> 3656 0 0 0 https://nebg.biobm.com/about-us/manufacturers-inquiries/ Wed, 24 Apr 2024 09:55:40 +0000 https://nebg.biobm.com/?page_id=4294 /*! elementor - v3.21.0 - 15-04-2024 */ .elementor-heading-title{padding:0;margin:0;line-height:1}.elementor-widget-heading .elementor-heading-title[class*=elementor-size-]>a{color:inherit;font-size:inherit;line-height:inherit}.elementor-widget-heading .elementor-heading-title.elementor-size-small{font-size:15px}.elementor-widget-heading .elementor-heading-title.elementor-size-medium{font-size:19px}.elementor-widget-heading .elementor-heading-title.elementor-size-large{font-size:29px}.elementor-widget-heading .elementor-heading-title.elementor-size-xl{font-size:39px}.elementor-widget-heading .elementor-heading-title.elementor-size-xxl{font-size:59px}

Manufacturers’ Inquiries

We appreciate your interest in working with New England BioGroup.  Please review the information below and if you believe there is a fit, contact me (Lou Farrell) via the phone number or e-mail at the bottom.

Our Territory:

NE BioGroup serves the six New England states in the US: Massachusetts, Connecticut, Rhode Island, Maine, Vermont, and New Hampshire.  Our territory is home to four of the eight Ivy League schools in the United States: Harvard, Yale, Brown and Dartmouth College.  Other preeminent academic institutions include MIT, University of Massachusetts, University of Vermont, and University of Connecticut, to name a few.  New England is also home to many major biotechnology and pharma companies: Pfizer, Bristol Myers Squibb, Novartis, Boehringer Ingelheim, Genzyme, and more.

How We Do Business:

New England BioGroup has been in business for over 10 years.  Our business model is simple: we sell great products and build long-lasting relationships with our customers.   Having been in business for many years, we know how to effectively promote and sell the products we offer in our territory by leveraging the goodwill and loyalty we have earned from our customers.  We seek to enter into relationships with companies that are willing to work with us on an exclusive basis within this territory.  Also, we greatly prefer to work with companies who value their customers the same way we do and genuinely look to help them, rather than simply viewing them as a sale.

At New England Biogroup

For Manufacturers

New England BioGroup has been providing professional sales representation to global brands for 30 years. If you have innovative, high-quality life science instruments and would like us to represent you, please provide your email below and we will be in contact.

]]> 4294 2856 0 0 We appreciate your interest in working with New England BioGroup. \u00a0Please review the\u00a0information\u00a0below and if you believe there is a fit, contact me (Lou Farrell) via the phone number or e-mail at the bottom.<\/p>","align":"center","__globals__":{"text_color":""},"text_color":"#000000"},"elements":[],"widgetType":"text-editor"}],"isInner":false}],"isInner":false},{"id":"1d3b8e1","elType":"container","settings":{"flex_direction":"column","background_color":"#4A456C","background_color_b":"#FDFDFD","background_color_b_stop":{"unit":"%","size":21,"sizes":[]},"background_overlay_hover_transition":{"unit":"px","size":0.3,"sizes":[]},"uc_background_webgl_slider_custom_displacement_image":{"id":2942,"url":"https:\/\/nebg.biobm.com\/wp-content\/uploads\/2024\/02\/placeholder-35.png"},"uc_background_webgl_slider_uc_items":[{"title":"Slide 1","slide_image":{"id":2943,"url":"https:\/\/nebg.biobm.com\/wp-content\/uploads\/2024\/02\/dynamic-wang-F3KHLtv6844-unsplash-34.webp"},"slide_image_attributes_nosize":" src=\"https:\/\/nebg.biobm.com\/wp-content\/uploads\/ac_assets\/background_webgl_slider\/images\/dynamic-wang-F3KHLtv6844-unsplash.webp\"","slide_image_attributes":" src=\"https:\/\/nebg.biobm.com\/wp-content\/uploads\/ac_assets\/background_webgl_slider\/images\/dynamic-wang-F3KHLtv6844-unsplash.webp\"","slide_image_thumb":"https:\/\/nebg.biobm.com\/wp-content\/uploads\/ac_assets\/background_webgl_slider\/images\/dynamic-wang-F3KHLtv6844-unsplash.webp","item_repeater_class":"elementor-repeater-item-rfmom","_id":"93fa85a"},{"title":"Slide 2","slide_image":{"id":2944,"url":"https:\/\/nebg.biobm.com\/wp-content\/uploads\/2024\/02\/dynamic-wang-yZlq7BDPLfQ-unsplash-34.webp"},"slide_image_attributes_nosize":" src=\"https:\/\/nebg.biobm.com\/wp-content\/uploads\/ac_assets\/background_webgl_slider\/images\/dynamic-wang-yZlq7BDPLfQ-unsplash.webp\"","slide_image_attributes":" src=\"https:\/\/nebg.biobm.com\/wp-content\/uploads\/ac_assets\/background_webgl_slider\/images\/dynamic-wang-yZlq7BDPLfQ-unsplash.webp\"","slide_image_thumb":"https:\/\/nebg.biobm.com\/wp-content\/uploads\/ac_assets\/background_webgl_slider\/images\/dynamic-wang-yZlq7BDPLfQ-unsplash.webp","item_repeater_class":"elementor-repeater-item-b5gwn","_id":"852795f"},{"title":"Slide 3","slide_image":{"id":2945,"url":"https:\/\/nebg.biobm.com\/wp-content\/uploads\/2024\/02\/dynamic-wang-ltRRbmk7eR4-unsplash_2-34.webp"},"slide_image_attributes_nosize":" src=\"https:\/\/nebg.biobm.com\/wp-content\/uploads\/ac_assets\/background_webgl_slider\/images\/dynamic-wang-ltRRbmk7eR4-unsplash_2.webp\"","slide_image_attributes":" src=\"https:\/\/nebg.biobm.com\/wp-content\/uploads\/ac_assets\/background_webgl_slider\/images\/dynamic-wang-ltRRbmk7eR4-unsplash_2.webp\"","slide_image_thumb":"https:\/\/nebg.biobm.com\/wp-content\/uploads\/ac_assets\/background_webgl_slider\/images\/dynamic-wang-ltRRbmk7eR4-unsplash_2.webp","item_repeater_class":"elementor-repeater-item-v3b9q","_id":"314f804"},{"title":"Slide 4","slide_image":{"id":2946,"url":"https:\/\/nebg.biobm.com\/wp-content\/uploads\/2024\/02\/dynamic-wang-ltRRbmk7eR4-unsplash_1-34.webp"},"slide_image_attributes_nosize":" src=\"https:\/\/nebg.biobm.com\/wp-content\/uploads\/ac_assets\/background_webgl_slider\/images\/dynamic-wang-ltRRbmk7eR4-unsplash_1.webp\"","slide_image_attributes":" src=\"https:\/\/nebg.biobm.com\/wp-content\/uploads\/ac_assets\/background_webgl_slider\/images\/dynamic-wang-ltRRbmk7eR4-unsplash_1.webp\"","slide_image_thumb":"https:\/\/nebg.biobm.com\/wp-content\/uploads\/ac_assets\/background_webgl_slider\/images\/dynamic-wang-ltRRbmk7eR4-unsplash_1.webp","item_repeater_class":"elementor-repeater-item-qy8lv","_id":"7aa2419"}],"padding":{"unit":"px","top":"75","right":"80","bottom":"0","left":"80","isLinked":false},"padding_tablet":{"unit":"px","top":"50","right":"30","bottom":"0","left":"30","isLinked":false},"padding_mobile":{"unit":"px","top":"30","right":"10","bottom":"30","left":"10","isLinked":false}},"elements":[{"id":"a64f7c0","elType":"widget","settings":{"title":"Our Territory:\n","title_color":"#232323","__globals__":{"typography_typography":"globals\/typography?id=fac0cc3"}},"elements":[],"widgetType":"heading"},{"id":"bc9ce80","elType":"widget","settings":{"editor":"

NE BioGroup serves the six New England states in the US: Massachusetts, Connecticut, Rhode Island, Maine, Vermont, and New Hampshire. \u00a0Our territory is home to four of the eight Ivy League schools in the United States: Harvard, Yale, Brown and Dartmouth College. \u00a0Other preeminent academic institutions include MIT, University of Massachusetts, University of Vermont, and University of Connecticut, to name a few. \u00a0New England is also home to many major biotechnology and pharma companies: Pfizer, Bristol Myers Squibb, Novartis, Boehringer Ingelheim, Genzyme, and more.<\/p>","align":"left","__globals__":{"text_color":"globals\/colors?id=b8d1d84","typography_typography":""},"align_mobile":"center","typography_typography":"custom","typography_font_family":"Poppins","typography_font_size":{"unit":"px","size":16,"sizes":[]},"typography_font_size_tablet":{"unit":"px","size":14,"sizes":[]},"typography_font_size_mobile":{"unit":"px","size":14,"sizes":[]},"typography_font_weight":"400","typography_text_transform":"none","typography_font_style":"normal","typography_text_decoration":"none","typography_line_height":{"unit":"px","size":28,"sizes":[]},"typography_line_height_tablet":{"unit":"px","size":"","sizes":[]},"typography_line_height_mobile":{"unit":"px","size":24,"sizes":[]}},"elements":[],"widgetType":"text-editor"},{"id":"cabedf4","elType":"widget","settings":{"title":"How We Do Business:\n","title_color":"#232323","__globals__":{"typography_typography":"globals\/typography?id=fac0cc3"}},"elements":[],"widgetType":"heading"},{"id":"c53b298","elType":"widget","settings":{"editor":"

New England BioGroup has been in business for over 10 years. \u00a0Our business model is simple: we sell great products and build long-lasting relationships with our customers. \u00a0 Having been in business for many years, we know how to effectively promote and sell the products we offer in our territory by leveraging the goodwill and loyalty we have earned from our customers. \u00a0We seek to enter into relationships with companies that are willing to work with us on an exclusive basis within this territory. \u00a0Also, we greatly prefer to work with companies who value their customers the same way we do and genuinely look to help them, rather than simply viewing them as a 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Events

Mark your calendar! New England BioGroup actively participates in local vendor shows. Stop by our table to chat and take a look at our featured products. Pick up a free pen, enter our raffle (if permitted), and grab some chocolates…we give out the good stuff!. If you need more info than what you see below, contact us.

At New England Biogroup

For Manufacturers

New England BioGroup has been providing professional sales representation to global brands for 30 years. If you have innovative, high-quality life science instruments and would like us to represent you, please provide your email below and we will be in contact.

]]> 4309 0 0 0 New England BioGroup has been providing professional sales representation to global brands for 30 years. If you have innovative, high-quality life science instruments and would like us to represent you, please provide your email below and we will be in contact.<\/p>","align_mobile":"center","__globals__":{"text_color":"globals\/colors?id=b8d1d84","typography_typography":""},"align":"center","_margin":{"unit":"px","top":"10","right":"0","bottom":"40","left":"0","isLinked":false},"text_color":"#232323","typography_typography":"custom","typography_font_family":"Poppins","typography_font_size":{"unit":"px","size":16,"sizes":[]},"typography_font_size_tablet":{"unit":"px","size":16,"sizes":[]},"typography_font_size_mobile":{"unit":"px","size":16,"sizes":[]},"typography_font_weight":"300","typography_text_transform":"none","typography_font_style":"normal","typography_text_decoration":"none","typography_line_height":{"unit":"px","size":22,"sizes":[]},"typography_line_height_tablet":{"unit":"px","size":"","sizes":[]},"typography_line_height_mobile":{"unit":"px","size":"","sizes":[]},"_margin_mobile":{"unit":"px","top":"0","right":"0","bottom":"30","left":"0","isLinked":false},"_element_width":"initial","_element_custom_width":{"unit":"%","size":90,"sizes":[]},"_element_custom_width_mobile":{"unit":"%","size":100,"sizes":[]}},"elements":[],"widgetType":"text-editor"},{"id":"90ce06c","elType":"widget","settings":{"form_name":"New Form","form_fields":[{"custom_id":"field_bcacc37","field_type":"email","required":"true","field_label":"Email","placeholder":"Your Email Address","width":"80","width_mobile":"80","_id":"bcacc37","file_sizes":"","file_types":"","allow_multiple_upload":"","max_files":"","acceptance_text":"","checked_by_default":"","field_min":"","field_max":"","min_date":"","max_date":"","use_native_date":"","use_native_time":"","previous_button":"","next_button":""}],"show_labels":"","button_text":"","button_width":"20","selected_button_icon":{"value":"fas fa-long-arrow-alt-right","library":"fa-solid"},"button_icon_align":"right","email_content":"[all-fields]","email_content_2":"[all-fields]","success_message":"The form was sent successfully.","error_message":"An error occured.","required_field_message":"This field is required.","invalid_message":"There's something wrong. The form is invalid.","button_text_padding":{"unit":"px","top":"10","right":"10","bottom":"10","left":"10","isLinked":false},"button_width_mobile":"20","button_typography_typography":"custom","button_typography_font_size":{"unit":"px","size":25,"sizes":[]},"column_gap":{"unit":"px","size":0,"sizes":[]},"input_size":"md","step_next_label":"Next","step_previous_label":"Previous","__globals__":{"button_background_color":"","field_text_color":"","button_background_hover_color":"","button_border_color":"","button_hover_border_color":""},"button_icon_indent":{"unit":"px","size":0,"sizes":[]},"html_spacing":{"unit":"px","size":"","sizes":[]},"button_background_color":"#4A456C","button_background_hover_color":"#2E75CF","_margin":{"unit":"%","top":"0","right":"10","bottom":"0","left":"10","isLinked":false},"_margin_mobile":{"unit":"px","top":"0","right":"0","bottom":"0","left":"0","isLinked":false},"button_typography_font_size_mobile":{"unit":"px","size":20,"sizes":[]},"field_border_width":{"unit":"px","top":"1","right":"1","bottom":"1","left":"1","isLinked":true},"button_border_border":"solid","button_border_width":{"unit":"px","top":"1","right":"1","bottom":"1","left":"1","isLinked":true},"button_border_width_mobile":{"unit":"px","top":"1","right":"1","bottom":"1","left":"1","isLinked":true},"mailchimp_fields_map":[],"drip_fields_map":[],"activecampaign_fields_map":[],"getresponse_fields_map":[],"convertkit_fields_map":[],"mailerlite_fields_map":[],"email_to":"furqan@biobm.com","email_subject":"New message from "New England BioGroup"","email_from":"email@nebg.biobm.com","email_from_name":"New England BioGroup","email_to_2":"furqan@biobm.com","email_subject_2":"New message from "New England BioGroup"","email_from_2":"email@nebg.biobm.com","email_from_name_2":"New England BioGroup","email_reply_to_2":"furqan@biobm.com","server_message":"Your submission failed because of a server error.","button_border_color":"#4A456C","previous_button_background_color":"#4A456C","previous_button_border_color":"#4A456C","button_hover_border_color":"#2E75CF","previous_button_background_color_hover":"#2E75CF","previous_button_border_color_hover":"#2E75CF","field_border_radius":{"unit":"px","top":"4","right":"0","bottom":"0","left":"4","isLinked":false},"button_border_radius":{"unit":"px","top":"0","right":"4","bottom":"4","left":"0","isLinked":false}},"elements":[],"widgetType":"form"}],"isInner":false}],"isInner":false}]]]> https://nebg.biobm.com/cell-counters/ Wed, 24 Apr 2024 10:22:34 +0000 https://nebg.biobm.com/?page_id=4324

Cell Counters

Automated cell counters enable labs to easily and accurately count many different types of cells. New England Biogroup offers Nexcelom cell counters because of their good feature set, their accuracy, and their reliability. Thousands of customers, including the top ten pharmaceutical companies, have come to rely on Cellometer instruments for automated cell concentration and viability analysis. A Cellometer cell counter provides fast and accurate results, saves time and is maintenance-free.

The Nexcelom line consists of 4 cell counters:

• Cellometer T4 – Brightfield excitation for automated cell counting a viability determination using Trypan blue stain.  Useful for most cell lines and cultured primary cells.
• Cellometer X4 – Single channel fluorescence and brightfield excitation with 10x magnification range.  Useful for counting yeast and algae.
• Cellometer Auto 2000 – Dual channel fluorescence and brightfield excitation.  Best for analysis of nucleated cells and primary cells (PBMC’s, spleenocytes, lymphocytes) from fresh samples (whole blood, cord blood, bone marrow).
• Cellometer Vision – For cell-based assays: apoptosis, cell cycle, transfection, autophagy, ABC transporter assays, and many others.  Also useful for analysis of primary cells: hepatocytes, adipocytes.

Alternatively, click the image below for more information on all of our automated cell counters.

View All Cell Counters

Why New England BioGroup?

Call us old-school, but we still believe in putting the customer before the sale. We want to help you select and purchase the product that is the best for your lab’s needs, not for our revenues. We’re also local to New England, which means not only are you supporting a local, family-owned business, but we’re right around the corner to help provide you with support when you need it. At NE BioGroup, we carefully select what products we distribute – we only work with reputable companies that make reliable, high-quality products and care about our customers just like we do. We also don’t sell everything under the sun, but maintain a smaller portfolio of specialized products and take pride in our knowledge of those areas. Feel free to ask us a question – you’ll be surprised how well we know our products (and competing products!)]]> 4324 0 0 0 \n

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Automated cell counters enable labs to easily and accurately count many different types of cells. New England Biogroup offers Nexcelom cell counters because of their good feature set, their accuracy, and their reliability. Thousands of customers, including the top ten pharmaceutical companies, have come to rely on Cellometer instruments for automated cell concentration and viability analysis. A Cellometer cell counter provides fast and accurate results, saves time and is maintenance-free.<\/p>\n

The Nexcelom line consists of 4 cell counters:<\/p>\n

\u2022\u00a0Cellometer T4<\/a><\/strong>\u00a0\u2013 Brightfield excitation for automated cell counting a viability determination using Trypan blue stain. \u00a0Useful for most cell lines and cultured primary cells<\/strong>.
\u2022\u00a0Cellometer X4<\/a><\/strong>\u00a0\u2013 Single channel fluorescence and brightfield\u00a0excitation with 10x magnification range. \u00a0Useful for counting yeast and algae<\/strong>.
\u2022\u00a0Cellometer Auto 2000<\/a><\/strong>\u00a0\u2013 Dual channel fluorescence and brightfield excitation. \u00a0Best for analysis of nucleated cells and primary cells<\/strong>\u00a0(PBMC\u2019s, spleenocytes, lymphocytes) from fresh samples (whole blood, cord blood, bone marrow).
\u2022\u00a0Cellometer Vision<\/a><\/strong>\u00a0\u2013\u00a0For cell-based assays<\/strong>: apoptosis, cell cycle, transfection, autophagy, ABC transporter assays, and many others. \u00a0Also useful for analysis of primary cells: hepatocytes, adipocytes.<\/p>\n

Alternatively, click the image below for more information on all of our automated cell counters.<\/p>","align":"left","__globals__":{"text_color":"globals\/colors?id=b8d1d84","typography_typography":""},"align_mobile":"center","typography_typography":"custom","typography_font_family":"Poppins","typography_font_size":{"unit":"px","size":16,"sizes":[]},"typography_font_size_tablet":{"unit":"px","size":14,"sizes":[]},"typography_font_size_mobile":{"unit":"px","size":14,"sizes":[]},"typography_font_weight":"400","typography_text_transform":"none","typography_font_style":"normal","typography_text_decoration":"none","typography_line_height":{"unit":"px","size":28,"sizes":[]},"typography_line_height_tablet":{"unit":"px","size":"","sizes":[]},"typography_line_height_mobile":{"unit":"px","size":24,"sizes":[]}},"elements":[],"widgetType":"text-editor"},{"id":"acf5ff2","elType":"widget","settings":{"text":"View All Cell Counters","selected_icon":{"value":"fas fa-long-arrow-alt-right","library":"fa-solid"},"icon_align":"right","icon_indent":{"unit":"px","size":10,"sizes":[]},"align_mobile":"center","button_text_color":"#FFFFFF","background_color":"#4A456C","hover_color":"#FFFFFF","button_background_hover_color":"#2E76CF","typography_typography":"custom","typography_font_family":"Poppins","typography_font_size":{"unit":"px","size":14,"sizes":[]},"typography_font_weight":"500","typography_line_height":{"unit":"px","size":20,"sizes":[]},"border_radius":{"unit":"px","top":"4","right":"4","bottom":"4","left":"4","isLinked":true},"text_padding":{"unit":"px","top":"12","right":"24","bottom":"12","left":"24","isLinked":false},"link":{"url":"\/product-category\/automated-cell-counter\/","is_external":"","nofollow":"","custom_attributes":""}},"elements":[],"widgetType":"button"},{"id":"661462b","elType":"widget","settings":{"title":"Why New England BioGroup?\n","title_color":"#232323","__globals__":{"typography_typography":"globals\/typography?id=fac0cc3"}},"elements":[],"widgetType":"heading"},{"id":"69e5dc1","elType":"widget","settings":{"editor":"Call us old-school, but we still believe in putting the customer before the sale. We want to help you select and purchase the product that is the best for your lab\u2019s needs, not for our revenues. We\u2019re also local to New England, which means not only are you supporting a local, family-owned business, but we\u2019re right around the corner to help provide you with support when you need it. At NE BioGroup, we carefully select what products we distribute \u2013 we only work with reputable companies that make reliable, high-quality products and care about our customers just like we do. We also don\u2019t sell everything under the sun, but maintain a smaller portfolio of specialized products and take pride in our knowledge of those areas. Feel free to ask us a question \u2013 you\u2019ll be surprised how well we know our products (and competing products!)","align":"left","__globals__":{"text_color":"globals\/colors?id=b8d1d84","typography_typography":""},"align_mobile":"center","typography_typography":"custom","typography_font_family":"Poppins","typography_font_size":{"unit":"px","size":16,"sizes":[]},"typography_font_size_tablet":{"unit":"px","size":14,"sizes":[]},"typography_font_size_mobile":{"unit":"px","size":14,"sizes":[]},"typography_font_weight":"400","typography_text_transform":"none","typography_font_style":"normal","typography_text_decoration":"none","typography_line_height":{"unit":"px","size":28,"sizes":[]},"typography_line_height_tablet":{"unit":"px","size":"","sizes":[]},"typography_line_height_mobile":{"unit":"px","size":24,"sizes":[]}},"elements":[],"widgetType":"text-editor"}],"isInner":false}]]]> https://nebg.biobm.com/biobm/ Thu, 25 Apr 2024 10:41:08 +0000 https://nebg.biobm.com/?page_id=4333 4333 0 0 0 https://nebg.biobm.com/?post_type=product&p=1279 Wed, 21 Feb 2024 09:33:38 +0000 https://nebg.biobm.com/?post_type=product&p=1279 SlimWhite White Light Box (Part number: NEB-SW01)

One year warranty and free shipping!

The SlimWhite white light pad is a very cost effective product for viewing colorimetric gels.   The SlimWhite offers a very large  17 cm x 27.5cm viewing area and is ideal for viewingCoomassie-stained gels, silver stained gels, or other colorimetric gels.  The SlimWhite is very compact, convenient and safe to use.  Its light source is LED and  the lifetime bulb expectancy is 50,000 hours and power consumption is ~5W. Specifications: Wavelength:  White light Light Source: LED Dimensions (cm): 21 x 30.5 x 1.5 Illumination area (cm): 17 x 27.5 Weight (kg): 1.3 Power: 12 VDC, 0.5A (optional battery box available) ]]> The SlimWhite white light pad is a very cost effective product for viewing colorimetric gels.   The SlimWhite offers a very large  17 cm x 27.5cm viewing area and is ideal for viewingCoomassie-stained gels, silver stained gels, or other colorimetric gels. Specifications: Wavelength:  White light Light Source: LED Dimensions (cm): 21 x 30.5 x 1.5 Illumination area (cm): 17 x 27.5 Weight (kg): 1.3 Power: 12 VDC, 0.5A (optional battery box available)]]> 1279 0 94 0 https://nebg.biobm.com/?post_type=product&p=1280 Wed, 21 Feb 2024 09:39:55 +0000 https://nebg.biobm.com/?post_type=product&p=1280 transilluminator for mini-gels.

An affordable system for mini-gels!

PART NUMBER: NEB-UUV-01 The UltraSlim UV transilluminator represents a great value for the lab. It is equipped with a new-design UV blocking cover which is easily detachable for access to the work surface. A UV transilluminator is optimal for researchers working with nucleic acid stains such as Ethidium Bromide and GelRedTM. It can also be used with SYBR SafeTM, GelGreenTM, or MaestrosafeTM stain. However, we suggest a blue light transilluminator for those stains.     Specifications:

• Dimensions (cm): 24.7D x 13.2W x 4.85H
• Viewing surface (cm): 15.0D x 8.0W
• Wavelength (nm): 302
• UV tubes: T5 6W UVB
• Power: 12V - 1.5A
• Weight (Kg): 1.4
• Compatible gel type: mini gels
• Compatible gel size (mm): 50*60, 105*60, 125*60
• Temperature: operation 0~40 ℃, storage -10~70 ℃
• Humidity: operation 20~80 %, storage 10~90 %

]]> Specifications:

• Dimensions (cm): 24.7D x 13.2W x 4.85H
• Viewing surface (cm): 15.0D x 8.0W
• Wavelength (nm): 302
• UV tubes: T5 6W UVB
• Power: 12V - 1.5A
• Weight (Kg): 1.4
• Compatible gel type: mini gels
• Compatible gel size (mm): 50*60, 105*60, 125*60
• Temperature: operation 0~40 ℃, storage -10~70 ℃
• Humidity: operation 20~80 %, storage 10~90 %

]]> 1280 0 88 0 https://nebg.biobm.com/?post_type=product&p=1650 Thu, 22 Feb 2024 12:40:28 +0000 https://nebg.biobm.com/?post_type=product&p=1650 UltraBright UV (Part number: NEB-MLB-21) UV transilluminator is the perfect complement to your gel electrophoresis apparatus system!  

The UltraBright UV carries a one-year warranty.  Free shipping to anywhere in the USA.

UV transilluminators are a useful and necessary tool for the molecular biology lab.  A gels that has been run on a electrophoresis system or apparatus and has been stained with a fluorescent dye such as Ethidium Bromide, SYBR Safe, SYBR Green, Gel Green, etc. will need to be placed on a UV transilluminator or blue light transilluminator for visualization and band cutting. We are proud to offer the Maestrogen UV transilluminators, which represent the a great value and price for the life science researcher.  All UltraBright UV transilluminators carry a two year warranty.  The UltraBright UV transilluminators are equipped with a new-design variable position UV blocking cover, adjustable, to different angles for access to the work surface.

Key Features

• Uniform lighting design/Ultra high contrast
• UltraSafe UV blocking cover: block 99.99% UV transmittance
• Strong frame and stylish (no breakage of UV shield)
• Fast turn on speeds for UV lamps
• Dual UV wavelengths: 302nm/365nm
• Adjustable intensity: from 100% down to 50%

Product Specifications

•  Dimensions (cm): 28D x 34W x 8H
•  Viewing surface (cm): 21D x 26W
•  Wavelength (nm): 302 & 365
•  UV tubes: 11 x 8W
•  UV blocking cover: Aluminum alloy frame
•  Power: 100-240V, 50-60Hz
•  Weight (Kg): 4.3

]]> Product Specifications

•  Dimensions (cm): 28D x 34W x 8H
•  Viewing surface (cm): 21D x 26W
•  Wavelength (nm): 302 & 365
•  UV tubes: 11 x 8W
•  UV blocking cover: Aluminum alloy frame
•  Power: 100-240V, 50-60Hz
•  Weight (Kg): 4.3

]]> 1650 0 93 0 https://nebg.biobm.com/?post_type=product&p=1662 Fri, 23 Feb 2024 08:29:51 +0000 https://nebg.biobm.com/?post_type=product&p=1662 FREE SHIPPING!

UltraBright UV MB-16 UV transilluminators are the perfect complement to your gel electrophoresis apparatus system!

UV transilluminators are a useful and necessary tool for the molecular biology lab.  A gels that has been run on a electrophoresis system or apparatus and has been stained with a fluorescent dye such as Ethidium Bromide, SYBR Safe, SYBR Green, Gel Green, etc. will need to be placed on a UV transilluminator or blue light transilluminator for visualization and band cutting. We are proud to offer the Maestrogen UV transilluminators, which represent the a great value and price for the life science researcher.  All UltraBright UV transilluminators carry a one year warranty.  The UltraBright UV transilluminators are equipped with a new-design variable position UV blocking cover, adjustable, to different angles for access to the work surface.

Key Features

• Uniform lighting design
• UltraSafe UV blocking cover: block 99.99% UV transmittance
• UV wavelength: 302nm
• Adjustable intensity: from 100% down to 50%

Product Specifications

•  Dimensions(cm): 28D x 34W x 8H
•  Viewing surface(cm): 16D x 20W
•  Wavelength(nm): 302
•  UV tubes: 6 x 8W
•  UV blocking cover: Aluminum alloy frame
•  Power: 100-240V, 50-60Hz
•  Weight (Kg): 4.3

]]> Product Specifications

•  Dimensions(cm): 28D x 34W x 8H
•  Viewing surface(cm): 16D x 20W
•  Wavelength(nm): 302
•  UV tubes: 6 x 8W
•  UV blocking cover: Aluminum alloy frame
•  Power: 100-240V, 50-60Hz
•  Weight (Kg): 4.3

]]> 1662 0 89 0 https://nebg.biobm.com/?post_type=product&p=1664 Fri, 23 Feb 2024 08:31:50 +0000 https://nebg.biobm.com/?post_type=product&p=1664 FREE SHIPPING!

UltraBright UV MLB-16 UV transilluminator is the perfect complement to your gel electrophoresis apparatus system!

UV transilluminators are a useful and necessary tool for the molecular biology lab.  A gels that has been run on a electrophoresis system or apparatus and has been stained with a fluorescent dye such as Ethidium Bromide, SYBR Safe, SYBR Green, Gel Green, etc. will need to be placed on a UV transilluminator or blue light transilluminator for visualization and band cutting. We are proud to offer the Maestrogen UV transilluminators, which represent the a great value and price for the life science researcher.  All UltraBright UV transilluminators carry a one year warranty.  The UltraBright UV transilluminators are equipped with a new-design variable position UV blocking cover, adjustable, to different angles for access to the work surface.

Key Features

• Uniform lighting design/Ultra high contrast
• UltraSafe UV blocking cover: block 99.99% UV transmittance
• Strong frame and stylish (no breakage of UV shield)
• Fast turn on speeds for UV lamps
• Dual UV wavelengths: 302nm/365nm
• Adjustable intensity: from 100% down to 50%

Product Specifications

•  Dimensions (cm): 28D x 34W x 8H
•  Viewing surface (cm): 16D x 20W
•  Wavelength (nm): 302 & 365
•  UV tubes: 9 x 8W
•  UV blocking cover: Aluminum alloy frame
•  Power: 100-240V, 50-60Hz
•  Weight (Kg): 4.3

]]> Product Specifications

•  Dimensions (cm): 28D x 34W x 8H
•  Viewing surface (cm): 16D x 20W
•  Wavelength (nm): 302 & 365
•  UV tubes: 9 x 8W
•  UV blocking cover: Aluminum alloy frame
•  Power: 100-240V, 50-60Hz
•  Weight (Kg): 4.3

]]> 1664 0 90 0 https://nebg.biobm.com/?post_type=product&p=1665 Fri, 23 Feb 2024 08:33:36 +0000 https://nebg.biobm.com/?post_type=product&p=1665 FREE SHIPPING!

UltraBright UV MB-20 UV transilluminators are the perfect complement to your gel electrophoresis apparatus system!

UV transilluminators are a useful and necessary tool for the molecular biology lab.  A gels that has been run on a electrophoresis system or apparatus and has been stained with a fluorescent dye such as Ethidium Bromide, SYBR Safe, SYBR Green, Gel Green, etc. will need to be placed on a UV transilluminator or blue light transilluminator for visualization and band cutting. We are proud to offer the Maestrogen UV transilluminators, which represent the a great value and price for the life science researcher.  All UltraBright UV transilluminators carry a two year warranty.  The UltraBright UV transilluminators are equipped with a new-design variable position UV blocking cover, adjustable, to different angles for access to the work surface.

Key Features

• Uniform lighting design
• UltraSafe UV blocking cover: block 99.99% UV transmittance
• UV wavelength: 302nm
• Adjustable intensity: from 100% down to 50%

Product Specifications

•  Dimensions(cm): 28D x 34W x 8H
•  Viewing surface(cm): 20D x 20W
•  Wavelength(nm): 302
•  UV tubes: 6 x 8W
•  UV blocking cover: Aluminum alloy frame
•  Power: 100-240V, 50-60Hz
•  Weight (Kg): 4.3

]]> Product Specifications

•  Dimensions(cm): 28D x 34W x 8H
•  Viewing surface(cm): 20D x 20W
•  Wavelength(nm): 302
•  UV tubes: 6 x 8W
•  UV blocking cover: Aluminum alloy frame
•  Power: 100-240V, 50-60Hz
•  Weight (Kg): 4.3

]]> 1665 0 91 0 https://nebg.biobm.com/?post_type=product&p=1666 Fri, 23 Feb 2024 08:35:41 +0000 https://nebg.biobm.com/?post_type=product&p=1666 FREE SHIPPING!

UltraBright UV MB-21 UV transilluminator is the perfect complement to your gel electrophoresis apparatus system!

UV transilluminators are a useful and necessary tool for the molecular biology lab.  A gels that has been run on a electrophoresis system or apparatus and has been stained with a fluorescent dye such as Ethidium Bromide, SYBR Safe, SYBR Green, Gel Green, etc. will need to be placed on a UV transilluminator or blue light transilluminator for visualization and band cutting. We are proud to offer the Maestrogen UV transilluminators, which represent the a great value and price for the life science researcher.  All UltraBright UV transilluminators carry a one year warranty.  The UltraBright UV transilluminators are equipped with a new-design variable position UV blocking cover, adjustable, to different angles for access to the work surface.

Key Features

• Uniform lighting design/Ultra high contrast
• UltraSafe UV blocking cover: block 99.99% UV transmittance
• Strong frame and stylish (no breakage of UV shield)
• Fast turn on speeds for UV lamps
• Adjustable intensity: from 100% down to 50%

Product Specifications

• Dimensions(cm): 28D x 34W x 8H
•  Viewing surface(cm): 21D x 26W
•  Wavelength(nm): 302
•  UV tubes: 6 x 8W
•  UV blocking cover: Aluminum alloy frame
•  Power: 100-240V, 50-60Hz
•  Weight (Kg): 4.3

]]> Product Specifications

• Dimensions(cm): 28D x 34W x 8H
•  Viewing surface(cm): 21D x 26W
•  Wavelength(nm): 302
•  UV tubes: 6 x 8W
•  UV blocking cover: Aluminum alloy frame
•  Power: 100-240V, 50-60Hz
•  Weight (Kg): 4.3

]]> 1666 0 92 0 https://nebg.biobm.com/?post_type=product&p=1667 Fri, 23 Feb 2024 08:42:46 +0000 https://nebg.biobm.com/?post_type=product&p=1667 Galileo Reflection™ 2010 Dual-Gel Vertical Electrophoresis Unit by Galileo bioscience Run either one or two gels simultaneously on these versatile separation systems.  Similar to the 1010 unit but designed to run one or two larger format 20cm wide x 10cm tall gels. Unique casting base allows both casting and running of the gel without unclamping from the upper buffer chamber, providing for safer and more convenient gel handling. Water cooling feature is included for protocols that require it. Connections are easily made by attaching standard tubing over the 3/8" barbed in/out ports provided on the device. Water may be provided by tap or other temperature controlled water source. Optional alumina backer plates may be used to facilitate cooling efficiency and uniformity, and is shown in the accessories list below.

System Features

The GalileoReflection™ 85-2010-V complete system includes these features:

• Heavy duty upper buffer chamber with cooling port connections.
• Lower buffer chamber.
• Interlock safety lid with attached leads.
• Casting base.
• Two-pack each of plain & notched glass plates
• One acrylic blocker plate (used when running only one gel)
• Two 15-well, 0.8mm thick combs.
• Two 20-well, 0.8mm thick combs.
• Eight 0.8mm thick side spacers

System accessories

Choose amoung these accessories to customize each system for your specific needs.

• Alumina cooling plate. For use with cooling systems. This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
• Casting base. For quick, efficient and leak-proof casting.

View the Galileo Bioscience electrophoresis and blotting system catalog]]> System accessories Choose amoung these accessories to customize each system for your specific needs.

• Alumina cooling plate. For use with cooling systems. This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
• Casting base. For quick, efficient and leak-proof casting.

]]> 1667 0 75 0 https://nebg.biobm.com/?post_type=product&p=1669 Fri, 23 Feb 2024 08:43:21 +0000 https://nebg.biobm.com/?post_type=product&p=1669  Galileo Reflection™ 2020 Dual-Gel  Vertical Electrophoresis Unit by Galileo bioscience Run either one or two extra wide long-run gels simultaneously on these versatile separation systems.  The 2020 unit is designed to provide the researcher with both high cample capacity and increased run length. Runs either one or two larger format 20cm wide x 20cm tall gels. Unique casting base allows both casting and running of the gel without unclamping from the upper buffer chamber, providing for safer and more convenient gel handling. Water cooling feature is included for protocols that require it. Connections are easily made by attaching standard tubing over the 3/8" barbed in/out ports provided on the device. Water may be provided by tap or other temperature controlled water source. Optional alumina backer plates may be used to facilitate cooling efficiency and uniformity, and is shown in the accessories list below.

System Features

The Galileo Reflection™ 85-2020 complete system includes these features:

• Heavy duty upper buffer chamber with cooling port connections.
• Lower buffer chamber.
• Interlock safety lid with attached leads.
• Casting base.
• Four-pack each of plain & notched glass plates
• One acrylic blocker plate (used when running only one gel)
• Two 15-well, 1.5mm thick combs.
• Two 20-well, 1.5mm thick combs.
• Eight 1.5mm thick side spacers

System accessories

Choose amoung these accessories to customize each system for your specific needs.

• Alumina cooling plate. For use with cooling systems. This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
• Casting base. For quick, efficient and leak-proof casting.

View the Galileo Bioscience electrophoresis and blotting system catalog]]> System accessories Choose amoung these accessories to customize each system for your specific needs.

• Alumina cooling plate. For use with cooling systems. This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
• Casting base. For quick, efficient and leak-proof casting.

]]> 1669 0 76 0 https://nebg.biobm.com/?post_type=product&p=1688 Fri, 23 Feb 2024 11:03:31 +0000 https://nebg.biobm.com/?post_type=product&p=1688 1688 0 61 0 https://nebg.biobm.com/2012/06/28/auto-2000-counts-mononuclear-cells/ Thu, 28 Jun 2012 02:09:23 +0000 http://www.nebiogroup.com/?p=819 An Alternative to Flow Cytometry!  Cellometer Auto 2000 Offers Accurate and Reproducible Counting Of Peripheral Blood Mononuclear Cells (PBMC). Peripheral blood mononuclear cells are used to measure immunological functions. Assays include proliferation, cytotoxicity and cytokine production. The use of cryopreserved PBMCs is critical both in clinical trials and biomedical research. In cancer immunotherapy, leukapheresis is often used to isolate immune system cells from a patient's blood. Cells are then expanded or exposed to different agents depending on the vaccine design. Active cells are then infused back to the patient. Cell concentration and viability are essential parameters to monitor throughout the manufacturing process. Ficoll is routinely used to isolate mononuclear cells from bone marrow, peripheral blood, and umbilical cord blood. Monocytes and lymphocytes form a buffy coat under a layer of plasma. Cells are collected and washed. Typically, some residual platelets or red blood cells are mixed in the mononuclear cells. The functional assays on cryopreserved PBMC are associated with viability of the cells. Viability thresholds should be used in clinical trials in order to obtain reliable results of functional assays.   Characteristics of PBMC experiments

• Cell quality degrades with time
• Large number of samples typically associated with clinical trials
• Cell sample are not pure, separation quality depends on patient sample and operator

Live PBMC concentration and viability are routinely measured for cell speration, processing and cryopreservation

• Label magnetic beads for cell separation
• Antibody labeling and staining for fluorescent active cell sorting and analysis
• Cryopreservation before adding cryo-cocktail
• Quality control after thawing

Challenges

Image-based cell counters rely on software to process cell images captured by a digital camera. Trypan blue stained cells appear fully dark in color while viable cells have a clear bright center. Software algorithms are able to identify the two populations to calculate cell concentration and % viability. Mononuclear cells mixed with platelets or red blood cells can interfere with the accurate identification of the live cells or trypan blue stained dead cells. Additional steps including lysing of the red blood cells are required to get accurate results with bright field counting of trypan blue stained cells. Optimization of cell size gating are also needed to avoid counting platelets.

Solution

Dual Fluorescence PBMC Concentration and Viability using Cellometer Auto 2000

The Acridine Orange/Propidium Iodide (AO/PI) viability assay is a rapid, highly linear, functuionally correlated assay that is superior to conventional viability measurement by trypan blue exlusion. (HPC viability measurement: trypan blue versus acridine orange and propidium iodide, K. Mascotti, J. McCullough, and S. R. Burger, Blood Components, Transfusion, Volume 40, June 2000)

•   *  Nuclear staining dyes to simply and accurately identify the live and dead mononuclear cells

  *  No need to lyse red blood cells

*  Not affected by platelets and other cell debris

]]> 3190 0 0 0 https://nebg.biobm.com/2012/08/12/nano-volume-drop-spectophotometry/ Sun, 12 Aug 2012 14:16:42 +0000 http://www.nebiogroup.com/?p=859 BioDrop is a new micro (nan0)-volume solution that overcomes many of the disadvantages of low volume instruments, providing the simplest way for life scientists to accurately quantify microlitre volumes of DNA and protein in almost any UV/Vis spectrophotometer.  Dr. Steve Church, a BioDrop scientist, discusses the advantages of this emerging technology:  ]]> 3191 0 0 0 https://nebg.biobm.com/2013/01/30/syngene-pxi-touch-gel-imaging-system/ Wed, 30 Jan 2013 01:24:06 +0000 http://www.nebiogroup.com/?p=930 Quick and simple touch screen set-up for perfect blots, 1D and 2D gel images Syngene, a world-leading manufacturer of gel documentation systems today announced the introduction of the new PXi Touch range of high resolution, multi-application image analysis systems. These easy-to-use systems are ideal for scientists wanting a rapid touch screen driven method of accurately imaging chemiluminescent and fluorescent blots, as well as 1-D and 2-D gels stained with any type of visible or fluorescent dye. The PXi Touch comes as a compact, ready to use unit with a built in touch screen and processor. Each PXi Touch features a camera with a large fixed aperture lens, which enables scientists to quickly capture images of even the faintest bands or spots, without having to spend time and effort learning how to adjust the camera’s settings. The PXi Touch is available in three versions with a high resolution 4, 6 or 8 million pixel camera for chemiluminescence and 1D and 2D gel applications. To enable researchers to choose the best and safest lighting for their fluorescent gel and blot applications, the PXi Touch has been cleverly designed for quick fit of a number of lighting and filter options to make the system versatile enough to use with Syngene’s UV and blue light transilluminators, as well as white, IR, red, blue or green epi lighting. The PXi Touch system’s touch screen is controlled by the intuitive GeneSys image acquisition and capture software, which means the PXi Touch can be rapidly set up to automatically select the best imaging conditions. With just one click scientists can generate perfect images of chemiluminescent blots, as well as 1-D and 2-D gels stained with any fluorescence, visible and IR commercial dyes. GeneTools, Syngene’s acclaimed image analysis software, also comes with the PXi Touch range and saves valuable time by automatically producing results in seconds, of applications including 1-D lane analysis, molecular weight and quantity calculations. Laura Sullivan, Syngene’s Divisional Manager stated: “Today’s scientists are familiar with touch screen icons in their everyday lives and would like to use these in the lab. Building on our successful PXi system we have responded to this need by adding a touch screen, and to make a complete, out of the box plug and play affordable system we have also integrated a powerful processor so that scientists do not need an external computer to control the PXi Touch.” Laura concluded: “The combination of high performance cameras across a range of applications, easy set up and affordability means the PXi Touch is the ideal multi-purpose workstation for laboratories where scientists demand versatility and rapid, high quality results from their chemi blots, 1-D and 2-D gel based imaging tasks.”]]> 3192 0 0 0 https://nebg.biobm.com/2013/01/30/blue-light-transilluminator-no-darkroom-requireddescre/ Wed, 30 Jan 2013 02:13:02 +0000 http://www.nebiogroup.com/?p=935 blue light transilluminator offers some unique advantages over UV transilluminators, especially when the intent is to use the system on top of the bench and out in the open.   Some of the unique advantages of the the blue light transilluminator over UB boxes is there is no risk of damaging or photobleaching DNA during excitation.  Ultraviolet excitation has been known  to  damage DNA via  thymine dimer formation and strand breaks.  This can lead to  decreased efficiency in cloning and transformation (Paabo et al. 1990, Grundmann and Schomig, 1996) A blue light transilluminator is also more safe to work with on the bench versus a UV transilluminator.   Exposure to ultraviolet radiation (commonly between 302nm and 315nm) during exposure on the bench can cause damage to human tissue in the eyes and skin.  "Ultraviolet ransilluminators have the potential effects on eyes (photokeratitis, photoconjunctivitis and  cataracts) and skin erythema (sunburn like condition) elastosis (photoaging and skin cancer).  Hands, arms, face and eyes are likely sites of injury. Working unprotected for even a few minutes can cause injury. Some individuals have greater than usual photosensitivity. " "Effects of UV can occur within a short period (photokeratitis and erythema) or a long period after  exposure (skin cancer). It is possible to calculate acute threshold for acute effects and therefore  set exposure limits, however it is not possible to do so for chronic effects, therefore there is no safe exposure level and exposure should be reduced as much as possible." Some recommended suggestions are as follows:

• Wear laboratory coats.
• Wear lab glove.
• Ensure skin is not exposed between the sleeves and gloves.
• Wear a face shield

Optimally, the UV transilluminator should be in a darkroom of a gel documentation system.  Syngene gel documentation systems offer complete UV safety and will automatically shut-off lights as the darkroom door is opened or after the image has been captured. Many researchers wish to cut bands from the gel and typically, this is performed on the bench.  As such, UV transilluminators are one on the most commonly found items in the lab.  However, a blue light transilluminator is a much safer instrument to work with on the bench.  A blue light transilluminator does not damage DNA and there is no risk of burning eyes or skin. With this in mind, a blue light transilluminator is recommended for use on the bench. New England BioGroup is committed to making sure researchers have access to blue light transilluminators at a reasonable price.  Safety should not be a financial decision!  We are available to speak further and help you select the blue light transilluminator that best fits your needs.  ]]> 3193 0 0 0 https://nebg.biobm.com/2013/10/05/blue-light-transilluminator-safety-information/ Sat, 05 Oct 2013 12:20:31 +0000 http://www.nebiogroup.com/?p=1020 http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2006:114:0038:0059:EN:PDF.  This document is very complex and calculating exposure time value depends on different energy, but CE also suggests the safest way is to not look directly into the light source for more than 10 seconds. So they treat 10 seconds as their suggested limitation.  We suggest as short as possible. According to a CELMA(Organization of manufacturing light source in Europe) report, they point out that looking at blue LED directly could cause the same damage as looking at sunshine directly, but human will close eyes automatically so it is very difficult to really cause damage. Based on the documents, Maestrogen believes, and this is in the user manual, looking at the blue LED directly will cause damage, as would looking at sunshine. But if the time is less than 10 second, it won’t cause any damage to human.  However looking directly into the blue light without the filter lid is never advised. SLB--01W and LB-16 provide the protective filter in front of the operator. For those who do not operate but just exposed under leaking blue light, it should not cause any damage.  In the user manual, Maestrogen also reminds users not to look at directly at the light source.  So, f they just follow the precautions in the user manual and exercise some common sense (10 secs or less of direct exposure and do not put your face close to the UltraBright), it should be very safe to use a SW-01W or LB-16.]]> 3194 0 0 0 https://nebg.biobm.com/2015/01/02/syngene-gel-documentation-used-by-major-university-for-high-resolution-dna-gel-and-western-blot-imaging/ Fri, 02 Jan 2015 16:20:58 +0000 http://www.nebiogroup.com/?p=1192 GBOX Chemi XRQ gel imaging system a winner at major European university Syngene, a world-leading manufacturer of sensitive image analysis solutions, is proud to announce its G:BOX Chemi XRQ is being used by scientists at a well-respected European University to accurately detect proteins in metabolic disorders including diabetes.

GBOX gel documentation system is used as a multi-task imager

Researchers at the major university are using a G:BOX Chemi XRQ multi-task imager to precisely analyse agarose gels of mouse and rabbit DNA stained with the safe fluorescent dye SybrSafe^®. They are also utilizing the G:BOX Chemi XRQ  to easily image both large and small SDS-PAGE gels and chemiluminescent Western blots of proteins. This is allowing scientists at the university to accurately detect proteins and is contributing to identifying potential therapeutic targets in chronic diseases such as diabetes.

Gel documentation system chemiluminescence that rivals film

A laboratory technician at the university states: “We often have to analyse large protein gels and blots with up to 26 lanes to detect proteins of 15-300 kDa and so have to leave our chemiluminescent Westerns developing for over 40 minutes with film to get good results. This means we need an imager which is versatile enough to cope with these very different demands.” The technician continues: “We reviewed three imaging systems from other suppliers for these tasks but none were as multi-talented as the G:BOX Chemi XRQ. We chose this imager because using GeneSys software, makes it easy to set up and we can leave the system unattended capturing chemi images, while we get on with other work. The system’s binning feature gives us incredible sensitivity, with the kind of results we’ve been getting using expensive X-ray films and we can fit even our largest gels and blots into the unit for analysis in one shot. For price and image quality this system is the clear winner in our lab.” “We’re excited to hear that in head to head comparisons the G:BOX Chemi XRQ comes out as the top performer at this well-respected European University,” comments Rob van den Broek, Syngene European Sales Manager. “Scientists looking for outstanding sensitivity and versatility from their imager should get in touch now to find out why owning a G:BOX Chemi XRQ system is a smart, risk-free choice.”

GBOX gel documentation price makes this a wonderful value for the lab

Any researchers in New England (MA, CT, RI, VT, ME, NH) can contact a New England BioGroup product specialist to learn more about these systems and to schedule a product demonstration. If you are located out of New England, please contact Syngene direct by visiting www.syngene.com.]]> 3195 0 0 0 https://nebg.biobm.com/2015/01/16/denovix-ds-11-versus-nanodrop-easy-apps-highlights/ Fri, 16 Jan 2015 12:55:54 +0000 http://www.nebiogroup.com/?p=1213 Download PDF of this page

 DeNovix DS-11 Spectrophotometer Key Apps Features

The DeNovix® DS-11 Spectrophotometer is a compact, stand-alone instrument with intuitive, easyto-use icon driven software. Pre-configured separate applications for each of the most commonly measured biomolecule types ensures that methods are optimized and simple to use.

Android OS on a stand-alone microvolume UV/Vis spectrophotometer

The Android operating system is today’s most widely used platform for mobile devices including smartphones and tablets. The direct swiping, tapping, pinching and reverse pinching manipulations provides an immediate and fluid user interface. The DeNovix DS-11 interface uses a customized Android OS which takes advantage of many of the touchscreen navigation features already familiar to many of todays’ researchers. In addition, the software uses standard Android icons such as the ones shown in the table below to eliminate the need for learning something unique to a new device.

 DeNovix DS-11 microvolume UV/Vis Spectrophotometer - Nucleic Acids

To streamline the busy lab’s workflow the three most common nucleic acid types (dsDNA, RNA and ssDNA) are measured launching distinct apps on the Home screen. This ensures each application is optimized for quick measurements. Sample type selections are automatic, saving time and reducing errors associated with using an incorrect factor for concentration calculations.

Proteins and Peptides

The DS-11 Protein A280 app includes an easy feature to save and recall new protein types. This convenience combined with the instrument’s ultra short pathlength capabilities enable measurements of protein samples with absorbance values of 500A (1 cm equivalent). No other spectrophotometer currently on the market can measure such highly concentrated proteins. The Peptide app may be used for low concentration peptides that do not contain aromatic amino acid residues. Peptides with aromatic residues should be measured using the Protein A280 app.

Labeled Nucleic Acids and Proteins

Microarray and Labeled Protein apps enable quick quality control measurements before running costly genomic and proteomic array experiments. The DS-11 software includes 11 pre-configured dyes and labels with the option to quickly add and save an unlimited number of additional dyes and fluorophores. This option ensures you will be able to take advantage of new dye technologies as they evolve.

Colorimetrics

The Colorimetric app is designed for easy routine BCA, Bradford, Lowry and Pierce 660 nm assays. Standard curves may be saved and recalled for ease of use. New colorimetric assays are easily added using the User Method app.

UV/Vis and Custom Methods

The DS-11 spectrophotometer can be used for more than nucleic acid and protein measurements! The simple UV-Vis app allows you to analyze samples with absorbance between 190 and 840 nm. The powerful Custom Std Curves and Custom Formula Methods apps have been designed as tools to create new standard curve and UV -Vis methods in the fewest possible steps. The custom formulas capability can save you the time associated with routine calculations.

Kinetics and OD600

The DS-11+ combined microvolume/cuvette model software includes a Kinetics app that lets you take advantage of a built in heater with a 37˚ to 45˚ C range for temperature controlled time vs absorbance measurements. The OD600 app is used to measure microbial cell culture suspensions.

Additional Apps

A search friendly Data app facilitates quick access to all sample data while password protected Accounts ensure the integrity of saved settings and methods. The Calculator app includes scientific oligo and dilution calculators in one convenient tool while the Updater app ensures you can stay up-to date with all the latest DS-11 software features.

Summary

The DS-11 software has been designed to take advantage of the full capabilities of the DS-11 spectrophotometer. The intuitive user interface is an excellent complement to the sleek, modern instrument design and ensures that routine measurements are quick and efficient. In summary, the DS-11 Spectrophotometer provides the ease-of-use and the powerful options necessary for today's research labs.

Interested in the DeNovix DS-11?

We are here to help! Please contact us to discuss. We can bring the instrument into the lab for a demonstration. We also offer free 1-week trial evaluations. Alternatively, you can visit us at an upcoming vendor show.  ]]> 3196 0 0 0 https://nebg.biobm.com/2015/01/17/stain-free-pre-cast-gel-visualization/ Sat, 17 Jan 2015 01:20:28 +0000 http://www.nebiogroup.com/?p=1232 Move over Bio-Rad! Syngene Gel Documentation Systems Can Be Used for Stain-Free Imaging.

Syngene G:BOX system helps save time and make protein analysis safer

Scientific Digital Imaging’s [SDI’s] Syngene Division, a world-leading manufacturer of image analysis solutions is delighted to announce that the lighting and filter conditions in the G:BOX image analysis system have been optimized to allow faster, safer visualization of proteins on stain-free gels.

Extensive testing for optimal imaging performance

Technical specialists at Syngene tested filter and lighting conditions in a G:BOX image analysis system to accurately image a range of proteins (2-300ng) run on a Bio-Rad Criterion TGX Stain-Free gel. For optimum imaging performance, the gel was exposed to 5 minutes of mid wave UV (302nm) on a UV transilluminator and the gel was imaged using a UV filter and an exposure time of 2 seconds. The gel was then stained with ProtoBlue Safe and imaged again. The G:BOX image analysis results from the stained and unstained gel showed the linearity and sensitivity of both methods of protein visualisation was comparable with proteins being detected in the 2-20ng range. In fact, with some proteins, the stain- free gels provided better detection limits than the ProtoBlue Safe dye staining.

Scientists can save time without compromising on sensitivity or safety

Dr. Lindsey Kirby, SDI’s Syngene Application Specialist explained: “Staining protein gels can be a time consuming task as scientists often use Coomassie Blue, which is toxic and requires a lengthy de-staining step. Therefore, if scientists can image their gels immediately after they have run them, this makes analysis safer and more efficient.” Lindsey added: “This is why we’ve worked to develop the optimal imaging conditions for stain-free visualisation. We’re delighted that our G:BOX technology is capable of such excellent performance in this application because biologists regularly working with protein gels can now confidently use the G:BOX image analysis system for rapid stain-free visualisation without compromising on sensitivity or safety.”

GBOX gel documentation price makes this a wonderful value for the lab

Any researchers in New England (MA, CT, RI, VT, ME, NH) can contact a New England BioGroup product specialist to learn more about these systems and to schedule a product demonstration. If you are located out of New England, please contact Syngene direct by visiting www.syngene.com.  ]]> 3197 0 0 0 https://nebg.biobm.com/2015/01/17/blotcycler-automated-western-blot-protocol-system/ Sat, 17 Jan 2015 16:07:35 +0000 http://www.nebiogroup.com/?p=1245 Precision Biosystems BlotCycler Enables Users to Automate Western Blot Protocols and Improve Data Quality Western blot is a widely used method to detect the presence of protein(s) of interest in a given sample and/or detect the post-translational modification such as phosphorylation. The proteins are run on the SDS-PAGE and then transferred onto the surface of the membrane, providing access to immunodetection reagents.  Immunodetection then proceeds as described in Fig.1. Fig. 1A Fig. 1B Fig. 1. Workflow of a typical immunodetection protocol by western blot. After nonspecific binding sites are blocked, usually by adding dry milk or Bovine Serum Albumin (BSA) to a wash buffer saline solution that contains a mild detergent, the membrane is probed with the primary antibody and then washed 3-5 times. The antibody-antigen complexes are then detected by a secondary antibody conjugated to a fluorophore (A) or an enzyme such as Horseradish Peroxidase (HRP) that converts a substrate into a light-emitting molecule (B). After 3-5 washes, the resulting complex is detected by fluorescence or chemiluminescence respectively.

Precision Biosystems BlotCycler Automates All Steps in a Western Blot Protocol Starting from Blocking

BlotCycler^TM automates all the steps in the protocol starting from blocking and up to the washing steps after the incubation with the secondary antibodies. Compared to the traditionally used manual protocols, BlotCycler^TM provides the end-user with an opportunity to program the precise duration of all the steps as well as the number of washes. Furthermore, the instrument’s shaking and fluidic systems ensure consistent and reproducible dispensing, mixing, and distribution of the reagents throughout the process and provide with an option to recycle primary antibodies. Finally, BlotCycler^TMenables a simultaneous processing of up to twelve blots, while using up to six different primary and secondary antibodies and two separate protocols. Taken together, BlotCycler^TM provides substantial savings in time, money, and increased throughput while enabling the standardization of the western blot procedure that is difficult to achieve using the manual processing.

Automated Western Blot Processing Provides Increased Sensitivity Over the Manual Procedure

It is a common practice to perform the incubation with primary antibody at 4⁰C overnight and conduct the rest of the steps at room temperature during the manual procedure, as a convenience of not having to frequently go to the 4⁰C room for solution changes. However this has been shown to eliminate advantages of incubation at low temperature on background and signal intensity. To further dissect the role of automation and incubation temperature in the increased signal, the manual and automatic processing were carried out entirely at 4°C or RT (Fig. 2). The results indicate that while both automation and 4°C alone increased the signal, the effect was most pronounced in combination of the two. Therefore, BlotCycler^TM offers the distinct advantage in improved sensitivity of western blotting procedure while eliminating the drudgery associated with the manual processing. Fig. 2A                                                                                                                                Fig. 2B Fig. 2. Automated western blot processing by BlotCycler^TM increased the signal as compared to the manual procedure. (A) Images of the blots processed as indicated. In each blot, the first lane contained a HeLa whole cell lysate, the second lane contained a recombinant Human IL-2, and the third lane a mixture of HeLa whole cell lysate and h-IL2. Following the SDS-PAGE electrophoresis, proteins were transferred from the gel onto the Nitrocellulose membrane. Membranes were blocked for 30 min at RT or for 90 min at 4ºC using MB-070. Rabbit anti-IL2 and mouse anti-α-tubulin primary antibodies were incubated for 90 min at RT or for 18 hours at 4°C. DyLight™ 549 conjugated anti-mouse IgG and DyLight™ 649 conjugated anti-rabbit IgG secondary antibodies were incubated for 30 min at RT or 90 min at 4°C. Data for each DyLight™ fluorophore were collected independently at excitation/emission wavelengths: 530nm/605nm for the DyLight™ 549 and 625nm/695nm for DyLight™ 649. The fluorescent intensity between different blots was normalized using MW standard as shown in (B).

Blotcycler System’s Western Blot processing is Highly Reproducible

To determine if the automated western blot processing results in highly reproducible signal, five identical blots were subjected to the analysis by BlotCycler^TM using the same antibodies as described in Fig. 2. The results on Fig. 3 demonstrated excellent reproducibility as seen visually (Fig. 3A). To confirm this quantitatively, the band intensity for each protein was quantified and normalized against the marker. The calculations shown in Fig. 3B are consistent with visual observations and suggest that the salient characteristics of BlotCycler^TM, such as timing and consistency of solution changes, efficient washing, and elimination of operator caused errors, would enable the much-needed standardization of western blotting   Fig. 3. Automatic western blot processing by BlotCycler^TM yielded highly reproducible results.  (A) Images of the five identical blots where the first lane contained a HeLa whole cell lysate, the second lane contained a recombinant Human IL-2, and the third lane had a mixture of HeLa whole cell lysate and h-IL2. After SDS-PAGE electrophoresis, proteins were transferred from the gel onto the Nitrocellulose membrane. Membranes were blocked for 90 min using MB-070. and then incubated with the rabbit anti-IL2 and mouse anti-α-tubulin primary antibodies for 18 hours, followed by the incubation with DyLight™ 549 conjugated anti-mouse IgG and DyLight™ 649 conjugated anti-rabbit IgG secondary antibodies for 90 min. All incubation steps were performed at 4⁰C. Data for each DyLight™ fluorophore was collected independently at excitation/emission wavelengths: 530/605 nm for the DyLight™ 549 and 625/695 nm for DyLight™ 649. The calculated fluorescent intensity between different blots was normalized using MW standard as shown in (B).

Throughput and Reproducibility of BlotCycler Enabled Rigorous Optimization of the Antibody Concentration

Optimal concentrations of both primary and secondary antibodies against a given antigen are essential to obtain the most informative results and are highly dependent on their specific activities. BlotCycler^TM allows simultaneous processing of up to six different blots with six different secondary or primary antibodies at the same time with high reproducibility, thereby avoiding the inherent slight variations associated with the manual processing of multiple blots at the same time. An example of such optimization is shown in Fig. 4. Here, ten identical strips that contained MW marker and mouse pancreas extract were prepared and probed with five different dilutions of primary antibody against Delta-4 (Fig. 4A) and a single dilution of secondary antibody. The second experiment contained a single dilution of primary antibody) and six different dilutions of the secondary antibody (Fig. 4B). Using BlotCycler™ we were able to construct the titration curves for both primary and secondary antibodies. Fig. 4A Fig. 4B Fig. 4. BlotCycler^TM enabled optimization of antibody concentrations. (A) Titration of the primary rabbit anti-Delta-4 antibody with a fixed secondary DyLight™ 649 conjugated anti-rabbit IgG antibody concentration (1:20,000). (B) Titration of the secondary antibody with a fixed primary antibody concentration (1:1,000). The gel was loaded with an extract of mouse pancreas in all lanes. Electrophoresis was run as described. Proteins were transferred from the gel onto the Nitrocellulose membrane. Membranes were blocked for 90 min using MB-070, and then incubated with the primary antibody for 18 hours, followed by the incubation with the secondary antibody for 90 min. All incubation steps were performed at 4°C. Data was collected independently using band pass filter at 625/695 nm for DyLight™ 649. The fluorescent intensity between different blots was normalized using MW standard.

BlotCycler price makes this a wonderful value for the lab

Any researchers in New England (MA, CT, RI, VT, ME, NH) can contact a New England BioGroup product specialist to learn more about these systems and to schedule a product demonstration. If you are located out of New England, please contact Precision Biosystems direct by visiting www.precisionbiosystems.com.]]> 3198 0 0 0 https://nebg.biobm.com/2015/05/01/syngene-launches-sensitive-pxi-access-imaging-range-for-unrivalled-detection-of-faint-bands-on-large-blots-and-gels/ Fri, 01 May 2015 19:21:40 +0000 http://www.nebiogroup.com/?p=1278 Cambridge, UK: Syngene, a world-leading manufacturer of image analysis solutions today introduced the new PXi Access range of compact, multi-application imaging systems. The easy-to-use PXi Access systems offer major advances on film detection and can rapidly generate high quality images of virtually all types of large blots and gels. The new compact PXi 6 or 9 Access systems are complete with high performance cameras capable of imaging a wide range of blot and gel types and sizes. The camera in each PXi Access features superb optics, which means scientists can set up quickly and detect even the faintest amounts of DNA or protein with a much greater sensitivity than film. PXi Access is so flexible that the systems can image stain-free gels and blots or those stained with any commercial chemiluminescence, fluorescence, visible and IR dyes.  For imaging chemi blots, the PXi Access uses a wide dynamic range to optimise exposure for each protein, accurately quantifying abundant and poorly expressed proteins, making this a much simpler method than using film for producing Western blot results. Featuring a touch screen controlled by GeneSys software, PXi Access Touch offers an intuitive user experience that makes capturing images so simple. In just one click scientists can have publication-quality images of blots and gels (up to 21cm x 17cm) with a PXi 6 and 9 Access. Scientists wanting to find out more about the sensitive PXi Access range, can click this link for details:http://www.syngene.co.uk/pxi-pxi-touch/ “We are delighted to be introducing affordable imagers that perform so amazingly across a range of diverse applications,” says Martin Biggs, Syngene’s UK & Ireland Sales Manager, “the combination of technology and expertise in the PXi Access range, makes them this year’s go to systems for laboratories demanding unrivalled versatility and high quality results from their western blot and gel analysis.”]]> 3199 0 0 0 https://nebg.biobm.com/?post_type=product&p=1690 Fri, 23 Feb 2024 11:03:52 +0000 https://nebg.biobm.com/?post_type=product&p=1690 1690 0 62 0 https://nebg.biobm.com/?post_type=product&p=1691 Fri, 23 Feb 2024 11:04:34 +0000 https://nebg.biobm.com/?post_type=product&p=1691 1691 0 63 0 https://nebg.biobm.com/?post_type=product&p=1692 Fri, 23 Feb 2024 11:05:26 +0000 https://nebg.biobm.com/?post_type=product&p=1692 1692 0 64 0 https://nebg.biobm.com/?post_type=product&p=1694 Fri, 23 Feb 2024 11:06:27 +0000 https://nebg.biobm.com/?post_type=product&p=1694 1694 0 65 0 https://nebg.biobm.com/?post_type=product&p=1695 Fri, 23 Feb 2024 11:06:59 +0000 https://nebg.biobm.com/?post_type=product&p=1695 1695 0 66 0 https://nebg.biobm.com/?post_type=product&p=1696 Fri, 23 Feb 2024 11:08:01 +0000 https://nebg.biobm.com/?post_type=product&p=1696 1696 0 67 0 https://nebg.biobm.com/?post_type=product&p=1698 Fri, 23 Feb 2024 11:09:14 +0000 https://nebg.biobm.com/?post_type=product&p=1698 Gel trays are not included.]]> Gel trays are not included.]]> 1698 0 68 0 https://nebg.biobm.com/?post_type=product&p=1700 Fri, 23 Feb 2024 11:11:39 +0000 https://nebg.biobm.com/?post_type=product&p=1700 Gel trays are not included.]]> 1700 0 69 0 https://nebg.biobm.com/?post_type=product&p=1701 Fri, 23 Feb 2024 11:12:34 +0000 https://nebg.biobm.com/?post_type=product&p=1701 1701 0 70 0 https://nebg.biobm.com/?post_type=product&p=1702 Fri, 23 Feb 2024 11:13:52 +0000 https://nebg.biobm.com/?post_type=product&p=1702 1702 0 71 0 https://nebg.biobm.com/?post_type=product&p=1705 Fri, 23 Feb 2024 11:16:13 +0000 https://nebg.biobm.com/?post_type=product&p=1705 1705 0 72 0 https://nebg.biobm.com/?post_type=product&p=1706 Fri, 23 Feb 2024 11:19:42 +0000 https://nebg.biobm.com/?post_type=product&p=1706 Galileo Reflection™ 1010 Dual-Gel Vertical Electrophoresis Unit by Galileo bioscience Run either one or two gels simultaneously on these versatile separation systems. The 10x10cm size of the system also accomodates most pre-cast gels. Complete double sided vertical system for running one or two gels, in a 10 x 10 cm format. Unique casting bases allows both casting and running of gels without unclamping from the upper buffer chamber, providing for safer and more convenient gel handling. In addition the 1010 device will accommodate most precast gel cassettes on the market. Water cooling feature is included for protocols that require it. Connections are easily made by attaching standard tubing over the 3/8" barbed in/out ports provided on the device. Water may be provided by tap or other temperature controlled water source. Optional alumina backer plates may be used to facilitate cooling efficiency and uniformity, and are available in the accessories list below.

System Features

The GalileoReflection™ 85-1010 complete system includes these features:

• Heavy duty upper buffer chamber with cooling port connections.
• Lower buffer chamber.
• Interlock safety lid with attached leads.
• Casting base.
• Two-pack each of plain & notched glass plates.
• One acrylic blocker plate (used when running only one gel).
• Ttwo 10-well, 0.8mm thick combs
• Four 0.8mm thick side spacers.

System accessories

Choose amoung these accessories to customize each system for your specific needs.

• Alumina cooling plate. For use with cooling systems. This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
• Casting base. For quick, efficient and leak-proof casting.
• Multiple gradient caster.

View the Galileo Bioscience electrophoresis and blotting system catalog]]> System accessories Choose amoung these accessories to customize each system for your specific needs.

• Alumina cooling plate. For use with cooling systems. This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
• Casting base. For quick, efficient and leak-proof casting.
• Multiple gradient caster.

]]> 1706 0 73 0 https://nebg.biobm.com/?post_type=product&p=1708 Fri, 23 Feb 2024 11:20:23 +0000 https://nebg.biobm.com/?post_type=product&p=1708 Galileo Reflection™ 1614 Dual-Gel large-Format Vertical Electrophoresis Unit by Galileo bioscience Run either one or two large format gels simultaneously on these versatile separation systems.  The 1614 unit is the ideal unit for separation of complex analytes. Designed to run one or two larger format 16cm wide x 14cm tall gels, the unit provides longer run lengths and increased separation capacity. The unique casting base allows both casting and running of the gel without unclamping from the upper buffer chamber, providing for safer and more convenient gel handling. Water cooling feature is included for protocols that require it. Connections are easily made by attaching standard tubing over the 3/8" barbed in/out ports provided on the device. Water may be provided by tap or other temperature controlled water source. Optional alumina backer plates may be used to facilitate cooling efficiency and uniformity, and is shown in the accessories list below.

System Features

The GalileoReflection™ 85-1614 complete system includes these features:

• Heavy duty upper buffer chamber with cooling port connections.
• Lower buffer chamber.
• Interlock safety lid with attached leads.
• Casting base.
• Four-pack each of plain & notched glass plates
• One acrylic blocker plate (used when running only one gel)
• Two 10-well, 1.5mm thick combs.
• Two 15-well, 1.5mm thick combs.
• Eight 1.5mm thick side spacers

System accessories

Choose amoung these accessories to customize each system for your specific needs.

• Alumina cooling plate. For use with cooling systems. This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
• Casting base. For quick, efficient and leak-proof casting.

View the Galileo Bioscience electrophoresis and blotting system catalog]]> System accessories Choose amoung these accessories to customize each system for your specific needs.

• Alumina cooling plate. For use with cooling systems. This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
• Casting base. For quick, efficient and leak-proof casting.

]]> 1708 0 74 0 https://nebg.biobm.com/?post_type=product&p=1710 Fri, 23 Feb 2024 11:29:00 +0000 https://nebg.biobm.com/?post_type=product&p=1710 1710 0 60 0 https://nebg.biobm.com/?post_type=product&p=1711 Fri, 23 Feb 2024 11:29:23 +0000 https://nebg.biobm.com/?post_type=product&p=1711 1711 0 36 0 https://nebg.biobm.com/?post_type=product&p=1712 Fri, 23 Feb 2024 11:30:52 +0000 https://nebg.biobm.com/?post_type=product&p=1712 1712 0 37 0 https://nebg.biobm.com/?post_type=product&p=1713 Fri, 23 Feb 2024 11:31:45 +0000 https://nebg.biobm.com/?post_type=product&p=1713 1713 0 38 0 https://nebg.biobm.com/?post_type=product&p=1714 Fri, 23 Feb 2024 11:33:44 +0000 https://nebg.biobm.com/?post_type=product&p=1714 1714 0 39 0 https://nebg.biobm.com/?post_type=product&p=1715 Fri, 23 Feb 2024 11:34:27 +0000 https://nebg.biobm.com/?post_type=product&p=1715 1715 0 40 0 https://nebg.biobm.com/?post_type=product&p=1716 Fri, 23 Feb 2024 11:36:49 +0000 https://nebg.biobm.com/?post_type=product&p=1716 1716 0 41 0 https://nebg.biobm.com/?post_type=product&p=1717 Fri, 23 Feb 2024 11:43:19 +0000 https://nebg.biobm.com/?post_type=product&p=1717 1717 0 42 0 https://nebg.biobm.com/?post_type=product&p=1718 Fri, 23 Feb 2024 11:44:24 +0000 https://nebg.biobm.com/?post_type=product&p=1718 1718 0 43 0 https://nebg.biobm.com/?post_type=product&p=1719 Fri, 23 Feb 2024 11:46:17 +0000 https://nebg.biobm.com/?post_type=product&p=1719 1719 0 45 0 https://nebg.biobm.com/?post_type=product&p=1720 Fri, 23 Feb 2024 11:47:30 +0000 https://nebg.biobm.com/?post_type=product&p=1720 1720 0 46 0 https://nebg.biobm.com/?post_type=product&p=1721 Fri, 23 Feb 2024 11:48:04 +0000 https://nebg.biobm.com/?post_type=product&p=1721 1721 0 49 0 https://nebg.biobm.com/?post_type=product&p=1722 Fri, 23 Feb 2024 11:48:57 +0000 https://nebg.biobm.com/?post_type=product&p=1722 1722 0 50 0 https://nebg.biobm.com/?post_type=product&p=1723 Fri, 23 Feb 2024 11:49:37 +0000 https://nebg.biobm.com/?post_type=product&p=1723 1723 0 51 0 https://nebg.biobm.com/?post_type=product&p=1724 Fri, 23 Feb 2024 11:50:05 +0000 https://nebg.biobm.com/?post_type=product&p=1724 1724 0 57 0 https://nebg.biobm.com/?post_type=product&p=1725 Fri, 23 Feb 2024 11:50:49 +0000 https://nebg.biobm.com/?post_type=product&p=1725 1725 0 54 0 https://nebg.biobm.com/?post_type=product&p=1726 Fri, 23 Feb 2024 11:51:18 +0000 https://nebg.biobm.com/?post_type=product&p=1726 1726 0 56 0 https://nebg.biobm.com/?post_type=product&p=1727 Fri, 23 Feb 2024 11:51:59 +0000 https://nebg.biobm.com/?post_type=product&p=1727 1727 0 53 0 https://nebg.biobm.com/?post_type=product&p=1728 Fri, 23 Feb 2024 11:52:51 +0000 https://nebg.biobm.com/?post_type=product&p=1728 1728 0 55 0 https://nebg.biobm.com/?post_type=product&p=1729 Fri, 23 Feb 2024 11:53:36 +0000 https://nebg.biobm.com/?post_type=product&p=1729 1729 0 52 0 https://nebg.biobm.com/?post_type=product&p=1730 Fri, 23 Feb 2024 11:54:12 +0000 https://nebg.biobm.com/?post_type=product&p=1730 1730 0 48 0 https://nebg.biobm.com/?post_type=product&p=1731 Fri, 23 Feb 2024 11:55:35 +0000 https://nebg.biobm.com/?post_type=product&p=1731 Gel trays are not included.]]> Gel trays are not included.]]> 1731 0 58 0 https://nebg.biobm.com/?post_type=product&p=1732 Fri, 23 Feb 2024 11:56:45 +0000 https://nebg.biobm.com/?post_type=product&p=1732 1732 0 59 0 https://nebg.biobm.com/?post_type=product&p=1733 Fri, 23 Feb 2024 11:57:48 +0000 https://nebg.biobm.com/?post_type=product&p=1733 1515 Gel Unit by Galileo bioscience Extra width for increased sample thru-put. Holds up to 112 samples.Unit features RapidCast trays for quick in-unit gel casting of 12cm wide by 14cm long gels.

System description

The 1515 is ideal for guick screening of multiple samples, and if you choose Galileo microtiter combs, quick multi-channel loading too! The unit features the Galileo ExpressCast gel casting system for quick, tape-free gel casting and includes a built-in bubble level and leveling screws for convenient level adjustment. You may choose from a wide variety of comb options to customize your order.

System Features

The Galileo 1515 complete system includes these features:

• Heavy duty lower buffer chamber
• Interlock safety lid with attached leads
• End gated 15cm wide x 15cm long ExpressCast™ UV-transparent gel tray with built-in fluorescent ruler and 14 comb slots.
• Two 1.5mm x 17 tooth combs
• Two 1.5mm x 34 tooth combs

System accessories

Choose amoung these accessories to customize each system for your specific needs.

• Additional gel trays.
• A wide range of comb options.
• Gel loading guides. These guides fit into the comb slots of the gel tray, and have precision machined slots that provide accurate aligning of your multi-channel pipet during gel loading.

View the Galileo Bioscience electrophoresis and blotting system catalog]]> System description The 1515 is ideal for guick screening of multiple samples, and if you choose Galileo microtiter combs, quick multi-channel loading too! The unit features the Galileo ExpressCast gel casting system for quick, tape-free gel casting and includes a built-in bubble level and leveling screws for convenient level adjustment. You may choose from a wide variety of comb options to customize your order.]]> 1733 0 47 0 https://nebg.biobm.com/?post_type=product&p=1735 Fri, 23 Feb 2024 12:00:05 +0000 https://nebg.biobm.com/?post_type=product&p=1735 1214 Mini Gel Unit by Galileo bioscience This flexible system from Galileo is the perfect personal unit for a wide range of separation needs, and our most popular model. Unit features RapidCast trays for quick in-unit gel casting of 12cm wide by 14cm long gels.

System description

Choose a complete system or build your own custom unit from the many system components we offer.  The G-80-1214 gel system is supplied with a RapidCast™ gasketed gel tray for convenient gel casting directly in the buffer chamber.  Choose from a wide variety of comb options.  All are compatible with Owl Separation Systems B2A or similar devices.  The teeth on combs marked as "microtiter" are uniformly spaced for easy loading with multichannel pipettors.System features

System Features

The 80-1214 complete system includes these features:

• Heavy duty lower buffer chamber
• Interlock safety lid with attached leads
• Gasketed (cast in place) 12cm wide x 14cm long UV-transparent gel tray with
• built-in fluorescent ruler and 2 comb slots.
• One 1.5mm x 12 tooth comb
• One 1.5mm x 20 tooth comb

System Options

The Galileo 1214 unit can be also be ordered with our new automatic buffer recirculation system (80-1214-R) or with installed buffer ports for connection to an external buffer recirculation system (80-1214-BEP)

System accessories

Choose amoung these accessories to customize each system for your specific needs.

• RapidCast casting base G-80-1214-RCGC. Accommodates up to three 1214 RapidCast gel trays for casting outside buffer chamber.
• Gel loading guide G-80-1214-MT2SLG. This guide fits into the comb slots of the gel tray, where it's precision machined slots provide accurate positioning for your multi-channel pipet during gel loading.

View the Galileo Bioscience electrophoresis and blotting system catalog]]> System description Choose a complete system or build your own custom unit from the many system components we offer.  The G-80-1214 gel system is supplied with a RapidCast™ gasketed gel tray for convenient gel casting directly in the buffer chamber.  Choose from a wide variety of comb options.  All are compatible with Owl Separation Systems B2A or similar devices.  The teeth on combs marked as "microtiter" are uniformly spaced for easy loading with multichannel pipettors.System features]]> 1735 0 44 0 https://nebg.biobm.com/?post_type=product&p=1738 Fri, 23 Feb 2024 12:03:46 +0000 https://nebg.biobm.com/?post_type=product&p=1738 1325 Gel Unit by Galileo bioscience The Galileo 1325 unit is a medium capacity system with long run lengths for high resolution. The ExpressCast™ gel casting system makes gel pouring quick, easy and tape-less! The 1325 is a medium capacity system. With a maximum run length of 25cm, the unit can provide high resolution of complex analytes. Or, fitted with maximum capacity combs in all of the tray's 6 comb slots, the unit can handle up to 112 samples. The 2325 features the Galileo ExpressCast gel casting system for quick, tape-free gel casting and includes a built-in bubble level and leveling screws for convenient level adjustment. You may choose from a wide variety of comb options to customize your order.

System Features

The Galileo 1325 complete system includes these features:

• Heavy duty lower buffer chamber
• Interlock safety lid with attached leads
• End gated 13cm wide x 25cm long ExpressCast™ UV-transparent gel tray with built-in fluorescent ruler and 6 comb slots.
• One 1.5mm x 12 tooth combs
• One 1.5mm x 16 tooth combs
• One 1.5mm x 20 tooth combs

System accessories

Choose amoung these accessories to customize each system for your specific needs.

• Additional gel trays.
• A wide range of comb options.
• Gel loading guides. These guides fit into the comb slots of the gel tray, and have precision machined slots that provide accurate aligning of your multi-channel pipet during gel loading.

View the Galileo Bioscience electrophoresis and blotting system catalog]]> ]]> 1738 0 15 0 https://nebg.biobm.com/?post_type=product&p=1740 Fri, 23 Feb 2024 12:06:27 +0000 https://nebg.biobm.com/?post_type=product&p=1740 1525 Gel Unit by Galileo bioscience Perfectly sized for multi-channel sample loading. Holds up to XXX samples.System description The 1525 is a long run length mini-gel system. With a maximum run length of 25cm, the unit can provide high resolution of complex analytes. Or, fitted for maximum capacity, the unit can handle up to xxx samples. The 1525 features the Galileo ExpressCast gel casting system for quick, tape-free gel casting and includes a built-in bubble level and leveling screws for convenient level adjustment. You may choose from a wide variety of comb options to customize your order.

System Features

The Galileo 1525 complete system includes these features:

• Heavy duty lower buffer chamber
• Interlock safety lid with attached leads
• End gated 15cm wide x 25cm long ExpressCast™ UV-transparent gel tray with built-in fluorescent ruler and 14 comb slots.
• Two 1.5mm x 17 tooth combs
• Two 1.5mm x 34 tooth combs

System accessories

Choose amoung these accessories to customize each system for your specific needs.

• Additional gel trays.
• A wide range of comb options.
• Gel loading guides. These guides fit into the comb slots of the gel tray, and have precision machined slots that provide accurate aligning of your multi-channel pipet during gel loading.

View the Galileo Bioscience electrophoresis and blotting system catalog]]> Perfectly sized for multi-channel sample loading. Holds up to XXX samples.System description The 1525 is a long run length mini-gel system. With a maximum run length of 25cm, the unit can provide high resolution of complex analytes. Or, fitted for maximum capacity, the unit can handle up to xxx samples. The 1525 features the Galileo ExpressCast gel casting system for quick, tape-free gel casting and includes a built-in bubble level and leveling screws for convenient level adjustment. You may choose from a wide variety of comb options to customize your order.]]> 1740 0 16 0 https://nebg.biobm.com/product/zoom-ht-lb-920-plate-washer-microplate-washer/ Tue, 20 Feb 2024 09:34:38 +0000 https://nebg.biobm.com/?post_type=product&p=222 MAXIMUM SPEED AND RELIABILITY The Zoom HT microplate washer is a robust and easy-to-use system that delivers the speed you need for high throughput plate washing. The unmatched speed of the system is the result of the integrated stackers and a one-rail design. This plate washer can process both 96 and 384 well microplates without changing the wash head. The fastest plate washer on the market today It takes only 17 seconds for a triple wash of a 96 well microplate. Combined with the unique one-rail plate transfer design which moves the plate a very short distance from the stack to the wash position. This enables a typical throughput of 150 washed plates per hour, or up to 1050 plates per day (3 x 300 µL, 7 h per day, 1 single Zoom HT).   A single wash head for all plate types Each wash channel has its own aspirate and dispense tip, which allows simultaneous washing of all 96 wells. The same 96 channel wash head is used for 384 well washing in four quadrants, making it unnecessary to purchase different wash heads for different plate types. Rapid positioning of the wash head provides 384 well wash speeds comparable to 384 well channel washers. Large diameter aspirate tips, 3-dimensional (3D) tip positioning and a constant flow rate vacuum pump ensure a smooth and efficient washing experience. Individual configuration of the washing protocol Two wash modes are available: Regular wash mode and Superwash mode (also known as “Overflow mode”). When using the regular wash mode, the wash fluid that is dispensing and evacuated follow each other. When using the Superwash mode, the wash fluid that is dispensing and evacuated are conducted simultaneously creating a continuous wash fluid flow throughout the entire well. The Zoom HT supports creating and storing of the 3D tip positioning profile for each plate type and assay. This profile defines the aspirate tip horizontal and vertical positions thereby mapping how the well is aspirated. In addition, the speed with which the tip is immersed into the well is adjustable. Fine-tuning of all the parameters of the wash procedure will allow to wash even loosely adherent cultured cells. The Zoom HT will have residual wash fluid volumes of less than 1 μL/well, this can be achieved when a surfactant is present in the wash fluid. A unique self-emptying liquid discharge system The discarded wash fluids are captured by a self-emptying waste trap, which allows uninterrupted processing of large plate batches. Biohazard waste management is supported by the automatic addition of a decontamination solution which is continuously drained from the trap.     Automatic switch of buffer liquids The Zoom HT is available in 3 different configurations using a one, two or four wash buffer inlet. This is advantageous for applications that require sequential cleaning with different buffers or for an automatic cleaning routine after using the system.images Easy maintenance The unique design of the dispense manifold eliminates the need for any special cleaning. The aspirate head is maintenance free because of the large aspirate tip diameter. This is combined with a continuous vacuum that reliably prevents any accumulation of reagent residue. Optional Dispense Module The optional Dispense Module is an easy-to-install plug-and-play device, is extremely easy to use and requires no calibration. With the addition of a dispense module the Zoom HT Washer becomes an efficient washer-dispenser combination system. The Dispensing Module provides single or dual-channel dispensing of reagents using direct drive and positive displacement syringes to ensure the highest precision and accuracy when dispensing. The dispense module feeds one or two 8- or 16-way manifolds for 96 and/or 384 well microplates       TECHNICAL DETAILS (ZOOM HT WASHER) Wash head: 96 channel wash head for fast processing of 96 and 384 well microplates Wash modes: Standard and Superwash Wash fluid selection: 1, 2 or 4 inlet versions Wash volume: 5-300 µL Aspirate tip descent speeds: Slow, Medium, Fast Soak time: 0-99 s Shaking: Up to 80 s, amplitude 1 mm, frequency 12 Hz Number of programs: Up to 99 Wash cycles per program: Up to 99]]> 222 0 96 0 https://nebg.biobm.com/product/x-clarity-tissue-clearing-system/ Wed, 21 Feb 2024 08:57:32 +0000 https://nebg.biobm.com/?post_type=product&p=588 X-CLARITY II Tissue Clearing System SECTIONING IS TIME. WE CUT THE TIME Tissues are inherently three dimensional in nature, which makes imaging intact tissues a necessity for a more complete study in the relationship between structure and function and the system-level study of cellular mechanisms. Tissue clearing has become an important step for imaging tissues in 3D at single-cell resolution. The X-CLARITY™ is a collection of systems and ready-to-use reagents to standardize, simplify, and accelerate each step of the tissue clearing process. X-CLARITY™ is based on CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging / Immunostaining /in situ-hybridization-compatible Tissue hYdrogel), a tissue clearing method developed by the Deisseroth lab at Stanford. With CLARITY, preserved tissues are embedded in a hydrogel matrix and lipids are actively extracted through electrophoresis to create a stable and optically transparent tissue-hydrogel hybrid that is chemically accessible for multiple rounds of antibody labeling and imaging. Native cytoarchitecture remains intact and even endogenous fluorescence proteins are preserved for robust fluorescence imaging downstream. Features Easy installation, Ultrafast clearing, Reliable results The new X-CLARITY II Tissue Clearing System from the imaging specialists at Logos Biosystems provides an all-in-one solution for electrophoretic tissue clearing. The groundbreaking CLARITY™ method developed by the Deisseroth Lab at Stanford University (Chung et al, Nature 497:332-337) provides unparalleled tissue samples for imaging. Combining an innovative electrode design and an integrated cooling system, the X-CLARITY™ Tissue Clearing System allows for faster and more consistent tissue clearing. Many researchers currently attempting the CLARITY™ method often encounter difficulties that range from instrument construction to poor clarifying, resulting in the loss of valuable samples. Days or even weeks spent processing a precious sample can end in frustration and disappointment. The X-CLARITY™ Tissue Clearing System greatly simplifies the CLARITY™ process, can clarify a whole adult mouse brain in under 48 hours, and dramatically increases your chances of obtaining transparent tissue samples for use in downstream labeling.   HOW IT WORKS ? Typical negative effects related with conventional electrophoretic tissue clearing methods include burning, melting, the formation of black precipitates, and bubble trapping. The main causes of these artifacts are due to irregular electric currents and poor temperature control during electrophoresis. The X-CLARITY™ dramatically reduces the negative effects associated with standard electrophoretic tissue clearing with a unique electrode design and an active cooling system. The X-CLARITY™ Tissue Clearing System is composed of four components: 1) the X-CLARITY™ ETC Controller, 2) X-CLARITY™ ETC Chamber 3) X-CLARITY™ pump and 4) X-CLARITY™ buffer reservoir. The X-CLARITY™ ETC Controller works as both a power supply and temperature controller. At the heart of the X-CLARITY™ Tissue Clearing System is the X-CLARITY™ ETC Chamber. A tissue sample is placed inside the X-CLARITY™ ETC Chamber where it undergoes electrophoresis to remove lipids. Clearing buffer is continually circulated through the chamber under temperature controlled conditions, minimizing the probability of artifacts arising due to poor temperature control. Highlights Exceptional transparency Fastest processing time Easy installation and operation Minimized artifacts No leaking, no boiling Pre-optimized protocol ACCELERATE YOUR RESEARCH WITH THE X-CLARITY™ TISSUE CLEARING SYSTEM Compared to other methods, the X-CLARITY™ Tissue Clearing System dramatically reduces tissue clearing time. A whole adult mouse brain can be cleared in under 48 hours. Depending on the thickness, mouse brain slices may be cleared much faster. Additionally, the speed of the X-CLARITY™ Tissue Clearing System makes optimizing for other organs and tissues a much easier endeavor. One of the most exciting features of the CLARITY™ technology is that, it allows for the penetration of and labeling with macromolecules such as antibodies or oligonucleotides, generating unprecedented 3D images that show the relationship between structure and function. Chemical tissue clearing technologies introduced in recent years generally do not accommodate labeling with macromolecules. CLARITY™ overcomes this limitation and even enables multiple rounds of antibody labeling. The X-CLARITY™ Tissue Clearing System also successfully produces samples that demonstrate the efficient penetration of antibodies. WHAT IS CLARITY™ ? CLARITY™ is a novel technology that produces a transparent tissues by forming a hydrogel network that supports the ultrastructure while allowing for the removal of lipids. Unlike other tissue clearing methods, the CLARITY™ uniquely enables the efficient labeling of tissues with macromolecules such as antibodies and nucleic acids, making high resolution imaging possible. CLARITY™ is expected to overcome the current limitation of light microscopy technology, which does not allow the imaging of tissues without first creating slices. CLARITY™ is the trademark of Stanford University. ]]> 588 0 95 0 https://nebg.biobm.com/product/tristar-3-multimode-reader-microplate-reader/ Thu, 22 Feb 2024 12:54:51 +0000 https://nebg.biobm.com/?post_type=product&p=1652 SIMPLICITY AND SENSITIVITY FOR ALL LEVELS OF EXPERIENCE The Tristar 3 is a user-friendly and affordable filter-based multimode plate reader that offers the high-performance analysis you expect from Berthold Technologies instruments. Equipped with ONE-4-ALL optics for uncompromised performance of all detection modes, the system is ready to perform absorbance, luminescence and fluorescence measurements. It provides you with the flexibility for today, tomorrow, and beyond to master your changing plate reading applications:

• High-sensitivity Luminescence
• BRET
• Absorbance (UV/VIS)
• Fluorescence
• FRET

Broad wavelength range selection from UV through the visible range

From UV absorbance for DNA quantification to measurements in the far red or NIR, the Tristar 3 is ready for any assay and greatly improves the wavelength range of the TriStar².

BRET/BRET2 and NanoBRET™ upgradable

All Tristar 3 preconfigured models have the features you need to perform BRET assays: best-in-class luminescence sensitivity and a high quality filters. To start working with BRET, just order the correct filters for BRET1, BRET2, and NanoBRET™.

ONE-4-ALL Optics for uncompromised performance

Berthold Technollogies' patented ONE-4-ALL optics have been optimized to combine the stability and user-friendliness of a multimodal optical system with the sensitivity and versatility of a dedicated optical devices. ONE-4-ALL optics work with both filter and monochromator based applications, so there is never a compromise in performance in any mode.

High-performance filter system - when sensitivity counts

The quick-change filter technology of the Tristar series provides you with the flexibility required to meet your application needs. With your choice of up to 40 different excitation and emission filters can be easily mounted on exchangeable filter slides. The filters are characterized by high transmittance properties which can be up to 25-fold more sensitive than monochromators. Technologies like Time-Resolved Fluorescence is measured more efficiently with filters. Furthermore, filters with a large bandwidth are available to analyze fluorophores with wide spectra and all luminescence-based assays requiring the use of filters, e.g. BRET, BRET2 and NanoBRET™ assays.

Ergonomic design to support your work

The ergonomic design of the system provides full frontal access for all key operations (e.g. plate loading, filter change and connecting reagents). Reagent vials can be stored in the integrated front compartment providing easy access and visibility. The Tristar 3 contains a removable trough that can be filled with water or ice to keep all of your temperature sensitive reagents cooled. Different reagent tube holders are available to ensure secure handling of valuable reagents. The system has a flat surface on top, providing enough space for a laptop.

Powerful crosstalk control – reliable results

Do you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples?  Then you are potentially affected by a phenomenon called crosstalk, this can produce wrong results and wrong conclusions. The crosstalk reduction design of the Tristar 3 almost eliminates this problem: with a crosstalk of 10^-6, you will never have to worry about the position of your high-intensity samples in your plate.

JET injectors: highest accuracy, speed and cell-friendliness

The Tristar 3 can be equipped with up to 3 JET injectors with variable volumes in pre-measurement or measurement position. The ultra-fast injection speed of JET injectors eliminates any delay between injection and measurement. This enables the Tristar 3 to measure ultra fast reactions, e.g. flash luminescence. Berthold Technologies JET injectors use Teflon bellows for accurate and fast injections. This guarantees the most efficient mixing as well as extreme longevity.

• Highest accuracy and reproducibility with greater than 98 % accuracy and reproducibility over the entire volume range.
• Low reagent consumption due to the low dead volume and the injectors can recover up to 60 % of the reagent in the injector line. A single priming sequence is enough to achieve a 99 % homogeneous mix at the tip.
• Low maintenance costs – friction-free operation enables more than 3 million injections without mechanical failure.
• Optimal performance in flash-kinetics - ultra-fast injection enables the measurement of the first 150 ms of a kinetic assay (e.g. Fura-2 or Acridinium ester assays).
• Worry-free injection of cell suspensions - negligible shear forces are ideal when working with living cells, e.g. in Aequorin-based calcium assays.

Convenient Software options

Our microplate readers are powered by LightCompass®, a flexible software enabling easy data acquisition, analysis and reporting using Berthold Technologies' instruments. It has been optimized for different microplate and tube formats. LightCompass® features all the functions you can expect from an advanced scientific software, such as flexible creation of measurement protocols, curve fitting, qualitative analysis and compliance with FDA 21 CFR Part 11. LightCompass® is available in 3 different versions: Basic, Plus and Professional. Just pick the version that better suits your research needs.

Validation tools, Service & Support

For many laboratories ensuring compliance with a number of GMP and GLP requirements is essential. We provide a number of tools and services to help you ensure that your system is running at peak performance.

Validation Tools

We provide a variety of tools to help you check and confirm the performance of your products over time.

Service

Berthold Technologies provides a team of dedicated factory-trained service engineers and experts to optimize your productivity. We and our local partners are always at your disposal.

Support

Our team of technical support scientists is your resource to overcome the unique challenges your application brings. Contact our team to discuss your assay, experiment design, data analysis, troubleshooting questions.]]>

High-sensitivity Luminescence

BRET

Absorbance (UV/VIS)

Fluorescence

FRET

]]> 1652 0 87 0 https://nebg.biobm.com/product/syngene-gbox-chemi-xrq-gel-documentation-and-western-blot-imaging/ Fri, 23 Feb 2024 08:47:21 +0000 https://nebg.biobm.com/?post_type=product&p=1671 Syngene is the most popular gel documentation system in New England.

If raw data, high sensitivity, image resolution, an affordable price, and great service are important factors in making your decision for a gel imaging system, contact us or call (617)286-4632.

Cost-efficient chemi imaging and gel doc

For a laboratory that needs hassle-free chemiluminescent detection, as well as routine gel documentation, using the G:BOX Chemi XRQ’s powerful GeneSys software to switch between applications is simplicity itself. Place your chemi blot in, and the system’s cooled camera captures images with a sensitivity equal to film or slide in your gels to generate quality pictures of your DNA and proteins anytime you want. Featuring a standard darkroom and a 4 million pixel image resolution, the G:BOX Chemi XRQ camera is cooled to provide a low signal to noise ratio with perfect background. HI-LED lighting options  cover the full spectrum of high intensity blue, green, red and infra-red resulting in faster exposure times and publication quality images. The G:BOX Chemi XRQ is a cost-effective alternative to laser based technology offering a faster workflow for imaging complex multiplex fluorescent gels and blots. The system is controlled by GeneSys application driven image capture software and comes complete with unlimited copies of GeneTools analysis software.

Features

Gel imaging for fluorescence and chemiluminescence

• High quantum efficiency (QE) camera Picogram detection
• Multiplex imaging of up to 5 different channels with UV, blue and RGB HI-LED lighting options Easily switch between blot or gel imaging
• Stain-free imaging capability Capture images of TGX Stain-Free™ FastCast™ acrylamide gels and many more
• Auto-exposure Perfectly exposed chemi images without film
• Excellent signal to noise ratio No annoying backgrounds on your blots
• Protocol driven image capture No camera expertise needed for great results
• Motor driven lens and filter wheel Simple, automated set up
• Reproducible and quantifiable data Accurate results time after time
• CFR21 Part 11 compliant Fully traceable results suitable for regulatory audits
• GeneSys application driven image capture software Contains extensive database of dyes and imaging protocols. All you need to know is the type of gel you’re using and GeneSys automatically selects the optimal lighting and filters to produce the perfect image
• GeneTools analysis software (unlimited copies) Analyse data at your own computer

Specifications

G:BOX Chemi XRQ
Image resolution (pixels m)	4
Effective resolution (pixels m)	16
A/D	16 bit
Greyscales	65536
Quantum efficiency @ 425nm	73%
Cooling	Peltier
Lens (motor driven)	Motor driven zoom 1.2
Filter wheel (7 position motor driven)	Yes
UV filter	Yes
Use with external PC	Yes
Darkroom
Standard	Yes
Illumination
Epi LED white lights	Yes
Epi UV 302nm	Optional
Epi red LED module	Optional
Epi blue LED module	Optional
Epi green LED module	Optional
Epi red LED module M series for multiplexing	Optional
Epi green LED module M series for multiplexing	Optional
Epi blue LED module M series for multiplexing	Optional
Epi IR LED module	Optional
IR multiplexing kit (680-800nm)	Optional
HI-LED RGB	Optional
HI-LED RGBIR	Optional
HI-LED RIR	Optional
Visible light converter 33 x 31cm	Optional
White light pad for visible stains (20 x 14cm)	Optional
UltraBright LED blue light transilluminator 20 x 16cm	Optional
UV transilluminators	Optional
Dimensions
Max image area (cm)	30.5 x 22.7
Min image area (cm)	5.1 x 3.8
W x H x D (cm)	57 x 84 x 45
Weight (kg)	37
Voltage	115v/240v

]]> Cost-efficient chemi imaging and gel doc For a laboratory that needs hassle-free chemiluminescent detection, as well as routine gel documentation, using the G:BOX Chemi XRQ’s powerful GeneSys software to switch between applications is simplicity itself. Place your chemi blot in, and the system’s cooled camera captures images with a sensitivity equal to film or slide in your gels to generate quality pictures of your DNA and proteins anytime you want.]]> 1671 0 77 0 https://nebg.biobm.com/product/syngene-gbox-chemi-xx6-gel-documentation-and-ecl-detection/ Fri, 23 Feb 2024 08:47:44 +0000 https://nebg.biobm.com/?post_type=product&p=1673 Syngene is the most popular gel documentation system in New England.

If raw data, high sensitivity, image resolution, an affordable price, and great service are important factors in making your decision for a gel  imaging system, contact us or call (617)286-4632.

G:BOX CHEMI XX6

For high performance imaging, the G:BOX Chemi series systems provide the user with an extended range of applications covering fluorescence and chemiluminescence.

Walk Away Western Blot Imaging

Your lab’s imaging system shouldn’t control how you detect proteins on Western blots. Chemiluminescence is great if you want sensitive detection of picogram or femtogram amounts, while fluorescence lets you quantify and detect multiple different proteins on one blot. With a G:BOX Chemi XX6/XX9 you can have it all. Powered by Syngene’s revolutionary GeneSys software featuring hundreds of imaging protocols, you’re free to choose chemiluminescence and fluorescence reagents from any manufacturer, putting you in charge of how you detect your proteins. Featuring an extended darkroom, you can choose between a 6 or 9 mega pixel, cooled, high quantum efficiency camera for unrivaled levels of sensitivity with minimal background interference. HI-LED lighting options  cover the full spectrum of high intensity blue, green, red and infra-red resulting in faster exposure times and publication quality images. An edge lighting option can also be used for 2D gel capture including the use of DIGE gels. The system is controlled by GeneSys application driven image capture software and comes complete with unlimited copies of GeneTools analysis software.

Features

Gel imaging for fluorescence and chemiluminescence

• High quantum efficiency (QE) camera For unrivalled levels of sensitivity
• Fast chemiluminescence detection Walk-away image capture without film
• Motor driven lens and filter wheel Easy to use, fully automated set up
• IR and RGB HI-LED lighting options Multiplex fluorescence and protein quantification
• Stain-free imaging capability Capture images of TGX Stain-Free™ FastCast™ acrylamide gels and many more
• Edge lighting option 2D and multiplex in-gel fluorescence DIGE image capture
• White, UV and blue lighting options Capture all types of gel and blot images with one system
• Extended darkroom with motor driven stage Easily image large gels and blots
• GeneSys application driven image capture software Contains extensive database of dyes and imaging protocols. All you need to know is the type of gel you’re using and GeneSys automatically selects the optimal lighting and filters to produce the perfect image
• GeneTools analysis software (unlimited copies) Analyse data at your own computer

Specifications

Specifications

	G:BOX Chemi XX6	G:BOX Chemi XX9
Image resolution (pixels m)	6	9.1
Effective resolution (pixels m)	18	27
A/D	16 bit	16 bit
Greyscales	65536	65536
Quantum efficiency @ 425nm	73%	73%
Cooling	Peltier	Peltier
Lens (motor driven)	Motor driven f0.95 with auto focus and stage zoom	Motor driven f0.95 with auto focus and stage zoom
Filter wheel (7 position motor driven)	Yes	Yes
UV filter	Yes	Yes
Use with external PC	Yes	Yes
Darkroom
Extended with motor driven stage	Yes	Yes
Illumination
Epi LED white lights	Yes	Yes
Epi UV 302nm	Optional	Optional
Epi red LED module	Optional	Optional
Epi blue LED module	Optional	Optional
Epi green LED module	Optional	Optional
Epi red LED module M series for multiplexing	Optional	Optional
Epi green LED module M series for multiplexing	Optional	Optional
Epi blue LED module M series for multiplexing	Optional	Optional
Epi IR LED module	Optional	Optional
IR multiplexing kit (680-800nm)	Optional	Optional
HI-LED RGB	Optional	Optional
HI-LED RGBIR	Optional	Optional
HI-LED RIR	Optional	Optional
Visible light converter 33 x 31cm	Optional	Optional
White light pad for visible stains (20 x 14cm)	Optional	Optional
UltraBright LED blue light transilluminator 20 x 16cm	Optional	Optional
Edge lighting unit 26.5 x 20cm	Optional	Optional
UV transilluminators	Optional	Optional
Dimensions
Max image area (cm)	32.3 x 25.6	32.3 x 25.6
Min image area (cm)	15 x 11.8	15 x 11.8
W x H x D (cm)	57 x 99 x 55	57 x 99 x 55
Weight (kg)	45	45
Voltage	115v/240v	115v/240v

]]> G:BOX CHEMI XX6 For high performance imaging, the G:BOX Chemi series systems provide the user with an extended range of applications covering fluorescence and chemiluminescence.]]> 1673 0 78 0 https://nebg.biobm.com/product/syngene-gbox-chemi-xx9-gel-documentation-and-western-blot-imaging/ Fri, 23 Feb 2024 08:52:17 +0000 https://nebg.biobm.com/?post_type=product&p=1675 Syngene is the most popular gel documentation system in New England

If raw data, high sensitivity, image resolution, an affordable price, and great service are important factors in making your decision for a gel  imaging system, contact us or call (617)286-4632.

Walk Away Western Blot Imaging

Your lab’s imaging system shouldn’t control how you detect proteins on Western blots. Chemiluminescence is great if you want sensitive detection of picogram or femtogram amounts, while fluorescence lets you quantify and detect multiple different proteins on one blot. With a G:BOX Chemi XX6/XX9 you can have it all. Powered by Syngene’s revolutionary GeneSys software featuring hundreds of imaging protocols, you’re free to choose chemiluminescence and fluorescence reagents from any manufacturer, putting you in charge of how you detect your proteins. Featuring an extended darkroom, you can choose between a 6 or 9 mega pixel, cooled, high quantum efficiency camera for unrivaled levels of sensitivity with minimal background interference. HI-LED lighting options  cover the full spectrum of high intensity blue, green, red and infra-red resulting in faster exposure times and publication quality images. An edge lighting option can also be used for 2D gel capture including the use of DIGE gels. The system is controlled by GeneSys application driven image capture software and comes complete with unlimited copies of GeneTools analysis software.

Features

Gel imaging for fluorescence and chemiluminescence

• High quantum efficiency (QE) camera For unrivalled levels of sensitivity
• Fast chemiluminescence detection Walk-away image capture without film
• Motor driven lens and filter wheel Easy to use, fully automated set up
• IR and RGB HI-LED lighting options Multiplex fluorescence and protein quantification
• Stain-free imaging capability Capture images of TGX Stain-Free™ FastCast™ acrylamide gels and many more
• Edge lighting option 2D and multiplex in-gel fluorescence DIGE image capture
• White, UV and blue lighting options Capture all types of gel and blot images with one system
• Extended darkroom with motor driven stage Easily image large gels and blots
• GeneSys application driven image capture software Contains extensive database of dyes and imaging protocols. All you need to know is the type of gel you’re using and GeneSys automatically selects the optimal lighting and filters to produce the perfect image
• GeneTools analysis software (unlimited copies) Analyse data at your own computer

Specifications

	G:BOX Chemi XX6	G:BOX Chemi XX9
Image resolution (pixels m)	6	9.1
Effective resolution (pixels m)	18	27
A/D	16 bit	16 bit
Greyscales	65536	65536
Quantum efficiency @ 425nm	73%	73%
Cooling	Peltier	Peltier
Lens (motor driven)	Motor driven f0.95 with auto focus and stage zoom	Motor driven f0.95 with auto focus and stage zoom
Filter wheel (7 position motor driven)	Yes	Yes
UV filter	Yes	Yes
Use with external PC	Yes	Yes
Darkroom
Extended with motor driven stage	Yes	Yes
Illumination
Epi LED white lights	Yes	Yes
Epi UV 302nm	Optional	Optional
Epi red LED module	Optional	Optional
Epi blue LED module	Optional	Optional
Epi green LED module	Optional	Optional
Epi red LED module M series for multiplexing	Optional	Optional
Epi green LED module M series for multiplexing	Optional	Optional
Epi blue LED module M series for multiplexing	Optional	Optional
Epi IR LED module	Optional	Optional
IR multiplexing kit (680-800nm)	Optional	Optional
HI-LED RGB	Optional	Optional
HI-LED RGBIR	Optional	Optional
HI-LED RIR	Optional	Optional
Visible light converter 33 x 31cm	Optional	Optional
White light pad for visible stains (20 x 14cm)	Optional	Optional
UltraBright LED blue light transilluminator 20 x 16cm	Optional	Optional
Edge lighting unit 26.5 x 20cm	Optional	Optional
UV transilluminators	Optional	Optional
Dimensions
Max image area (cm)	32.3 x 25.6	32.3 x 25.6
Min image area (cm)	15 x 11.8	15 x 11.8
W x H x D (cm)	57 x 99 x 55	57 x 99 x 55
Weight (kg)	45	45
Voltage	115v/240v	115v/240v

]]> Walk Away Western Blot Imaging Your lab’s imaging system shouldn’t control how you detect proteins on Western blots. Chemiluminescence is great if you want sensitive detection of picogram or femtogram amounts, while fluorescence lets you quantify and detect multiple different proteins on one blot. With a G:BOX Chemi XX6/XX9 you can have it all. Powered by Syngene’s revolutionary GeneSys software featuring hundreds of imaging protocols, you’re free to choose chemiluminescence and fluorescence reagents from any manufacturer, putting you in charge of how you detect your proteins.]]> 1675 0 79 0 https://nebg.biobm.com/product/syngene-gbox-f3-gel-documentation-system/ Fri, 23 Feb 2024 09:37:17 +0000 https://nebg.biobm.com/?post_type=product&p=1676 Syngene is the most popular gel documentation system in New England.

If raw data, high sensitivity, image resolution, an affordable price, and great service are important factors in making your decision for a gel  imaging system, contact us or call (617)286-4632.

Versatile high resolution gel doc

An entry level system for imaging fluorescence and visible applications, the G:BOX F3 gel doc is the ideal solution for your laboratory. Using your choice of dyes or stains, the G:BOX F3 automatically selects the right lighting and filters to detect close bands on both small and large gels. Choosing white light options for Coomassie Blue, UV and blue lighting options, ethidium bromide or SYBR^® Safe gels, this system is the ultimate in gel doc flexibility. The stylish and modular designed G:BOX F3 gel doc system includes a high resolution 5m pixel camera which is capable of giving outstanding images with incredible spatial resolution as well as a motor driven zoom lens and a motor driven filter wheel as standard. G:BOX F3 also comes with a Lens Feedback option allowing you to optimise your image capture using user protocols. The system is controlled by GeneSys application driven image capture software and comes complete with unlimited copies of GeneTools analysis software.

Features

Gel imaging for fluorescence and visible applications

• Spacious darkroom Image gels up to 32cm x 24cm
• 5m pixel camera Resolves close bands and detects nanogram amounts
• Motor driven zoom lens and filter wheel One click set up for easy image capture
• Optional Lens Feedback available Optimise image capture by using user protocols
• White UV and blue lighting options Flexibility to image fluorescent and visible stained gels
• GeneSys application driven image capture software Contains extensive database of dyes and imaging protocols. All you need to know is the type of gel you’re using and GeneSys automatically selects the optimal lighting and filters to produce the perfect image
• GeneTools analysis software (unlimited copies) Analyse data at your own computer

Specifications

G:BOX F3
Image resolution (pixels m)	5
Effective resolution (pixels m)	15.1
A/D	12/16 bit
Greyscales	65536
Quantum efficiency @ 425nm	52%
Cooling	None
Lens (motor driven)	Zoom f1.2. Optional f1.2 with lens feedback
Filter wheel (7 position motor driven)	Yes
UV filter	Yes
Use with external PC	Yes
Darkroom
Standard	Yes
Illumination
Epi LED white lights	Yes
Epi UV 302nm	Optional
Visible light converter 33 x 31cm	Optional
White light pad for visible stains (20 x 14cm)	Optional
UltraBright LED blue light transilluminator 20 x 16cm	Optional
UV transilluminators	Optional
Dimensions
Max image area (cm)	32.5 x 24.1
Min image area (cm)	5.6 x 4.2
W x H x D (cm)	57 x 84 x 45
Weight (kg)	37
Voltage	115v/240v

]]> Versatile high resolution gel doc An entry level system for imaging fluorescence and visible applications, the G:BOX F3 gel doc is the ideal solution for your laboratory.]]> 1676 0 80 0 https://nebg.biobm.com/product/syngene-gbox-mini-6-or-9-gel-documentation-and-western-blot-imaging/ Fri, 23 Feb 2024 09:44:30 +0000 https://nebg.biobm.com/?post_type=product&p=1678 Syngene is the most popular gel documentation system in New England

If raw data, high sensitivity, image resolution, an affordable price, and great service are important factors in making your decision for a gel  imaging system, contact us or call (617)286-4632.

Compact fluorescent and chemi Western blot imaging system

Need to quantify multiple different fluorescent proteins on one blot and capture images of chemi blots, but your lab is limited on space? Then the G:BOX mini is just the compact imaging powerhouse you’ve been looking for. With stain-free, RGB and IR excitation options for Alexa Fluor^® 488, 546, 647 and IR dyes, you’re just a click away from brilliant multiplex images from one single Western. Using the G:BOX mini you’ll also get perfectly exposed images of your chemi labelled proteins without the bother of film. Simply place your blot in the darkroom, click on GeneSys capture software and your imager will do the rest with no fuss and no messy chemicals. G:BOX mini is a compact, multi-application imaging system combining a unique motor driven stage and cooled high resolution 6 or 9 megapixel camera enabling you to generate accurate optical images, not just digitally enhanced ones. With a G:BOX mini you’ll see separate close chemi and fluorescent bands or spots even on complex gels and know they’re really part of your data. HI-LED lighting options  cover the full spectrum of high intensity blue, green, red and infra-red resulting in faster exposure times and publication quality images. The system is controlled by GeneSys application driven image capture software and comes complete with unlimited copies of GeneTools analysis software.

Feature

Compact fluorescent and chemi Western blot imaging system

• Small footprint Leaves plenty of room on any lab bench
• RGB and IR HI-LED lighting options Fast quantification of multiple fluorescent proteins without stripping and re-probing
• Stain-free imaging capability Capture images of TGX Stain-Free™ FastCast™ acrylamide gels and many more
• High quantum efficiency (QE) camera Detect picogram or femtogram amounts
• Luxury lens (F/0.95 motor driven) with data feedback Captures the highest quality images
• Automatic motor driven stage with automated focus For real, optical images
• White, UV and blue lighting options Image all gels and blot types on one system
• Cleverly designed screen mount option Easily and securely attach a monitor
• GeneSys application driven image capture software Contains extensive database of dyes and imaging protocols. All you need to know is the type of gel you’re using and GeneSys automatically selects the optimal lighting and filters to produce the perfect image
• GeneTools analysis software (unlimited copies) Analyse data at your own computer

Specifications

Detection with a difference

SYSTEM	G:BOX mini 6	G:BOX mini 9
Image resolution (megapixels)	6	9.1
Effective resolution (megapixels)	18	27
A/D	16 bit	16 bit
Greyscale	65,536	65,536
Quantum efficiency (@425nm)	73%	73%
Lens (motor driven)	F0.95	F0.95
Motor driven stage	True optical imaging	True optical imaging
Filter wheel (7 position motor driven)	Yes	Yes
UV filter	Yes	Yes
Use with external PC and printer (not included)	Yes	Yes
LIGHTING
Epi LED white lights	Yes	Yes
HI-LED (red, blue, green)	Optional	Optional
HI-LED (red, infrared)	Optional	Optional
HI-LED (red, blue, green, infrared)	Optional	Optional
Visible light converter	Optional	Optional
Blue converter screen	Optional	Optional
Slim slide-out UV transilluminator (20cm x 20cm)	Optional – 254, 302, 365nm wavelengths	Optional – 254, 302, 365nm wavelengths
UltraSlim Blue LED transilluminator Gel size (cm)	Optional10 x 12	Optional 10 x 12
DIMENSIONS
Max image area (cm)	15 x 12	15 x 12
Min image area (cm)	10 x 8	10 x 8
W x H x D (cm)	40 x 64 x 52	40 x 64 x 52
Weight (kg)	40	40
Power input (V)	100-240	100-240

]]> Compact fluorescent and chemi Western blot imaging system Need to quantify multiple different fluorescent proteins on one blot and capture images of chemi blots, but your lab is limited on space? Then the G:BOX mini is just the compact imaging powerhouse you’ve been looking for. With stain-free, RGB and IR excitation options for Alexa Fluor^® 488, 546, 647 and IR dyes, you’re just a click away from brilliant multiplex images from one single Western.]]> 1678 0 81 0 https://nebg.biobm.com/product/syngene-genegnome-xrq-western-blot-imager/ Fri, 23 Feb 2024 09:49:48 +0000 https://nebg.biobm.com/?post_type=product&p=1681 Syngene is the most popular gel documentation system in New England.

If raw data, high sensitivity, image resolution, an affordable price, and great service are important factors in making your decision for a gel imaging system, contact us or call (617)286-4632.

A high sensitivity chemiluminescent detection system for your Western blot. User defined protocol, no focusing, a three year warranty and great price. Base model under $10K

Dedicated chemiluminescence imaging system – great Westerns without film!

You love the sensitivity that chemi brings to Western blot imaging but hate the drama of using film to get it. Utilising the GeneGnome XRQ’s optimised short ‘camera to sample’ technology, you’ll get more than double the dynamic range of film, making it easy for you to see picogram or femtogram protein levels without all the fuss. The GeneGnomeXRQ is dedicated to chemiluminescence imaging and is built for high performance and automation, featuring a high quantum efficiency CCD camera. The compact instrument sits on any laboratory bench taking up very little space. The GeneGnome XRQ requires an external PC for use. The system is controlled by GeneSys application driven image capture software and comes complete with unlimited copies of GeneTools analysis software.

Features

Dedicated chemi imaging system

• Small footprint More space for your research
• High quantum efficiency (QE) camera Detects your faintest bands
• Automated chemiluminescent capture Perfect exposure without film
• Cooled camera No annoying background noise on your blots
• Fixed lens controlled by GeneSys software One click and you’ll image any manufacturer’s chemi reagents
• GeneSys application driven image capture software Contains extensive database of dyes and imaging protocols. All you need to know is the type of blot you’re using and GeneSys automatically selects the optimal lighting and filters to produce the perfect image
• GeneTools analysis software (unlimited copies) Analyse data on your own computer
• Optimised – for imaging chemiluminescence Western blots GeneGnome XRQ is configured for maximum sensitivity to ensure even the faintest band on a blot can be captured.
• Sensitive cooled camera The high quantum efficiency cooled CCD camera is very sensitive to low level light emissions from a blot. The peltier cooled camera has exceptional ‘signal to noise’ performance resulting in virtually undetectable background noise. The short ‘camera to sample’ distance further enhances the ability to work with chemiluminescence samples and reagents. When compared to film, the GeneGnome XRQ has more than double the dynamic range. This allows for extremely accurate quantification.
• Small footprint The GeneGnome XRQ has a very small footprint and takes up minimal bench space. The automatic slide-out drawer allows easy sample handling. The fixed aperture and focus of the lens results in a very simple set-up procedure making image capture as simple as a click of a mouse button.

Specifications

GeneGnome XRQ
Image resolution (pixels m)	4
Effective resolution (pixels m)	16
A/D	16 bit
Greyscales	65536
Quantum efficiency @ 425nm	73%
Cooling	Peltier
Lens (motor driven)	Fixed f0.95
Sample size (cm)	11 x 8
Requires external PC	Yes
Epi LED white lights	Yes
W x H x D (cm)	37.5 x 44 x 43

]]> Dedicated chemiluminescence imaging system – great Westerns without film! You love the sensitivity that chemi brings to Western blot imaging but hate the drama of using film to get it. Utilising the GeneGnome XRQ’s optimised short ‘camera to sample’ technology, you’ll get more than double the dynamic range of film, making it easy for you to see picogram or femtogram protein levels without all the fuss.]]> 1681 0 82 0 https://nebg.biobm.com/product/syngene-ingenius3-gel-documentation-system-2/ Fri, 23 Feb 2024 09:54:15 +0000 https://nebg.biobm.com/?post_type=product&p=1683 Download brochure

Economical gel documentation – DNA and protein gel imaging your way

Using a compact, InGenuis3 gel documentation system you’re in control. You can image your choice of dyes and stains by choosing UV, blue or white lighting options. The system’s GeneSys software selects the right lighting and filters for you, making it easy to image fluorescent ethidium bromide or SYBR^® Safe DNA gels and protein gels stained with Coomassie Blue or silver. For an economical system, the InGenius3 offers a 3m pixel camera and can produce images with 16 bit image depth. A manual zoom lens and manual filter drawer (includes a UV filter) completes the package. The system is controlled by GeneSys application driven image capture software and comes complete with unlimited copies of GeneTools analysis software.

Features

DNA and protein gel imaging your way

• Small footprint Plenty of bench space for your experiments
• 3m pixel camera Detect nanogram amounts on gels (up to 20cm x 20cm)
• Manual zoom Puts you in control of the camera
• White, UV and blue lighting options Flexibility to image fluorescent DNA and visibly stained protein gels
• High resolution InGenius3 uses a high resolution 3m pixel camera with a 16 bit image output
• Manual system Allows the user to select filters using a filter drawer, control lighting and the camera lens
• GeneSys application driven image capture software Contains extensive database of dyes and imaging protocols. All you need to know is the type of gel you’re using and GeneSys automatically selects the optimal lighting and filters to produce the perfect image
• GeneTools analysis software (unlimited copies) Analyse data at your own computer.

Product specifications

Camera	InGenius3
Sensor	1/3 inch
Resolution	3 million pixels
Image depth	12/16 bit
Greyscales	4,096/65,536
Dynamic range	3.6 – 4.8
Lens	Manual zoom 6.5 – 39, F1.4
Maximum viewing area	20 x 20cm
Illumination
Slim UV transilluminator 20 x 20cm – various single wavelengths are available	Option
UltraSlim-LED blue light transilluminator 10 x 12cm	Option
White Epi overhead	Yes
Converters
UV/visible light NovaGlo converter	Option
Software
GeneSys image capture	Yes
GeneTools image analysis	Yes
GeneDirectory	Option
Conversion screen
Novaglo	UV to visible
Size
Dimensions (h x d x w) cm	62 x 37.5 x 40

]]> ® Safe DNA gels and protein gels stained with Coomassie Blue or silver.]]> 1683 0 84 0 https://nebg.biobm.com/?post_type=product&p=1742 Fri, 23 Feb 2024 12:20:36 +0000 https://nebg.biobm.com/?post_type=product&p=1742 2314 Gel Unit by Galileo bioscience A high-capacity system in a mini-gel size, the Galileo 2314 unit enables rapid separation of large numbers of samples. The 2314 wide format system accomodates up to 200 samples per run, for quick separation and high thru-put. Because of the wide format of the Galileo 2314 unit, the 23cm wide by 14cm long tray is non-gasketed. Gel casting can be accomplished by taping, or we recommend the G-80-WADJ-CST multi-casting unit for even quicker and more convenient gel casting. The gel tray is UV transmissible, includes an imprinted fluorescent ruler, and has 4 comb slots. Choose from a wide variety of comb options, all with micro-titer spacing for easy loading with multi-channel pipets.

System Features

The Galileo 2314 complete system includes these features:

• Heavy duty lower buffer chamber
• Interlock safety lid with attached leads
• Non-gasketed 23cm wide x 14cm long UV-transparent gel tray with built-in fluorescent ruler and 4 comb slots.
• Four 1.5mm x 50 tooth combs
• Opaque well visualization plate.

System accessories

Choose amoung these accessories to customize each system for your specific needs.

• G-80-WADJ-CST multi casting platform.
• Additional 4 slot gel trays.
• A wide range of comb options.
• Gel loading guides. These guides fit into the comb slots of the gel tray, and have precision machined slots that provide accurate aligning of your multi-channel pipettor during gel loading.

View the Galileo Bioscience electrophoresis and blotting system catalog]]> Perfectly sized for multi-channel sample loading. Holds up to XXX samples.System description The 1525 is a long run length mini-gel system. With a maximum run length of 25cm, the unit can provide high resolution of complex analytes. Or, fitted for maximum capacity, the unit can handle up to xxx samples. The 1525 features the Galileo ExpressCast gel casting system for quick, tape-free gel casting and includes a built-in bubble level and leveling screws for convenient level adjustment. You may choose from a wide variety of comb options to customize your order.]]> 1742 0 17 0 https://nebg.biobm.com/?post_type=product&p=1744 Fri, 23 Feb 2024 12:22:40 +0000 https://nebg.biobm.com/?post_type=product&p=1744 2325 Gel Unit by Galileo bioscience Need long run lengths for maximum resolution, and large sample capacity too? Look at the features of the Galileo 2325 unit and we think you will find just what you need. The 2325 is a full sized large capacity system. With a maximum run length of 25cm, the unit can provide high resolution of complex analytes. Or, fitted for maximum capacity, the unit can handle up to 500 samples. The 2325 features the Galileo ExpressCast gel casting system for quick, tape-free gel casting and includes a built-in bubble level and leveling screws for convenient level adjustment. You may choose from a wide variety of comb options to customize your order.

System Features

The Galileo 2325 complete system includes these features:

• Heavy duty lower buffer chamber
• Interlock safety lid with attached leads
• End gated 23cm wide x 25cm long ExpressCast™ UV-transparent gel tray with built-in fluorescent ruler and 14 comb slots.
• Two 1.5mm x 25 tooth combs
• Two 1.5mm x 50 tooth combs

System accessories

Choose amoung these accessories to customize each system for your specific needs.

• Additional gel trays.
• A wide range of comb options.
• Gel loading guides. These guides fit into the comb slots of the gel tray, and have precision machined slots that provide accurate aligning of your multi-channel pipet during gel loading.

View the Galileo Bioscience electrophoresis and blotting system catalog]]> 1744 0 18 0 https://nebg.biobm.com/?post_type=product&p=1746 Fri, 23 Feb 2024 12:24:14 +0000 https://nebg.biobm.com/?post_type=product&p=1746 2340 Gel Unit by Galileo bioscience For long run lengths, maximum resolution, and really large sample capacity, look no further than the Galileo 2340 device. This unit also features the Galileo ExpressCast system for quick tape-less gel pouring.System description The 2340 is our maximum capacity system. With a maximum run length of 25cm, the unit can provide very high resolution of complex analytes. Or, fitted with maximum capacity combs in all of the tray's 12 comb slots, the unit can handle up to 600 samples. The 2340 features the Galileo ExpressCast gel casting system for quick, tape-free gel casting and includes a built-in bubble level and leveling screws for convenient level adjustment. You may choose from a wide variety of comb options to customize your order.

System Features

The Galileo 2340 complete system includes these features:

• Heavy duty lower buffer chamber
• Interlock safety lid with attached leads
• End gated 23cm wide x 40cm long ExpressCast™ UV-transparent gel tray with built-in fluorescent ruler and 12 comb slots.
• Two 1.5mm x 25 tooth combs
• Two 1.5mm x 50 tooth combs

System accessories

Choose amoung these accessories to customize each system for your specific needs.

• Additional gel trays.
• A wide range of comb options.
• Gel loading guides. These guides fit into the comb slots of the gel tray, and have precision machined slots that provide accurate aligning of your multi-channel pipet during gel loading.

View the Galileo Bioscience electrophoresis and blotting system catalog]]> 1746 0 19 0 https://nebg.biobm.com/?post_type=product&p=1748 Fri, 23 Feb 2024 12:28:53 +0000 https://nebg.biobm.com/?post_type=product&p=1748 Insight Mini Tank Blotter by Galileo bioscience A tank style blotting sytems for reliable and efficient molecular transfer of even "hard to transfer" compounds. This tank style electroblotter provides reliable and efficient transfer of a wide range of protein coumpounds from acrylamide gels. Up to four gels can be transfered simultaneously. Platinum grid style electrodes and robust construction assure even and complete molecular transfer and long service life. System is supplied complete with power leads and four blotting cassetes. Also included is a sample pack of our ultra-pure cotton fiber filter paper. View the Galileo Bioscience electrophoresis and blotting system catalog]]> 1748 0 23 0 https://nebg.biobm.com/?post_type=product&p=1749 Fri, 23 Feb 2024 12:30:05 +0000 https://nebg.biobm.com/?post_type=product&p=1749 0708 Mini Gel Unit by Galileo bioscience Perfectly sized for rapid analysis of small analytes like PCR and RE fragments. This unit features a RapidCast gasketed tray for quick in-unit gel casting.

System description

The standard 0708 mini gel system includes a RapiCast™ gasketed gel tray for convenient gel casting right in the buffer chamber.

System features

The 0708 complete system includes these features:

• Heavy duty lower buffer chamber
• Interlock safety lid with attached leads
• Gasketed (cast in place) 7cm wide x 8cm long UV-transparent gel tray with built-in fluorescent ruler and 2 comb slots.
• One 1.5mm x 6 tooth comb
• One 1.5mm x 10 tooth comb

View available accessories: RapiCast 0708 Accessories

View the Galileo Bioscience electrophoresis and blotting system catalog]]> System features The 0708 complete system includes these features:

• Heavy duty lower buffer chamber
• Interlock safety lid with attached leads
• Gasketed (cast in place) 7cm wide x 8cm long UV-transparent gel tray with built-in fluorescent ruler and 2 comb slots.
• One 1.5mm x 6 tooth comb
• One 1.5mm x 10 tooth comb

]]> 1749 0 34 0 https://nebg.biobm.com/?post_type=product&p=3830 Mon, 11 Mar 2024 08:53:16 +0000 https://nebg.biobm.com/?post_type=product&p=3830 Galileo Insight™ Semi-Dry Blotting Systems by Galileo bioscience Choose Galileo Insight™ semi-dry sytems for rapid and efficient molecular transfer Galileo semi-dry electroblotters provide quick and efficient transfer of nucleic acids and proteins from agarose or acrylamide gels. Since only the tranfer "sandwich" of gel, membrane and blotting papers must be kept wet, much less buffer is required than traditional tank transfer systems. Solid plate style electrodes (stainless steel cathode and platinum anode) assure even and complete molecular transfer.

G91-1010-SD System

Low cost and quick transfer times make this system is ideal for the rapid blotting of small pre-cast and self-cast gels. The 1010-SDBLT device will easily accomodate almost all pre-cast gels, and for many larger format pre-cast gels, once the stacking area of the gel is removed the "working" area of will also fit easily into the 1010-SDBLT device. System is supplied complete with power leads and a sample pack of our ultra-pure cotton fiber filter paper.

G91-2020-SD System

The larger format 2020 system is ideal for transfer of larger gels or simultaneous transfer of multiple smaller gels. System is supplied complete with power leads and a sample pack of our ultra-pure cotton fiber filter paper. View the Galileo Bioscience electrophoresis and blotting system catalog]]> 3830 0 24 0 https://nebg.biobm.com/?post_type=product&p=3839 Mon, 11 Mar 2024 09:44:04 +0000 https://nebg.biobm.com/?post_type=product&p=3839 0911 Mini Gel Unit by Galileo bioscience The Galileo 0911 is a mini-gel unit with extended run length for improved resolution. It also features a RapidCast gasketed tray for quick in-unit gel casting.

System description

The standard 0708 mini gel system includes a RapiCast™ gasketed gel tray for convenient gel casting right in the buffer chamber.

System features

The 0911 complete system includes these features:

• Heavy duty lower buffer chamber
• Interlock safety lid with attached leads
• Gasketed (cast in place) 9cm wide x 11cm long UV-transparent gel tray with built-in fluorescent ruler and 2 comb slots.
• One 1.5mm x 10 tooth comb
• One 1.5mm x 14 tooth comb

View available accessories: RapiCast 0911 Accessories

View the Galileo Bioscience electrophoresis and blotting system catalog]]> 3839 0 35 0 https://nebg.biobm.com/?post_type=product&p=3842 Mon, 11 Mar 2024 10:50:26 +0000 https://nebg.biobm.com/?post_type=product&p=3842 UltraBright blue light transilluminator for gels up to 20cm x 16cm (part number: NEB-LB-16). One year warranty and FREE SHIPPING! High quality at a reasonable price.

Use coupon code ULTRABRIGHT and save an additional $145.00 off your purchase. Special offer price $650.00. Once coupon code has been applied, the discount price and free shipping will display below.

Check out our UltraSlim Blue Light LED Transilluminator! Video demonstration of one of our blue light transilluminators:

For easy visualization of DNA gels stained with:

• SYBR Safe
• Ethidium Bromide (EtBr)
• Gel Green
• UltraPower DNA Safe Dye
• Midori Green

The Next Generation of Blue Light Transilluminators. Works with gels up to 20cm x 16cm.

New England BioGroup, LLC is  proud to offer the UltraBright-LED, a new generation of blue light transilluminator. The UltraBright-LED has two side-mounted high intensity LED arrays. This produces uniform and bright excitation at 470nm making it suitable for a wide range of dyes. The UltraBright-LED is compatible with the following dyes: GelGreen, SYBR Green, SYBR Gold, SYBR Safe, UltraSafe Blue and even ethidium bromide stained DNA gels. Gels up to 20 x 16cm can be viewed. The use of special filter material built into the lid of the UltraBright transilluminator greatly enhances the contrast of bands providing the optimum viewing conditions that are ideal for image capture and band cutting. UltraBright-LED is powered using a universal voltage, 24V power supply. The overall dimensions are 33.0W x 28.0Dx 9.0H cm. The UltraBright-LED can be used in a Syngene G:BOX or as a stand-alone unit.

Safe to use and does not damage DNA

Despite the increased interest in "safe/non-toxic" DNA gel stains such as SYBR Safe or GelGreen, many labs still use UV transilluminators on the bench.  Exposure to UV can cause sunburns to skin or eyes.  Additionally, UV can accidentally crosslink and damage your DNA.  As the UltraSlim, or UltraBright, units use blue light, there is no risk of sunburn or damage to DNA.  Simply put, this is a safer system to use on the bench.  To read more about safety of blue light transilluminators, please click here. Why buy this product

• High Sensitivity Using 224 high intensity LEDs, the UltraBright-LED illuminates a wide range of samples, producing fluorescence in even the faintest of bands. The illumination is high intensity and uniform, giving high quality images for publication or analysis.

• Uniformity of light distribution over the entire surface area  Our high intensity LED are placed equally on both sides of the unit (112 on each side), giving the instrument very intense and uniform light distribution over the entire surface area. Other blue light transilluminators use bulbs, or a very small array of LED bulbs which do not cover the entire surface of the viewing area.  As such, light may not evenly distributed, making bands appear dimmer than they may be or, even worse, faint bands may not be visualized.

• Small footprint The UltraBright-LED can be used as a stand-alone transilluminator and takes up very little laboratory bench space. Its overall dimensions are only 33cm x 28cm and it is easily portable to any location. The UltraBright-LED is compatible with any Syngene G:BOX system.

• Uses a wide range of dyes The UltraBright-LED transilluminator can be used with Ethidium bromide, GelGreen, SYBR Gold, SYBR Green, SYBRSafe, SYPRO Orange, SYPRO Ruby UltraSafe Blue. (All trademarks acknowledged).

Specification Dimension - 330(d) x 210(w) x 90(h)mm Gel size (mm) - 200 x 160 Wavelength (nm) - 470nm Power - DC 24v 0.65A Weight - 2.3kg]]> 3842 0 4 0 https://nebg.biobm.com/?post_type=product&p=3844 Mon, 11 Mar 2024 10:52:30 +0000 https://nebg.biobm.com/?post_type=product&p=3844 UltraSlim Blue LED transilluminator for gels up to 12cm x 10cm (part number: NEB-SLB-01W)- One year warranty and FREE SHIPPING! High quality at a reasonable price.

Use coupon code ULTRASLIM and save an additional $127.00 off your purchase. Special offer price $398.00. Once coupon code has been applied, the discount price and free shipping will display below

Need a bigger imaging area?  Check out our UltraBright blue light transilluminator! A video demonstration of the UltraSlim illuminating a DNA gel stained with SYBR Safe gel stain:

For easy visualization of DNA gels stained with:

• SYBR Safe
• Ethidium Bromide (EtBr)
• Gel Green
• UltraPower DNA Safe Dye
• Midori Green

High Intensity Blue Light Transilluminator with 108 LEDs.

This exciting product uses a high intensity LED array which can illuminate a range of dyes  including GelGreen, SYBRSafe, EtBr and the new UltraPower DNA safe dye. UltraSlim-LED provides a uniform and bright excitation across gels up to 10 x 12cm.

Even Illumination Over the ENTIRE Viewing Area

The UltaSlim is very compact and has two arrays of  54 high intensity 470nm blue LEDs. As the UltraSlim illuminates samples from the side, it provides a low signal to noise ratio (S/N) and extremely even illumination throughout the entire sample.  A built-in filter/lid provides the optimum viewing conditions and is ideal for band cutting. Some blue light transilluminators use bulbs, which create "peaks and valleys" of light intensity.  Others employ a very small array of LED bulbs mounted below the viewing area.  However, the LEDs do not cover the entire surface of the viewing area.  As such, light may not evenly distributed, making bands appear dimmer than they may be or, even worse, faint bands may not be visualized. All UltraSlim-LED units are powered using a universal voltage, 24V power supply. The overall dimensions are 21.0W x 21.0D x 3.0H cm. UltraSlim-LED can be used in any Syngene darkroom or as a stand-alone unit. (All trademarks acknowledged)

Safe to use and does not damage DNA

Despite the increased interest in "safe/non-toxic" DNA gel stains such as SYBR Safe or GelGreen, many labs still use UV transilluminators on the bench.  Exposure to UV can cause sunburns to skin or eyes.  Additionally, UV can accidentally crosslink and damage your DNA.  As the UltraSlim, or UltraBright, units use blue light, there is no risk of sunburn or damage to DNA.  Simply put, this is a safer system to use on the bench.  To read more about safety of blue light transilluminators, please click here.

Why buy this product?

1. No Separate Pieces.  No Need to Purchase or Share (eww!) Amber Viewing Glasses The UltraSlim has an attached variable position viewing screen/cover.  Should you wish to extract bands from gels, simply lift the cover to a comfortable viewing angle and it will remain in place.  The cover never touches your gels.  Additionally, you do not need to worry about losing a separate cover or glasses with the UltraSlim. 3. High Sensitivity Using 108 high intensity LEDs, the UltraSlim-LED illuminates a wide range of samples, producing fluorescence in even the faintest of bands. The illumination is high intensity and uniform, giving high quality images for publication or analysis. 4. Small footprint The UltraSlim-LED can be used as a stand-alone transilluminator and takes up very little laboratory bench space. Its overall dimensions are only 21 x 21cm and it is easily portable to any location. The UltraSlim-LED is compatible with any Syngene darkroom. 5. Uses a wide range of dyes The UltraSlim-LED can be used with Ethidium bromide, GelGreen, GelRed, SYBR Gold, SYBR Green, SYBRSafe, SYPRO Orange, SYPRO Ruby and UltraPower. (All trademarks acknowledged). Specifications: Dimension - 210(d) x 210(w) x 30mm Gel size (mm) - 100 x 120 Wavelength (mm) - 470nm Power - DC 24v 0.65A Weight - 1.3kg]]> 3844 0 5 0 https://nebg.biobm.com/?post_type=product&p=4130 Fri, 15 Mar 2024 15:56:01 +0000 https://nebg.biobm.com/?post_type=product&p=4130

WHAT ARE THE ADVANTAGES OF USING AUTOMATED ELISA SYSTEMS?

ELISA is probably the most widespread method, performed in microplates. The assay requires one or more steps of washing, dispensing, incubation, shaking and reading. It is widely used in research, food testing, diagnostics and other fields. There are several factors that make ELISA automation very desirable:

• While the operator can perform other tasks during incubations, manually performing the assay requires the operator to stay nearby for several hours and to abandon other tasks when the assay requires his/her attention.
• Both, the accurate timing of the incubation steps and the reproducibility of the washing process are very important for the consistency and reliability of the assay. These steps are difficult to keep constant with manual operation.
• Higher throughput laboratories that need to analyze many plates per day would need more staff to provide the manual power required to perform the ELISAs.

Automation solves all these problems by increasing reliability and reproducibility, increasing throughput, freeing staff from repetitive tasks and allowing them to focus on more important tasks. However, there are different forms of ELISA automation, so it is very important to choose the right instrument for the task.

TYPES OF AUTOMATED ELISA SYSTEMS

Most instruments on the market offering ELISA automation have been designed to analyze immunoassays in large hospitals. This kind of equipment is capable of performing a large quantity of different assays per day, but it is bulky, expensive and requires frequent and costly maintenance. In addition, ELISA kit manufacturers very often provide these instruments to support their applications. Thus, they have little flexibility to perform assays from other manufacturers, or are sold alongside an agreement to purchase a large quantity of kits during a given period of time. Hence, they are not always the best choice for customers other than large hospitals.

Automated ELISA systems by functions

All automated ELISA systems offer the basic functions needed to process an ELISA: reagent dispensing, microplate washing, incubation and absorbance measurement. But some other functions are not always available and, if you are looking to purchase a system for your laboratory, it is important to know if you require them or not:

• Sample handling: this includes sample dilution and sample transfer from tube to microplate. It often requires barcode reading for identification and tracking of samples. While this function can be very convenient if large quantities of samples have to be processed, it makes the system much more complex, and hence more expensive and maintenance-intensive.
• Measurement of additional labels: while absorbance is the most popular label used in immunoassays, some assays use chemiluminescence or fluorescence instead. This requires either that the automated system can measure those labels, or that the plate is moved to a suitable reader for quantification.
• Analysis of results: most ELISA kit manufacturers provide simple Excel spreadsheets to perform the calculations needed to get the results. For many users this is fully sufficient. However, in other cases more advanced software packages are required that enable a more complex analysis.

Automated ELISA systems by throughput

Another important distinction is the number of samples to be processed per day: low-throughput instruments are affordable and the perfect solution for laboratories processing only a few plates per day. On the other hand, higher throughput laboratories do need more sophisticated and expensive instrumentation to perform the job.

• Low throughput systems: these workstations are usually able to process a single plate per run only and often provide just very basic functionality. They offer a solution for laboratories moving from manual processing to automation for the first time, or performing a rather low number of ELISAs per day.
• Medium throughput systems: these are instruments prepared to hold 2 to 3 plates, and often include sample handling. They are the solution of choice for laboratories processing more than a few plates a day. An alternative to a single medium-throughput instrument is to use 2 or more low-throughput instruments working in parallel. The advantages are, that different workflows are sometimes easier to handle in parallel, and instruments can serve as backup, in case that one of them is down for maintenance or repairs.
• High throughput systems: these instruments can process simultaneously 4 or more plates (up to 16 is not uncommon) and include extensive sample handling capabilities. High-throughput Laboratories may need a custom automation or semiautomated solution enabling the required throughput.

]]> 4130 0 3 0 https://nebg.biobm.com/product/syngene-nugenius-and-nugenius-gel-documentation-system-2/ Fri, 23 Feb 2024 09:56:12 +0000 https://nebg.biobm.com/?post_type=product&p=1686 NuGenius is a new generation, low cost, integrated imaging system for DNA and protein analysis and gel documentation.

Complete gel documentation workstation

NuGenius is the ultimate in compact gel documentation workstations. Using a choice of UV, blue or white lighting options you can capture and edit images of fluorescent ethidium bromide, SYBR^® Safe or GelGreen^TM DNA gels and protein gels stained with Coomassie Blue at the workstation. You can even capture stain-free gel images using a NuGenius+ which is specially optimised for this application. For an entry level system, the NuGenius comes with a ground breaking 5 million pixel CCD camera giving superb pixel resolution and unrivalled sensitivity. NuGenius offers a maximum viewing area of 20 x 24cm which is very large for such a small, compact unit. NuGenius+ has been designed for stain free applications. NuGenius+ can be automatically set up in seconds to capture great images of TGX Stain-Free™ FastCast™ acrylamide gels, Mini-PROTEAN^® and Criterion™ TGX Stain-Free™ precast gels. Utilising this cost-effective system for imaging stain-free gels will save researchers hours of staining preparation time.

Features

Gel imaging at a touch. DNA and protein gel imaging your way!

• Compact workstation Plenty of bench space for your research
• Complete with built-in processor, touch screen and NuGenius software Easily capture and edit images on screen without a computer
• Motor driven zoom lens Automatic set up for easy image capture
• 5m pixel camera Resolves close bands and detects nanogram amounts
• White, UV and blue lighting options Flexibility to image fluorescent and visibly stained gels
• Stain-free imaging capability with the NuGenius+ Capture images of TGX Stain-Free™ FastCast™ acrylamide gels and many more
• Complete networking connectivity (optional) Easily share and store your results
• GeneTools software (unlimited copies) Analyse date on your own computer

Product specifications

NuGenius	NuGenius+
Camera	5 million pixel	5 million pixel
Sensor	1/2.5 inch	2/3 inch
Bit depth	12/16 bit	12/16 bit
Greyscale	65,536	65,536
Dynamic range	3.6/4.8 (extended)	3.6/4.8 (extended)
Lens	8 – 48mm f/1.2	11.5 – 69mm f/1.4
Viewing area	20 x 24cm	20 x 24cm
Illumination
Slim transilluminator 20 x 24cm	Option	Option
Blue converter screen 21 x 26cm	Option	Option
Visible light converter	Option	Option
White epi	Yes	Yes
7 inch touch screen
Software
Image capture	Yes	Yes
GeneTools analysis	Yes	Yes
GeneDirectory	Option	Option
Printer
	P95DW Mitsubishi digital thermal printer	P95DW Mitsubishi digital thermal printer
	Canon Selphy CP1200	Canon Selphy CP1200
Paper and ink	K65HM thermal paper, matt	K65HM thermal paper, matt
	K91HG thermal paper. glossy	K914HG thermal paper, glossy
	Canon KP-1081N	Canon KP-1081N
Dimensions
H x W x D cms	75 x 31 x 45	75 x 31 x 45
Weight	20kg	20kg

]]> Complete gel documentation workstation NuGenius is the ultimate in compact gel documentation workstations. Using a choice of UV, blue or white lighting options you can capture and edit images of fluorescent ethidium bromide, SYBR^® Safe or GelGreen^TM DNA gels and protein gels stained with Coomassie Blue at the workstation. You can even capture stain-free gel images using a NuGenius+ which is specially optimised for this application.]]> 1686 0 85 0 https://nebg.biobm.com/product/herm-lb500-with-flumo-detector-radio-hplc-detector/ Fri, 23 Feb 2024 12:32:51 +0000 https://nebg.biobm.com/?post_type=product&p=1750 HERM LB500 with fLumo detector for gamma and high energy beta isotopes The HERM is a dedicated radio HPLC monitor which can be connected to different detectors providing flexibility, reproducibility and sensitivity. The HERM with fLumo Chemiluminescence detector for HPLC systems uses a highly sensitive head-on photon counting detector with low background which is perfect for PET quality control, among other applications. Different measuring cells can be used to adapt for flow rates, activities and isotopes. For PET and direct luminescence, the HERM with Nal detector is available. Simple integration into every HPLC system The HERM makes integration into any existing HPLC system very easy. The PC connection can be established instantly via the USB port or by using the analog 0-1 V output. Its compact design makes it easy to fit in to a hot cell. Highly sensitive luminescence monitoring In the fLumo detector the low noise head-on PMT has been designed to face the sample coil directly. The result is a high sensitivity detector for even the faintest traces of chemiluminescence signals. Background-free Gamma and PET monitoring The use of specific measuring cells simplifies the measurement of high activity PET isotopes even in high background laboratories. A bulky and heavy lead shielding is not required as the system is insensitive to 511 keV gamma. Available cells include:

• PET measuring cells
• Luminescence measuring cells
• Gamma measuring cells

Dual operation mode The HERM system can be installed in multiple distinct configurations, providing more flexibility and convenience. The comfortable stand-alone configuration provides an analog signal which can be easily integrated into an existing HPLC system. The system is equipped with a flash memory and a powerful CPU. Alternatively, the system can be installed in the PC-controlled configuration via a USB connection for digital data acquisition. Easy-to-use software for maximum productivity The HERM Controller is the software interface to the instrument; it controls all functions and collects the data. The RadioStar software combines the ability to run sophisticated measurements with a user-friendly interface. A Chromeleon™ driver is available, Chromeleon™ is known as a very versatile chromatography software package supporting many different hardware solutions. The driver is fully certified and digitally signed by Thermo Scientific™ and runs with versions 6.x and 7.x. Specifications: Detection unit: Low noise photon counting 1 inch PMT Interface: USB port (mini USB connector) Inputs: Start signal (contact closure) Outputs: TTL signal output (raw data), analog signal 0-1 V with 16-bit resolution, 2 programmable relay outputs Software: The following options are available: 1. External control and evaluation via RadioStar software or 2. External control and evaluation via Chromeleon™ driver. Power Supply: 6 V DC (via wide range mains adapter) Dimensions (W x D x H): Electronics: 210 x 60 x 120 mm, fLumo detector: 225 x 150 x 130 mm Weight: Electronics: 1 kg, fLumo detector: 4 kg]]> 1750 0 21 0 https://nebg.biobm.com/product/herm-lb500-with-nal-detector-radio-hplc-detector/ Fri, 23 Feb 2024 12:33:25 +0000 https://nebg.biobm.com/?post_type=product&p=1752 HERM LB500 with Nal detector for gamma and high energy beta isotopes The HERM is a dedicated radio HPLC monitor which can be connected to different detectors providing flexibility, reproducibility and sensitivity. The HERM offers a safe and user-friendly solution for all quality control laboratories. The HERM with NaI detector for gamma and high energy beta isotopes combines an extremely sensitive NaI detector with compact shielding and can detect even low energy gamma isotopes like 125I with high efficiency. For PET and direct luminescence, the HERM with fLumo detector is available. Simple integration into every HPLC system The HERM makes integration into any existing HPLC system very easy. The PC connection can be established instantly via the USB port or by using the analog 0-1 V output. Its compact design makes it easy to fit in to a hot cell. Sensitive and reliable measurement The NaI detector option combines an extremely sensitive detector with a compact shielding. Even low energy gamma isotopes like 125I can be detected with high efficiency. Lead shielding ensures low backgrounds even when there is high external radiation. Easy operation and high flexibility The HERM LB 500 has been designed to be intuitive and easy to use. This will get you up and running as quickly as possible. Standard tubing is used to create the flow cell volume and provides maximum flexibility. Changing cells is very easy because of the twin cell changer that enables the user to easily switch between two different cell volumes. If required by GLP regulations, the cell volume can be fixed to just one volume using the optional single volume insert. The system has been designed to avoid accidental parameter modifications eliminating manual adjustment as all instrument parameters are controlled remotely. Dual operation mode The HERM system can be installed in multiple distinct configurations, providing more flexibility and convenience. The comfortable stand-alone configuration provides an analog signal which can be easily integrated into an existing HPLC system. The system is equipped with a flash memory and a powerful CPU. Alternatively, the system can be installed in the PC-controlled configuration via a USB connection for digital data acquisition. Easy-to-use software for maximum productivity The HERM Controller is the software interface to the instrument; it controls all functions and collects the data. The RadioStar software combines the ability to run sophisticated measurements with a user-friendly interface. A Chromeleon™ driver is available, Chromeleon™ is known as a very versatile chromatography software package supporting many different hardware solutions. The driver is fully certified and digitally signed by Thermo Scientific™ and runs with versions 6.x and 7.x. Specifications: Detection unit: State-of-the-art ultrasensitive PMT coupled 1 inch NaI detector Interface: USB port (mini USB connector) Outputs: TTL signal output (raw data), analog signal 0-1 V with 16-bit resolution, 2 programmable relay outputs Software: The following options are available: 1. External control and evaluation via RadioStar software or 2. External control and evaluation via Chromeleon™ driver. Power Supply: 6 V DC (via wide range mains adapter) Dimensions (W x D x H): Electronics: 210 x 60 x 120 mm, NaI detector: 140 x 180 x 100 mm, Lead shielding: 150 x 350 x 180 mm Weight: Electronics: 1 kg, NaI detector: 2 kg, Lead shielding: 25 kg.]]> 1752 0 22 0 https://nebg.biobm.com/product/flowstar%c2%b2-detector-for-radio-hplc-radio-hplc-detector/ Fri, 23 Feb 2024 12:34:27 +0000 https://nebg.biobm.com/?post_type=product&p=1754 Maximum Flexibility, Outstanding Sensitivity In the FlowStar² LB 514 ultimate sensitivity has been combined with touch-screen technology for quick and easy operation. Designed for maximum ease of use, safety and flexibility, the FlowStar² LB 514 sets the new standard for what a radio-HPLC flow detector should be. With over 50 years of experience Berthold Technologies is taking radio-HPLC flow detection to the next level. A wide range of available cells ensures optimal performance and supports full compatibility with both, routine and challenging applications. The convenient dual mode capability enables simple installation in different configurations and makes integration into existing systems easy. User friendly The FlowStar² LB 514 is designed to be intuitive and easy to use, to get you up and running quickly. The intuitive touch screen interface makes it easy to get started and keep going. Results are displayed in both, a graphical chromatogram and a numerical format, so you can start interpreting your results in real time while the run is still in progress. The FlowStar² frees you from configuring parameters manually, thanks to the use of SmartCells: all measuring cells are equipped with a chip to automatically set the correct cell-specific system parameters. In addition, the software includes an extensive isotope library with predefined energy windows and cell usage options (14C, 3H, 32P, 33P, 35S, 128I, 111In, 99mTc…). Easy integration into your HPLC system The FlowStar² LB 514 makes integration easy so that everything works together to ensure consistent and reliable results. The integrated A/D converter records signals from external instruments (e.g. UV-detector) without requiring expensive additional hardware, while the USB port enables external control using RadioStar or other HPLC control software (e.g. Chromeleon™). In addition, the dual analog output allows for the convenient integration into existing HPLC data systems. The FlowStar² supports UHPLC and other LC technologies:

• UHPLC
• HPLC-MS (Mass Spectrometry)
• HPLC-NMR (Nuclear Magnetic Resonance)

Safe and secure

The FlowStar² LB 514 embodies our commitment to providing everything you need for safe and easy integration into your lab environment.

Safety features:

• Automatic HV shutdown for system safety.
• Built-in leak detection to prevent the measuring system from damage caused by aggressive liquids. The system will shut down automatically in the event of cell leakage.

Security features:

• On-board system performance tests with instrument-based history function.
• Password-based multi user access control to protect instrument from unauthorised use.

Validation:

Several test cells are available to perform periodic system performance tests. These cells have been developed to meet GLP/GMP requirements and to guarantee a continuous system performance. Contact us for more information.

High sensitivity

The FlowStar² is the ultimate platform for high-sensitivity radio-HPLC flow detection. It uses random coincidence counting and luminescence subtraction for ultimate performance. In addition, the integrated spectrum scanning feature provides improved optimization of detector setup for each isotope.

Easy-to-use software for maximum productivity

The RadioStar software combines the ability to run sophisticated measurements with a user-friendly operation and user interface. In addition, a Chromeleon™ driver is available. Chromeleon™ is known as a very versatile chromatograpy software package supporting many different hardware solutions. The driver is fully certified, digitally signed by Thermo Scientific™ and runs with versions 6.x and 7.x

Measuring cells specific to your needs

Choose from a wide range of available SmartCells to meet your application needs. These high sensitivity cells are equipped with a SmartChip to automatically set the correct cell-specific system parameters. The automatic shut-down feature of the system after removal of a cell from the detector provides additional safety. The range of SmartCells include:

• Liquid scintillation (admixture) cells
• Solid scintillator cells
• Gamma isotopes and Positron Emitter (PET) cells
• Cells for preparative SFC
• Microbore cells for UHPLC and UPLC™
• Special Iodine Cells (125I)
• Cherenkov cells for high energy beta emitters

RADIO HPLC CELL CONFIGURATION AND APPLICATION FINDER

Specifications

Detection unit: State-of-the-art ultrasensitive dual 2“ PMT detection system with random coincidence counting and luminescence subtraction. Display: Graphical touch screen with 320 x 240 pixel resolution. Interface: USB port (B-type), RS-232 for pump control Inputs: Start-, Stop- and Ready signal (TTL). 2 analog inputs (24 bit resolution), variable voltage (bipolar, max 2.5 V) Outputs: Scintillator pump control (RS-232 and analog). 2 analog signal outputs 0-1 V (2,5 times oversampling) with 16-bit resolution. Waste valve and fraction collector control output (open collector output). Power Supply: 90-264 V, 50/60 Hz Dimensions (W x D x H): 410 x 170 x 410 mm. Horizontal and vertical operation possible. Weight: 16kg Operating Temperature: 10-35°C Storage Temperature: 5-40°C Humidity: 10-90% (non condensing)

Software: The following possibilities are available:

• Built-in software operated with touch screen
• External control and evaluation via RadioStar software
• External control and evaluation via Chromeleon™ driver.

]]> Specifications

Detection unit: State-of-the-art ultrasensitive dual 2“ PMT detection system with random coincidence counting and luminescence subtraction. Display: Graphical touch screen with 320 x 240 pixel resolution. Interface: USB port (B-type), RS-232 for pump control Inputs: Start-, Stop- and Ready signal (TTL). 2 analog inputs (24 bit resolution), variable voltage (bipolar, max 2.5 V) Outputs: Scintillator pump control (RS-232 and analog). 2 analog signal outputs 0-1 V (2,5 times oversampling) with 16-bit resolution. Waste valve and fraction collector control output (open collector output). Power Supply: 90-264 V, 50/60 Hz Dimensions (W x D x H): 410 x 170 x 410 mm. Horizontal and vertical operation possible. Weight: 16kg Operating Temperature: 10-35°C Storage Temperature: 5-40°C Humidity: 10-90% (non condensing)

]]> 1754 0 20 0 https://nebg.biobm.com/product/celena-s-digital-cell-imaging-system-digital-microscope/ Tue, 27 Feb 2024 07:44:19 +0000 https://nebg.biobm.com/?post_type=product&p=2059 CELENA® S Digital Imaging System

Multicolor fluorescence imaging to data analysis in one device

The CELENA® S Digital Imaging System makes capturing high resolution, publication-quality images a breeze. Don’t let its size fool you, the CELENA® S is equipped with advanced precision optics, a highly sensitive CMOS sensor, digitally controlled LED light sources with hard-coated fluorescence filters, and a computer with image analysis software. The sophisticated yet simple software supports multicolor fluorescence imaging, brightfield imaging, phase contrast imaging, live cell time lapse imaging, and Z-stack imaging.

The All-In-One System for Exceptional Imaging Quality

  VIBRANT, PUBLICATION-QUALITY IMAGES Advanced precision optics and a highly sensitive camera allow you to capture high resolution, multi-channel fluorescence images. LIVE CELL TIME LAPSE IMAGING Live cells can be imaged in time lapse mode in a precisely controlled environment. The images can be used to create a video with annotations. CELL COUNTING Check cell concentration and viability in cell counter mode. The CELENA® S automatically adjusts light and focus for optimal results. INTERCHANGEABLE FILTER CUBES AND OBJECTIVES 12+ hard-coated LED fluorescence filters. Infinity-corrected objectives with a magnification range of 1.25-100X

LIVE CELL IMAGING MADE SIMPLE WITH AN ONSTAGE INCUBATOR

The CELENA® S onstage incubation system can be set up for a variety of live cell imaging experiments in physiological and non-physiological conditions. The gas mixer allows precise control over the composition, humidity, and temperature of the air. The temperature controller heats the plate and lid of the stage incubator separately, for increased control over the temperature of the system as well as preventing condensation formation. The CELENA® S makes it easy to set up a time lapse imaging sequence for lengthy cell-based assays, as well as exporting images or creating time-lapse videos complete with annotations. A collection of vessel holders are available to accommodate various flasks, dishes, plates, and slides. Download vessel holder selection guide Objective Selection Guide Our objectives provide excellent resolution, high contrast imaging, and color correction. Download lens objective selection guide LED Filter Cube Selection Guide Download our LED filter cube selection guide to find which filter cubes fit your needs the best. Download LED filter cube selection guide

How to use the CELENA-S

Capturing perfect images

Image analysis

Parfocal correction

Focus memory and fine AF

Z-stack imaging

Focus stacking images

]]> 2059 0 7 0 https://nebg.biobm.com/product/luna-fl-fluorescent-and-bright-field-automated-cell-counter/ Tue, 27 Feb 2024 07:59:07 +0000 https://nebg.biobm.com/?post_type=product&p=2068 Download brochure                                 contact us

An fluorescent automated cell counter has never been this easy or affordable.

• Dual fluorescence optics for sensitive cell analysis
• Trypan blue stained cell counting with bright field optics
• Unmatched cell counting accuracy
• Optimized for primary cells, stem cells, PBMCs, splenocytes, etc.
• Most affordable counting cost
• Cell size gatingInteractive graphic user interface

Dual Fluorescence Cell Counting

The LUNA-FL™ Dual Fluorescence Cell Counter from Logos Biosystems is a quantum leap forward for automated cell counting and cell viability analysis. The LUNA-FL™ Dual Fluorescence Cell Counter gives you sensitive and accurate live/dead counts without  cell type restrictions. Unlike immortalized cell lines, primary cells such as PBMCs, splenocytes, neutrophils, and stem cells have been difficult to count with conventional cell counters such as the Coulter counter or automated image-based cell counters utilizing  bright field optics. Primary cells are often contaminated with undesirable debris that can be miscounted as cells with conventional  cell counters. The LUNA-FL™ is equipped with dual fluorescence optics to overcome this problem. Live and dead cells are stained  with green and red fluorescence dye, respectively, and subsequent cell images are analyzed with accurate image analyzing software

To read a general review of fluorescent cell counting, please click here.

Bright Field Optics For Trypan Blue Cell Counting

The LUNA-FL™ has inherited the proven performance of the LUNA™. The precision bright field optics of the LUNA™ has been integrated into the LUNA-FL™ to provide the convenience of trypan blue cell counting. The powerful and precise cell counting  algorithm of the LUNA™ is used in the LUNA-FL™ LUNA-FL™ = LUNA™ + Dual fluorescence cell counter

Cell Counting Accuracy: Declustering

The LUNA-II™ automated cell counting algorithm has been recognized to have the best-in-class cell counting accuracy. The LUNA™ software has exceptional acc

 

uracy in cell de-clustering and can successfully count clumpy cells. The LUNA-II™ automated cell counter has inherited this well-known performance aspect of the LUNA™ software. Clumpy cells are de-clustered quickly and automatically, then counted as individual cells.

GFP Transfection Efficiency Assay

With the LUNA-FL™ Dual Fluorescence Cell Counter, GFP expression analysis takes less than 30 seconds. Bright field and fluorescence cell images are analyzed to detect GFP-positive and negative cells, and a corresponding fluorescence intensity histogram is displayed for further analysis. The LUNA-FL™ Dual Fluorescence Cell Counter is integrated with on-board flow cytometry type gating software. Be confident in the accuracy of your results by adjusting the threshold of fluorescence intensity.

Reduced Running Cost

Automated cell counters utilize disposable counting slides to eliminate washing steps of manual cell counting with the glass hemocytometer. Although disposable cell counting slides have several advantages, the increased running cost has been a substantial concern. Logos Biosystems developed a patented T-BOND technology to manufacture precision cell counting slides more efficiently. Therefore, the unit price of counting slides became much more affordable, providing significant cost savings.

Compact and Fast Thermal Printer (optional)

LUNA-FL™ provides a compact mobile printer for further record keeping purpose. Thermal roll paper or label paper can be selected. This plug & play mobile printer is ready-to-use after the USB to USB connection.

Interactive Software Interface

Options for  staining methods

The LUNA-II™ automated cell counter can count samples with or without trypan blue dye. The selection of trypan blue presence or absence can easily be chosen in the settings menu.

Cell size histogram and cell size based gating

After counting, cells can be gated based on their size information provided by the LUNA-II™. The specific size of cell populations Can easily be included or excluded on histograms.

Cell cluster map

Counted cells can be sorted with cell cluster map, into single cells, doublets, or triplets. The cell cluster map can be used to monitor changes in culture conditions or cell isolation/preparation protocols.

Review and Re-analysis option

The LUNA-II™ automated cell counter provides a powerful review option. A separate PC unnecessary. Users can open cell images directly on the LUNA-II™ automated cell counter to check previous results.

Various file save options

The LUNA-II™ can store up to 1,000 counts onboard. After counting, the result is automatically saved in the memory, and previous count results can be exported via USB as a .CSV file for further analysis. The results and image data can be saved in three different file formats such as TIF, annotated TIF, or a PDF report file. The PDF report file contains all the data generated during/after the cell counting, i.e., the protocol used, cell counting results, date, raw/analyzed cell image(s), and histograms are included in the PDF report file.]]>

Dual fluorescence optics for sensitive cell analysis

Trypan blue stained cell counting with bright field optics

Unmatched cell counting accuracy

Optimized for primary cells, stem cells, PBMCs, splenocytes, etc.

Most affordable counting cost

Cell size gatingInteractive graphic user interface

]]> 2068 0 30 0 https://nebg.biobm.com/product/dsc-400-single-cell-dissociator-tissue-dissociator/ Tue, 27 Feb 2024 08:05:35 +0000 https://nebg.biobm.com/?post_type=product&p=2076 DSC-400 Single Cell Suspension Tissue Dissociator. Heating Included! Process up to 4 samples at once. The smart way to to dissociate single-cell suspension from human/mouse/rat tissue. The DSC-400 is designed to help you quickly prepare tissue into a single cell suspension with high cell viability or tissue homogenate, greatly increasing experimental repeatability. Independent four channels with automatic processing improve the efficiency of organization processing. The DSC-400 single-cell suspension dissociator uses tissue processing tubes, tissue dissociation kits, and built-in optimization programs for preparing high-activity single-cell suspension and tissue homogenate. The preparation of single-cell suspension with high cell viability can be completed within 15-30 minutes, which makes the experiments more reproducible. 4 independent operating channels and heating jackets can automate the processes and improve the efficiency of tissue processing. Heating jackets are available for fully automated sample preparation. Applications

• Compound toxicity evaluation
• Drug development process
• Single-cell sequencing
• Primary cell culture
• Fluorescence-activated cell sorting
• Cell therapy
• Flow cytometry

Tissue processing tube specifications:

• Ethylene oxide(EO) sterilized
• Independent packaging makes it convenient for storage and use

The DSC-400 is easy to you and its color touchscreen and user-friendly software provides one-touch to run preset processing programs or edit custom programs.  It has 4 independent working channels and each channel can run different programs simultaneously, and it can also be equipped with a heating jacket to achieve automatic sample preparation. Sable motor speed ranges from 0 to 4000 rpm, capable of single-cell suspension and tissue homogenate. Customized tissue dissociation kits and tissue processing tubes can ensure cell viability and reduce experimental losses. Specifications:

Model	DSC-400
Sample volume	20-4000mg
Buffer volume	0.3-10mL
Speed	0-4000 rpm
Temperature	37℃
Channels	4
Operation method	Touch screen
Dimensions (W*D*H)	215*380*190 mm
Weight	~10.3 kg

Download brochure: here]]> 2076 0 13 0 https://nebg.biobm.com/product/lumat-lb-9510-luminometer-for-tubes-tube-luminometer/ Tue, 27 Feb 2024 08:11:59 +0000 https://nebg.biobm.com/?post_type=product&p=2078 ULTRASENSITIVE. RELIABLE. COMFORTABLE. The Lumat LB 9510 offers the functionality, versatility and superior performance you can expect from a luminometer developed by Berthold Technologies. The optimized optical system provides true single photon counting combined with a low-noise photomultiplier tube for up to 6 decades of linear dynamic range. The new Lumat has all the key features needed for today’s research applications.

Ready to meet your application needs

The new Lumat is a tube luminometer that uses state-of-the-art technology based on more than 40 years of experience engineering and designing luminometers. The luminometer supports all basic luminescence technologies including:

• Flash-type Luminescence
• Glow-type Luminescence
• Bio-Luminescence
• Chemi-Luminescence

Luminescence detection at its best

Thanks to a stringent selection of photomultipliers working in photon counting mode and more than 40 years of experience building luminometers, the new Lumat offers excellent luminescence sensitivity: <1 zmol firefly luciferase and <1 amol ATP/tube (high sensitivity model) can be reliably detected.

JET injectors - highest accuracy, speed and cell-friendliness

The Lumat can be equipped with up to 2 JET injectors with variable volumes. The ultra-fast injection speed of JET-injectors eliminates any delay between injection and measurement and is critical to measure fast reactions, e.g. for flash luminescence measurements. Berthold Technologies' JET injectors use Teflon bellows for accurate and fast injections and guarantee most efficient mixing as well as extreme longevity.

• Accurate pipetting of small volumina: regardless of whether you want to inject large or small volumes, JET injectors ensure high accuracy and more than 98% accuracy over the entire volume range
• Low maintenance costs: frictionless operation for extended lifetime
• Cell-friendly performance: cell-friendly materials and negligible shear forces enable worry-free injection of cell suspensions, e.g. in Aequorin-based calcium assays

Convenient sample switching mechanism

The measurement of individual samples one after the other can be time-consuming. The Lumat has a turret mechanism that makes this job easier and increases your throughput. Thanks to its motor-driven revolving magazine mechanism based on a rotating chamber for two tubes (one for measuring and one for loading and unloading), so you can prepare and insert the next sample while the previous one is still measuring.

Powerful software

LightCompass® is a flexible software enabling easy data acquisition, analysis and reporting using Berthold Technologies' instruments. It has been optimized for different microplate and tube formats. LightCompass® features all the functions you can expect from an advanced scientific software, such as flexible creation of measurement protocols, curve fitting, qualitative analysis and compliance with FDA 21 CFR Part 11.]]> luminometer developed by Berthold Technologies. The optimized optical system provides true single photon counting combined with a low-noise photomultiplier tube for up to 6 decades of linear dynamic range. The new Lumat has all the key features needed for today’s research applications.]]> 2078 0 26 0 https://nebg.biobm.com/product/dsc-800-single-cell-dissociatortissue-do/ Tue, 27 Feb 2024 08:46:39 +0000 https://nebg.biobm.com/?post_type=product&p=2085 DSC-800 Single Cell Suspension Tissue Dissociator - Includes Heating! Process up to 8 samples at once!  A smarter way to dissociate single-cell suspension from human/mouse/rat tissue. RWD Life Science DSC-800 is designed to help you quickly prepare tissue into a single cell suspension with high cell viability or tissue homogenate, greatly increasing experimental repeatability. Independent 8 channels with automatic processing improve the efficiency of organization processing. RWD Single-cell suspension dissociator uses tissue processing tubes, tissue dissociation kits, and built-in optimization programs for preparing high-activity single-cell suspension and tissue homogenate. The preparation of single-cell suspension with high cell viability can be completed within 15-30 minutes, which makes the experiments more reproducible. 8 independent operating channels and heating jackets can automate the processes and improve the efficiency of tissue processing. Heating jackets are included as standard for fully automated sample preparation. Applications

• Compound toxicity evaluation
• Drug development process
• Single-cell sequencing
• Primary cell culture
• Fluorescence-activated cell sorting
• Cell therapy
• Flow cytometry

Download brochure here. The DSC-800 is easy to you and its color touchscreen and user-friendly software provides one-touch to run preset processing programs or edit custom programs.  It has 8 independent working channels and each channel can run different programs simultaneously, and it can also be equipped with a heating jacket to achieve automatic sample preparation. Sable motor speed ranges from 0 to 4000 rpm, capable of single-cell suspension and tissue homogenate. Customized tissue dissociation kits and tissue processing tubes can ensure cell viability and reduce experimental losses. Specifications:

Model	DSC-800
Sample volume	20-4000mg
Buffer volume	0.3-10mL
Speed	0-4000 rpm
Temperature	37℃
Channels	8
Operation method	Touch screen
Dimensions (W*D*H)	415*381*256 mm
Weight	18 kg

  Download brochure: here]]> 2085 0 14 0 https://nebg.biobm.com/product/luna-ii-automated-cell-counter/ Tue, 27 Feb 2024 08:51:10 +0000 https://nebg.biobm.com/?post_type=product&p=2090 Download brochure                                 contact us

An automated cell counter has never been this easy or affordable.

• Fully automated cell counting and cell viability analysis.
• Autofocus technology for fast and reliable cell counting.
• Trypan blue stained cell counting or non-stained cell counting methods.
• Various histograms and cell-size gating.
• Unmatched counting accuracy and speed.
• Built-in computer and printer.
• The most affordable running cost currently on the market. Don't spend more money and waste time on slides that require you to peel off a protective film! 

The Luna-II automated cell counter is the most advanced cell counter with unmatched speed, accuracy, and consistency of measurement. It is a stand-alone instrument integrating precision microscopy optics, an onboard PC, image analysis software, automatic focusing, and an optional built-in printer. The Luna-II accurately detects total/live/dead cells at concentrations ranging from 5x10-4 cells/ml to 1x10-7 cells/ml and cell sizes between 3um and 60um.

NEW AUTOFOCUSING TECHNOLOGY

The LUNA-II™ automated cell counter has integrated a novel focusing mechanism based on liquid lens technology. The liquid lens does not use any mechanical moving parts to change the Z stage. Instead, the Z position of the sample is rapidly obtained by the application of a small voltage to the liquid lens. The elimination of moving parts removes noise and significantly reduces the need for servicing. Even after multi-million cycles of focusing, the liquid lens does not require repair or service. In combination with this novel focusing mechanism, the LUNA-II™ has integrated a new autofocusing algorithm optimized for cell counting.

Cell Counting Accuracy: Declustering

The LUNA-II™ automated cell counting algorithm has been recognized to have the best-in-class cell counting accuracy. The LUNA™ software has exceptional accuracy in cell de-clustering and can successfully count clumpy cells. The LUNA-II™ automated cell counter has inherited this well-known performance aspect of the LUNA™ software. Clumpy cells are de-clustered quickly and automatically, then counted as individual cells.

Reduced Running Cost

Automated cell counters utilize disposable counting slides to eliminate washing steps of manual cell counting with the glass hemocytometer. Although disposable cell counting slides have several advantages, the increased running cost has been a substantial concern. Logos Biosystems developed a patented T-BOND technology to manufacture precision cell counting slides more efficiently. Therefore, the unit price of counting slides became much more affordable, providing significant cost savings.

Built-in Thermal Printer (optional)

A thermal printer is integrated in the LUNA-II™ automated cell counter, and the counting results can be printed immediately for record keeping purposes. Because the printer is already integrated in the LUNA-II™ automated cell counter, an additional cable connection is not required.

Interactive Software Interface

Options for  staining methods

The LUNA-II™ automated cell counter can count samples with or without trypan blue dye. The selection of trypan blue presence or absence can easily be chosen in the settings menu.

Cell size histogram and cell size based gating

After counting, cells can be gated based on their size information provided by the LUNA-II™. The specific size of cell populations Can easily be included or excluded on histograms.

Cell cluster map

Counted cells can be sorted with cell cluster map, into single cells, doublets, or triplets. The cell cluster map can be used to monitor changes in culture conditions or cell isolation/preparation protocols.

Review and Re-analysis option

The LUNA-II™ automated cell counter provides a powerful review option. A separate PC unnecessary. Users can open cell images directly on the LUNA-II™ automated cell counter to check previous results.

Various file save options

The LUNA-II™ can store up to 1,000 counts onboard. After counting, the result is automatically saved in the memory, and previous count results can be exported via USB as a .CSV file for further analysis. The results and image data can be saved in three different file formats such as TIF, annotated TIF, or a PDF report file. The PDF report file contains all the data generated during/after the cell counting, i.e., the protocol used, cell counting results, date, raw/analyzed cell image(s), and histograms are included in the PDF report file.]]> 2090 0 29 0 https://nebg.biobm.com/product/centro-microplate-luminometer-microplate-reader/ Tue, 27 Feb 2024 08:58:42 +0000 https://nebg.biobm.com/?post_type=product&p=2095 ULTRASENSITIVE, RELIABLE AND ACCURATE LUMINESCENCE MEASUREMENT The Centro is a high-performance, easy-to-use and versatile microplate luminometer for both, glow and flash luminescence assays. The optimized optical system of the instrument provides lowest background and negligible crosstalk (10^-6). It can be equipped with up to 3 injectors. The Centro XS version of the system has been built on the basis of the successful Centro, and shares with it its reliability and robustness, but has been optimized to increase its sensitivity even further: the Centro XS is the most sensitive microplate luminometer available and reliably measures less than 1.8 amol ATP per well. This makes the Centro XS the ideal partner for applications such as determination of cell viability, e.g. in tumour chemosensitivity assay, cell proliferation, antibiotic susceptibility testing or pathogen detection. The Centro supports all basic luminescence technologies including:

• Flash-type Luminescence
• Glow-type Luminescence
• Cellular Luminescence
• Bio-Luminescence
• Chemi-Luminescence

Sensitive and reliable

Thanks to a stringent selection of photomultipliers working in photon counting mode and more than 40 years of experience building luminometers, the Centro provides highly sensitive luminescence detection: less than 5 amol ATP/well (25 fM) can be detected reliably. In the Centro XS, optics have been optimized to increase its sensitivity even further: below 1.8 amol ATP/well (9 fM).

Powerful crosstalk control – reliable results

Do you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples?  Then you´re potentially affected by a phenomenon called crosstalk, that can produce wrong results and, hence, wrong conclusions. The crosstalk reduction design of the Centro does almost eliminate this problem: with a crosstalk of 10^-6, you never have to worry about the position of your high-intensity samples in your plate.

Integrated waste pump

Thanks to its built-in waste pump, you don’t need to worry about loading microplates, waste containers or removal of injector tips for washing and filling the reagent lines; the built-in waste pump takes care of the safe removal of excess liquids.

JET injectors: highest accuracy, speed and cell-friendliness

The Centro can be equipped with up to 3 JET injectors with variable volumes in pre-measurement or measurement position*. The ultra-fast injection speed of JET injectors eliminates any delay between injection and measurement and enables measurement of fast reactions, e.g. flash luminescence. Berthold Technologies' JET injectors use Teflon bellows for accurate and fast injections and guarantee most efficient mixing as well as extreme longevity.

• Accurate pipetting of small volumina: JET injectors ensure the injection of large and small volumes with high accuracy and offer a precision of more than 98% over the entire volume range
• Low maintenance costs: frictionless operation for extended lifetime
• Cell-friendly performance: cell-friendly materials and negligible shear forces enable worry-free injection of cell suspensions, e.g. in Aequorin-based calcium assays

* All injectors in pre-measurement position in the Centro XS.

Convenient Software options

Our microplate readers are powered by LightCompass®, a flexible software enabling easy data acquisition, analysis and reporting using Berthold Technologies' instruments. It has been optimized for different microplate and tube formats. LightCompass® features all the functions you can expect from an advanced scientific software, such as flexible creation of measurement protocols, curve fitting, qualitative analysis and compliance with FDA 21 CFR Part 11. LightCompass® is available in 3 different versions: Basic, Plus and Professional. Just pick the version that better suits your research needs.

TECHNICAL DETAILS

Detectors: Low-noise photomultiplier tube in single photon counting mode Detection technologies: Luminescence Measurement modes: Endpoint, Kinetics, Repeated, Area scan (up to 100 x 100 points per well) Spectral range luminescence: 340-630 nm Injectors: Up to 3 JET injectors with frictionless operation Injector volume: 10-100 µL in 1 µL steps Injector precision: Better than 2% across the entire volume range Injector accuracy: Better than 2% across the entire volume range Microplate formats: 96- and 384-well*  *Centro  XS only Shaking: From ambient +5° to 45° C (not available in Centro XS) Temperature control: From ambient +5° to 45° C (not available in Centro XS) Luminescence sensitivity: Centro: <5 amol (25 fM) ATP/well (96-well plate)/Centro XS: <1.8 amol (9 fM) ATP/well (96-well plate) Crosstalk: 10^-6 Dynamic range: >6 orders of magnitude Interface: Windows software, connected to computer via USB Operating system: Windows 10 Available software packages: LightCompass®: flexible software for data acquisition, analysis and reporting. Regulations: CE, NRTL Power Supply: 100 – 240 VAC ±10%, 50/60 Hz Operating Voltage: 24 VDC / 6.67 A Temperature tolerance: Storage: 0-40° C, Operation: 15-35° C Humidity tolerance: 10-80% non-condensing, decreasing linearly to 50 %, relative humidity up to 40 °C, decreasing linearly to 50 %, relative humidity up to 40 °C Dimensions (W x D x H): 334 x 437 x 287 mm Weight: 21.8 kg]]>

Flash-type Luminescence

Glow-type Luminescence

Cellular Luminescence

Bio-Luminescence

Chemi-Luminescence

]]> 2095 0 8 0 https://nebg.biobm.com/product/celena-xhigh-content-imaging-system/ Mon, 11 Mar 2024 09:00:54 +0000 https://nebg.biobm.com/?post_type=product&p=3832 Automated high content image acquisition and analysis for drug discovery and cell biology

The CELENA® X High Content Imaging System is an integrated imaging system designed for rapid, high content image acquisition and analysis. Customizable imaging protocols, image-based and laser autofocusing modules, and a motorized XYZ stage simplify well plate imaging and slide scanning. The integrated CELENA® X Cell Analyzer software processes images and data for quantitative analysis. Analysis pipelines can be put together and reused to identify cellular or subcellular objects, process images for optimal data collection, and make various measurements. The CELENA® X is as flexible as it is powerful, with interchangeable objectives and filter cubes to accommodate a wide range of fixed and live cell imaging applications.

Features

Fully automated plate and slide imaging

Automated vessel handling and scanning Motorized XYZ stage, filter cube stage, and objective turret

Laser Autofocus Rapid and reproducible focusing Minimized phototoxicity and photobleaching

Live Cell Assay Support Onstage incubation system for a variety of experiments in physiological and non-physiological conditions

Four Imaging Modes Fluorescence imaging in four channels, brightfield, color brightfield, and phase contrast imaging

Powerful, Easy-To-Use User Interface Simple setup of imaging protocols Seamless integration of imaging and data analysis processesCustomizable High Content Analysis

Create and customize image analysis projects Quantitatively analyze multiple image-based phenotypes

Create Your Own Imaging And Analysis Workflows With The CELENA

Imaging Raw images Metadata

Image Analysis Morphology of individual cells and organelles Spatial distribution of targets Multiple measurements per cell Differentiation of multiple phenotypes

Analyzed images Result files Graphs (coming soon)

Applications

• Cell Counting
• Cytotoxicity_BF
• Transfection Efficiency
• Wound Healing
• Apoptosis
• Cytotoxicity_FL
• Confluency
• Calcium Signaling
• Phagocytosis
• Cell Cycle
• Stitching
• 3D Cell Models

Versatile And Customizable For Your Cell Imaging Needs

Vessel Holder Selection Guide A collection of vessel holders are available to accommodate various flasks, dishes, plates, and slides.

Objective Selection Guide Download our objective selection guide to see the Olympus objectives we recommend for the CELENA® X.LED Filter Cube Selection Guide Download our LED filter cube selection guide to find which filter cubes fit your needs the best.

]]> Fully automated plate and slide imaging

Automated vessel handling and scanning Motorized XYZ stage, filter cube stage, and objective turret

Laser Autofocus Rapid and reproducible focusing Minimized phototoxicity and photobleaching

Live Cell Assay Support Onstage incubation system for a variety of experiments in physiological and non-physiological conditions

]]> 3832 0 6 0 https://nebg.biobm.com/product/luna-fx7-automated-cell-counter/ Fri, 15 Mar 2024 08:20:23 +0000 https://nebg.biobm.com/?post_type=product&p=4079 The End To The Accuracy Debate

The effect of cell counting volume on accuracy is often overlooked. Manual counting and most au

tomated cell counters detect cells in a counting volume of ~ 0.5 µl. The LUNA-FX7™ utilizes a counting volume of up to 5.1 µl, lowering error and CV for each count. Improved de-clustering algorithms and precise autofocus further add to the LUNA-FX7™’s accuracy and reliability.

 

Easy, Flexible And Robust

Not only the simple and efficient operation via intuitive touchscreen controls, brightfield and dual fluorescent detection options, multiple counting modes, and customizable protocols allow you to easily optimize for your cell and sample type. Counting results may be exported as a CSV summary or a comprehensive PDF report with raw and tagged images. With an internal 250GB hard drive, no additional computer is needed and data may be exported via Wi-Fi, Ethernet, or USB flash drive.

Unique Bioprocess Mode

A new bioprocess feature allows you to monitor, record, and analyze your different bioprocessing activities individually. Growth rates and trend charts may be produced that enable you to trace production and accurately forecast timelines.

Trust Your Data

The LUNA-FX7™ validation slides and QC software allow you to be confident and trust in your data. The brightfield and fluorescent validation slides offer exact cell concentration and viability while the QC features provide the ability to store, monitor, and analyze quality control data.

 ]]> 4079 0 28 0 https://nebg.biobm.com/product/x-clarity-systems-reagents-for-tissue-clearing/ Fri, 15 Mar 2024 10:42:54 +0000 https://nebg.biobm.com/?post_type=product&p=4103

The X-CLARITY™ systems and reagents standardize, simplify, and accelerate each step of the tissue clearing process. With the CLARITY method, preserved tissues are embedded in a hydrogel matrix and lipids are actively extracted through electrophoresis to create a stable and optically transparent tissue-hydrogel hybrid that is chemically accessible for multiple rounds of antibody labeling and imaging. Native cytoarchitecture remains intact and even endogenous fluorescence proteins are preserved for robust fluorescence imaging downstream.

X-CLARITY™ Polymerization System

The X-CLARITY™ Polymerization System is a standalone, automated system developed to simplify tissue-hydrogel hybridization, a crucial step for optimal tissue clearing. Multiple samples can be placed in multi-well plates or conical tubes for rapid and efficient high-throughput sample processing. Users can control polymerization by adjusting vacuum strength, temperature, and a timer through a simple touchscreen interface.

X-CLARITY™ Tissue Clearing System

The X-CLARITY™ Tissue Clearing System II is an all-in-one, easy-to-use solution for electrophoretic tissue clearing. Its unique design accelerates the removal of lipids from tissues while preserving the structural integrity of the sample. Clear a whole mouse brain in just 6 hours, an astounding 8 times faster than the original technology. Users can set tissue clearing conditions through a simple and intuitive touchscreen interface. In ETC (electrophoretic tissue clearing) mode, platinum-plated electrodes generate an electric field to accelerate the removal of lipids from tissues in a highly efficient manner. A built-in temperature control system actively cools and heats buffer to maintain consistent buffer temperatures during clearing. Buffer is constantly circulated to ensure consistent buffering capacity, temperature control, and elimination of tissue clearing byproducts. This advanced system ensures efficient, rapid, and consistent tissue clearing.

Features Easy installation, Ultrafast clearing, Reliable results The new X-CLARITY II Tissue Clearing System from the imaging specialists at Logos Biosystems provides an all-in-one solution for electrophoretic tissue clearing. The groundbreaking CLARITY™ method developed by the Deisseroth Lab at Stanford University (Chung et al, Nature 497:332-337) provides unparalleled tissue samples for imaging. Combining an innovative electrode design and an integrated cooling system, the X-CLARITY™ Tissue Clearing System allows for faster and more consistent tissue clearing. Many researchers currently attempting the CLARITY™ method often encounter difficulties that range from instrument construction to poor clarifying, resulting in the loss of valuable samples. Days or even weeks spent processing a precious sample can end in frustration and disappointment. The X-CLARITY™ Tissue Clearing System greatly simplifies the CLARITY™ process, can clarify a whole adult mouse brain in under 48 hours, and dramatically increases your chances of obtaining transparent tissue samples for use in downstream labeling.   HOW IT WORKS ? Typical negative effects related with conventional electrophoretic tissue clearing methods include burning, melting, the formation of black precipitates, and bubble trapping. The main causes of these artifacts are due to irregular electric currents and poor temperature control during electrophoresis. The X-CLARITY™ dramatically reduces the negative effects associated with standard electrophoretic tissue clearing with a unique electrode design and an active cooling system. The X-CLARITY™ Tissue Clearing System is composed of four components: 1) the X-CLARITY™ ETC Controller, 2) X-CLARITY™ ETC Chamber 3) X-CLARITY™ pump and 4) X-CLARITY™ buffer reservoir. The X-CLARITY™ ETC Controller works as both a power supply and temperature controller. At the heart of the X-CLARITY™ Tissue Clearing System is the X-CLARITY™ ETC Chamber. A tissue sample is placed inside the X-CLARITY™ ETC Chamber where it undergoes electrophoresis to remove lipids. Clearing buffer is continually circulated through the chamber under temperature controlled conditions, minimizing the probability of artifacts arising due to poor temperature control. Highlights Exceptional transparency Fastest processing time Easy installation and operation Minimized artifacts No leaking, no boiling Pre-optimized protocol ACCELERATE YOUR RESEARCH WITH THE X-CLARITY™ TISSUE CLEARING SYSTEM Compared to other methods, the X-CLARITY™ Tissue Clearing System dramatically reduces tissue clearing time. A whole adult mouse brain can be cleared in under 48 hours. Depending on the thickness, mouse brain slices may be cleared much faster. Additionally, the speed of the X-CLARITY™ Tissue Clearing System makes optimizing for other organs and tissues a much easier endeavor. One of the most exciting features of the CLARITY™ technology is that, it allows for the penetration of and labeling with macromolecules such as antibodies or oligonucleotides, generating unprecedented 3D images that show the relationship between structure and function. Chemical tissue clearing technologies introduced in recent years generally do not accommodate labeling with macromolecules. CLARITY™ overcomes this limitation and even enables multiple rounds of antibody labeling. The X-CLARITY™ Tissue Clearing System also successfully produces samples that demonstrate the efficient penetration of antibodies. WHAT IS CLARITY™ ? CLARITY™ is a novel technology that produces a transparent tissues by forming a hydrogel network that supports the ultrastructure while allowing for the removal of lipids. Unlike other tissue clearing methods, the CLARITY™ uniquely enables the efficient labeling of tissues with macromolecules such as antibodies and nucleic acids, making high resolution imaging possible. CLARITY™ is expected to overcome the current limitation of light microscopy technology, which does not allow the imaging of tissues without first creating slices. CLARITY™ is the trademark of Stanford University.

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The X-CLARITY™ is a collection of systems and ready-to-use reagents developed by Logos Biosystems to make electrophoretic tissue clearing simple, rapid, and efficient.

]]> 4103 0 11 0 https://nebg.biobm.com/product/deeplabel-antibody-staining-kit-for-cleared-tissues/ Fri, 15 Mar 2024 12:56:22 +0000 https://nebg.biobm.com/?post_type=product&p=4112 DeepLabel™ Antibody Staining Kit

DeepLabel™ Antibody Staining Kit is a set of non-toxic, ready-to-use reagents optimized for use with clarified tissues for effective antibody penetration and site-specific binding. With DeepLabel™, macromolecular probes rapidly and efficiently penetrate thick, protein-dense tissues at lower antibody concentrations. DeepLabel™ facilitates homogenous antibody staining with 2.6 X greater signal-to-background than conventional staining methods. DeepLabel™ is compatible with virtually all antibodies and all cleared tissues (compatible with CLARITY, PACT, iDISCO, CUBIC, etc).

X-CLARITY™ Mounting Solution

X-CLARITY™ Mounting Solution is a high quality, cost-efficient refractive index matching solution (RIMS) modified from the recipe developed by Yang et al. The refractive index (RI) of the solution is 1.460 at 25°C and is stable over a wide temperature range. The X-CLARITY™ Mounting Solution minimizes photobleaching and preserves fluorescence signals, making it an ideal solution for clarified and labeled tissue samples.

]]>

DeepLabel™ Antibody Staining Kit enhances antibody penetration into large clarified tissues for vibrant fluorescence 3D imaging.

]]> 4112 0 12 0 https://nebg.biobm.com/product/quantom-tx-microbial-cell-counter/ Fri, 15 Mar 2024 13:02:11 +0000 https://nebg.biobm.com/?post_type=product&p=4115 Although smaller and simpler than their eukaryotic counterparts, bacteria are an incredibly diverse group of organisms that come in multiple shapes, sizes, and arrangements. Colony counting is a highly variable and unreliable, as it is only an estimate of the viable cells present. Even expensive flow cytometers and laser scanning cytometers register each particle, single or clustered, as a single event. The QUANTOM Tx™ Microbial Cell Counter is an image-based, automated cell counter that can detect individual bacterial cells in mere minutes. Its novel bacteria-optimized cell detection software can count individual bacterial cells in even the tightest clusters.

]]>

No more waiting days to count CFUs. In less than 30 seconds, the QUANTOM Tx™ scans up to 10 fields of view to produce accurate single bacterial cell counts. The advanced software can account for the varying shapes, sizes, and arrangements of different bacteria.

]]> 4115 0 33 0 https://nebg.biobm.com/product/nightshade-evo-in-vivo-plant-imaging-system/ Fri, 15 Mar 2024 15:20:16 +0000 https://nebg.biobm.com/?post_type=product&p=4119

Flexible optical setup and exquisite sensitivity

The NightSHADE evo uses an ultrasensitive back-illuminated (backlit) CCD camera with high quantum efficiency over theentire visible to near-infrared spectrum. Cooling to a delta of -100°C ensures the lowest background and highest sensitivity, even at long exposure times. In addition, the camera can be mounted either on top of the instrument to take images from above or laterally for side view images, enabling you to maintain seedlings upright in their natural vertical orientation.  

Control key environmental conditions

The NightSHADE evo helps creating a standardized environment for your experiment.

• Temperature control – temperature-controlled baseplate to keep the temperature stable at userdefined settings between 15 and 30 °C.
• Daylight simulation – 2 LED panels with 4 different colours each. LEDs are individually tunable in intensity and duration to simulate daylight with both, spectral and intensity gradients.
• Humidity control – the system can be placed into any appropriate environmental chamber.

A modular system tailored to your workflow

The NightSHADE evo provides a light-tight imaging chamber that you can set up according to your experimental needs:

• Light-tight ports are available for the introduction of lightguides, cables or tubing, e.g. to water plants inside the chamber.
• You are able to choose between different excitation devices: ringlight for single microplates or dishes, dual gooseneck for spot illumination of dedicated areas, or 4-fold floodlight for uniform illumination of large areas.
• Several sample tables are available: the turntable can be equipped with different sample holders, e.g. to analyse square Petri dishes in upright position for unattended processing of multiple samples. The anti-condensation table for up to 9 Petri dishes features temperature control in combination with a constant, circular air flow above the Petri dishes, preventing condensation in the lids.

Powerful and intuitive indiGO™ software

The user-friendly IndiGO™ software controls the instrument and makes image processing easy. It provides the following innovative features:

• Easy wavelength management: dedicated filters for both, excitation and emission are selected via a filter inventory that provides you with information about the filter properties. The software provides the option to display up to 10 different wavelength (colours) in a single experiment. This way, multiple events can be located simultaneously.
• Daylight simulation: a set of 2 LED panels, 4 colours each can be mounted at different heights inside the imaging chamber for daylight simulation. With IndiGO™ software you can control intensity and duration of each colour individually to provide homogeneous illumination up to 1,800 μE or 15,000 Lux.
• Other features: optional 21 CRF part 11 compliance, animal alignment and digital mouse atlas alignment option.

]]> 4119 0 32 0 https://nebg.biobm.com/product/nightowl-ii-lb-983-in-vivo-imaging-system/ Fri, 15 Mar 2024 15:28:17 +0000 https://nebg.biobm.com/?post_type=product&p=4128

Low background, high resolution

The NightOWL II uses an ultrasensitive back-illuminated (backlit) CCD camera with high quantum efficiency over the entire visible to near-infrared spectrum. Efficient cooling of the array (absolute -80 to -90°C depending on the room temperature) ensures very low noise, so that long exposure times can be used to integrate the signal over time and detect even weak light signals. The sensor dimensions are 13.3 x 13.3 mm, and captured images have 1024 x 1024 pixels, 13 µm width, providing high imaging resolution.

Light-tight cabinet with motor-driven camera

The NightOWL cabinet is extremely light-tight, preventing any interferences from ambient light. NightOWL is the first imager with a motor-driven camera inside the cabinet. Optimum resolution and focus of the sample are achieved by automatic positioning of the camera according to the actual sample size. The camera can be moved from a height of 50 mm to 725 mm allowing focussing on every sample size up to 250 mm. For close-ups a macro table can be used. The camera is set up with flat field and height correction. This calibration eliminates non-uniformities caused by variations in the optical path due to height, illumination or lens effects.

A modular system tailored to your workflow

The conditions required to image living organisms can be very different depending on the application. To cover a broad range of applications, Berthold Technologies provides a very flexible system and a wide variety of accessories, including:

• Various light-sources for different applications
• Light-tight ports to insert cables, fibre optics or manipulators
• Heating tables to maintain optimum body temperature
• Transilluminators
• Gas anesthesia unit
• Animal isolation chambers for 1 or 5 rodents

These devices may even be switched on and off through the software and the built-in sockets. This option enables the researcher to add more features into the cabinet. The flange option provides light-tight access to the inner part for tubing, cables or even fibre optics, e. g. for special illumination of plants.  The flange can be customized for special purposes.

Powerful and intuitive indiGO™ software

The user-friendly IndiGO™ software controls the instrument and makes image processing easy. It provides the following innovative features:

• Easy wavelength management: dedicated filters for both, excitation and emission are selected via a filter inventory that provides you with information about the filter properties. The software provides the option to display up to 10 different wavelength (colours) in a single experiment. This way, multiple events can be located simultaneously.
• Other features: optional 21 CRF part 11 compliance, animal alignment and digital mouse atlas alignment option.

]]>

The NightOWL II uses an ultrasensitive back-illuminated (backlit) CCD camera with high quantum efficiency over the entire visible to near-infrared spectrum. Efficient cooling of the array (absolute -80 to -90°C depending on the room temperature) ensures very low noise, so that long exposure times can be used to integrate the signal over time and detect even weak light signals. The sensor dimensions are 13.3 x 13.3 mm, and captured images have 1024 x 1024 pixels, 13 µm width, providing high imaging resolution.

]]> 4128 0 31 0 https://nebg.biobm.com/product/lumat-luminometer-for-tubes/ Fri, 15 Mar 2024 16:30:39 +0000 https://nebg.biobm.com/?post_type=product&p=4133

Luminescence detection at its best

Thanks to a stringent selection of photomultipliers working in photon counting mode and more than 40 years of experience building luminometers, the new Lumat offers excellent luminescence sensitivity: <1 zmol firefly luciferase and <1 amol ATP/tube (high sensitivity model) can be reliably detected.

JET injectors - highest accuracy, speed and cell-friendliness

The Lumat can be equipped with up to 2 JET injectors with variable volumes. The ultra-fast injection speed of JET-injectors eliminates any delay between injection and measurement and is critical to measure fast reactions, e.g. for flash luminescence measurements. Berthold Technologies' JET injectors use Teflon bellows for accurate and fast injections and guarantee most efficient mixing as well as extreme longevity.

• Accurate pipetting of small volumina: regardless of whether you want to inject large or small volumes, JET injectors ensure high accuracy and more than 98% accuracy over the entire volume range
• Low maintenance costs: frictionless operation for extended lifetime
• Cell-friendly performance: cell-friendly materials and negligible shear forces enable worry-free injection of cell suspensions, e.g. in Aequorin-based calcium assays

Convenient sample switching mechanism

The measurement of individual samples one after the other can be time-consuming. The Lumat has a turret mechanism that makes this job easier and increases your throughput. Thanks to its motor-driven revolving magazine mechanism based on a rotating chamber for two tubes (one for measuring and one for loading and unloading), so you can prepare and insert the next sample while the previous one is still measuring.

Powerful software

LightCompass® is a flexible software enabling easy data acquisition, analysis and reporting using Berthold Technologies' instruments. It has been optimized for different microplate and tube formats. LightCompass® features all the functions you can expect from an advanced scientific software, such as flexible creation of measurement protocols, curve fitting, qualitative analysis and compliance with FDA 21 CFR Part 11.

]]> tube luminometer that uses state-of-the-art technology based on more than 40 years of experience engineering and designing luminometers. The luminometer supports all basic luminescence technologies including:

• Flash-type Luminescence
• Glow-type Luminescence
• Bio-Luminescence
• Chemi-Luminescence

]]> 4133 0 27 0 https://nebg.biobm.com/product/junior-lb-9509-portable-luminometer/ Mon, 18 Mar 2024 03:56:55 +0000 https://nebg.biobm.com/?post_type=product&p=4137

Research-level performance in portable format

Most portable luminometers are designed only to discriminate if an area is contaminated or not, and hence offer low sensitivity and reduced dynamic range. In contrast, the Junior offers a performance similar to those of laboratory tube luminometers: it can detect less than 50 amol ATP/tube and has a dynamic range larger than 6 orders of magnitude.

Always up and running

The Junior can be operated independently from a mains supply for many hours, as it is equipped with rechargeable batteries; spare battery sets are available, so you can continue working for as long as you need. In the lab, the instrument can be connected to a mains supply thereby automatically recharging the batteries.

Easy to carry

With a weight of only 2 Kg and its small size the Junior can be carried and taken to the sampling location for measurements on site. A metal transport case providing space for the Junior and accessories is an ideal solution for outdoor use.

]]> 4137 0 25 0 https://nebg.biobm.com/2024/02/19/navigation/ Mon, 19 Feb 2024 10:40:45 +0000 https://nebg.biobm.com/2024/02/19/navigation/ ]]> 4 0 0 0 https://nebg.biobm.com/product/crocodile-4-in-one-assay-miniworkstation/ Mon, 18 Mar 2024 04:11:37 +0000 https://nebg.biobm.com/?post_type=product&p=4145 MICROPLATE ASSAY AUTOMATION MADE EASY

Many assays performed in microplates involve several washing, dispensing, incubation and shaking steps. Processing these assays manually may require an hour or more of your time, making it hard to plan other important activities while your assay is in progress. The Crocodile 4-in-one is the ideal solution to help you free up time so that you can focus on more important tasks: it is easy to install and simple to use. Furthermore, the system avoids potential complications typical when using higher throughput automation. Using the Crocodile frees you from the manual steps and eliminates the need to transfer plates between dispenser, washer, incubator and shaker. This way you can accomplish more, faster. Forget about the huge workstations you have seen in large hospitals. Crocodile is only 26.5 cm wide. With all reagent bottles placed to the left of the instrument, it requires no more than 40 cm of space in width. Crocodile 4-in-one provides the same functionality as four individual instruments but requires the bench space only of a typical ELISA reader:

1. Dispenser
2. Shaker
3. Incubator
4. Washer

Total flexibility

Crocodile will adapt to any 96 well assay. Crocodile's strength is its versatility. Not only can it be used as a powerful and flexible assay processor, offering unattended performance, its functions also can be used to replace individual devices such as plate washers and plate readers. Unlike other ELISA workstations in the market, the Crocodile can perform assays that require to seal the plate during some incubations: just insert a Manual step in the protocol and the instrument will play a sound to warn the operator, and instruct him/her to put or remove the film. And, if you need absorbance measurement, you can choose the Crocodile 5-in-one ELISA miniWorkstation instead, which offers total flexibility in wavelength choice: in addition to the preinstalled filters for ELISA (405, 450, 492 and 620 nm), 4 positions are available for custom filters.

Walkaway automation

The workstation is designed for truly unattended operation. Simply insert the sample microplate which includes standards and samples, choose the assay protocol and leave the work to Crocodile.

Convenient assay report generation

The software creates a complete report after performing the assay. It contains not only the results, but lists also all steps performed, including start and end times, temperatures, and all sorts of helpful information.

Plug and play

All functional modules are housed in one compact box. Simply connect the unit with the power outlet and a notebook or desktop computer and you are all set for analysis. The tubing for wash and waste liquids is preinstalled, ready to be introduced into the wash and waste bottles. In everyday use, preparing to use Crocodile won´t give you any headaches. Wash, waste, and reagent bottles are readily accessible, right next to the instrument. The instrument is ready for start-up within minutes.

Validation made easy

The performance validation of the Crocodile is supported by our 3Q Package: Installation Qualification, Operation Qualification and Performance Qualification steps are performed according to detailed instructions provided. The results are documented in checklists according to the specific features of the instrument.

Easy maintenance and reliable service

Maintenance is also really simple: flushing of the system and routine replacement of the tubing is made easy by the intuitive design of Crocodile. The instrument is very robust due to the small number of moving parts. In addition, our technical experts are available to help if needed, and our service is available to ensure trouble-free operation over the lifetime of the instrument.

Convenient PC software

The user-friendly software easily adapts to any 96 well assay protocol. The five different functions can be in any sequence, one or several times each (for example one to four dispensing steps or multiple washing steps). Setting of each step is flexible and intuitive. Once you have chosen an assay protocol, the screen will show a graphic of the entire assay schedule, as it will be performed by Crocodile. The optional LightCompass software is available when quantitative and qualitative data analysis is needed.

]]> 4145 0 9 0 https://nebg.biobm.com/product/crocodile-5-in-one-elisa-miniworkstation/ Mon, 18 Mar 2024 04:17:22 +0000 https://nebg.biobm.com/?post_type=product&p=4149 SMALL FOOTPRINT. RELIABLE RESULTS. COMPLETE ELISA AUTOMATION.

Running your ELISA assays without automation requires an hour or more of your attention, making it difficult to plan other important activities while your assay is being processed. Crocodile 5-in-one provides the same functionality as five individual instruments in a footprint similar to a stand-alone-reader. Using Crocodile will reduce assay times by eliminating the need to move plates between dispenser, washer, incubator, shaker and reader. This allows you to perform other important activities. Forget about the huge workstations you have seen in large hospitals. Crocodile is only 26.5 cm wide. With all reagent bottles placed to the left of the instrument, it requires no more than 40 cm of space in width. Crocodile 5-in-one provides the same functionality as five individual instruments but requires the bench space only of a typical ELISA reader:

1. Dispenser
2. Shaker
3. Incubator
4. Washer
5. Reader

Total flexibility

Crocodile will adapt to any 96 well assay. Crocodile's strength is its versatility. Not only can it be used as a powerful and flexible assay processor, offering unattended performance, its functions also can be used to replace individual devices such as plate washers and plate readers. Unlike other ELISA workstations in the market, the Crocodile can perform assays that require to seal the plate during some incubations: just insert a manual step in the protocol and the instrument will play a sound to warn the operator, and instruct him/her to put or remove the film. And, of course, you also have total flexibility in wavelength choice: in addition to the preinstalled filters for ELISA (405, 450, 492 and 620 nm), 4 positions are available for custom filters.

Walkaway automation

The workstation is designed for truly unattended operation. Simply insert the sample microplate which includes standards and samples, choose the assay protocol and leave the work to Crocodile.

Convenient assay report generation

The software creates a complete report after performing the assay. It contains not only the results, but lists also all steps performed, including start and end times, temperatures, and all sorts of helpful information.

Plug and play

All functional modules are housed in one compact box. Simply connect the unit with the power outlet and a notebook or desktop computer and you are all set for analysis. The tubing for wash and waste liquids is preinstalled, ready to be introduced into the wash and waste bottles. In everyday use, preparing to use Crocodile won´t give you any headaches. Wash, waste, and reagent bottles are readily accessible, right next to the instrument. The instrument is ready for start-up within minutes.

Validation made easy

The performance validation of the Crocodile is supported by our IQOQ Package: Installation Qualification and Operation Qualification steps are performed according to detailed instructions provided. The results are documented in checklists according to the specific features of the instrument. In addition, we provide an optional Absorbance Test Plate to check the performance of the reader.

Easy maintenance and reliable service

Maintenance is also really simple: flushing of the system and routine replacement of the tubing is made easy by the intuitive design of Crocodile. The instrument is very robust due to the small number of moving parts. In addition, our technical experts are available to help if needed, and our service is available to ensure trouble-free operation over the lifetime of the instrument.

Convenient PC software

The user-friendly software easily adapts to any 96 well assay protocol. The five different functions can be in any sequence, one or several times each (for example one to four dispensing steps or multiple washing steps). Setting of each step is flexible and intuitive. Once you have chosen an assay protocol, the screen will show a graphic of the entire assay schedule, as it will be performed by Crocodile. The optional LightCompass software is available when quantitative and qualitative data analysis is needed.

]]> ELISA assays without automation requires an hour or more of your attention, making it difficult to plan other important activities while your assay is being processed. Crocodile 5-in-one provides the same functionality as five individual instruments in a footprint similar to a stand-alone-reader. Using Crocodile will reduce assay times by eliminating the need to move plates between dispenser, washer, incubator, shaker and reader. This allows you to perform other important activities.]]> 4149 0 10 0